CD80 antibody

Principal name

CD80 antibody

Alternative names for CD80 antibody

CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1

SwissProt ID

O55202 (Rat), P33681 (Human), P42070 (Rabit), Q00609 (Mouse)

Gene ID

941 (CD80), 12519 (Cd80)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Rt (Rat), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Mouse, Rabbit, Rat, Hamster, Goat

Available applications

Flow Cytometry (F), Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), Luminex (LMNX)

Background of CD80 antibody

The vertebrate immune system has an important self check mechanism that prevents it from destroying the host own immune system and regulatory T cells are part of this regulatory suppressive control by interacting with CD80 and CD86 (1). The cytotoxic T lymphocyte antigen 4 (CTLA4) is a protein that is expressed on regulatory T lymphocytes and is involved in restraining the immune response when needed and in tachyphylaxis and tolerance. The Fox3p-CD4+ (CD25+CD4+) regulatory T cells (Tregs) express transcription factor Foxp3 suppress aberrant immune responses as seen in autoimmune diseases and allergy (2). CD80 is a co-stimulatory ligand that interacts with CD28 and cytotoxic lymphocyte antigen 4 (CTLA4) in the regulation of T cell response. CD80 along with CD86 expression on antigen presenting cells (APCs) have the ability to augment T cell responses through interactions with CD28 (3) or inhibit T cell responses through interaction with CTLA-4 (4). The CD80/CD86 interaction with CD28/CTLA-4 occurs in the contact area between T cells and APC in parallel with T cell antigen receptor interaction with MHC-peptide complexes and LFA-1 interaction with ICAM-1 (4). The CTLA4 expressed in Fox3p positive tregs is critically required for their in vivo and in vitro suppression which is mediated at least in part by CTLA4 dependent down regulation of CD80 and CD86 in antigen presenting cells.
CD80 and CD86 are cell surface markers expressed on human dendritic cells and mature B-lymphocytes and they play a critical role in stimulating T lymphocyte response by their interaction with the ligand CTLA4 and CD28. CD80 and CD86 are up-regulated during the process of dendritic cell maturation and are over expressed in variety of lymphocytic neoplasm (6). CD80 expression is selectively increases in freshly prepared spleen B cells treated with LPS for 9-12 hours and when they are exposed to X-radiation, whereas CD86 is not affected and only slight increase in CD54, major histocompatibility complex class II, CD11a or surface immunoglobulin M was observed (5). CD80/CD86 co-stimulation is important for the immune response to vesicular stomatitis virus, herpes simplex virus and influenza virus. CD80/CD86 serve a a means of adenovirus entry on human dendritic cells and the interaction of CD80/CD86 with CTLA4 is also important in long-term control of murine gammaherpesvirus-68 virus. CD80 provides a regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. CD80 is a 321 amino acid (39kDa) protein expressed on Treg.

General readings

1. Sakahiumiuchi S. et. al., Cell 133, 775, 2008.
2. Linsley, PS; Brady, W; Grosmaire, L; Aruffo, A; Damle, NK; Ledbetter, JA. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. J Exp Med. 1991;173:721.
3. Thompson, CB; Allison, JP. The emerging role of CTLA-4 as an immune attenuator. Immunity. 1997;7:445.
4. Egen, JG; Allison, JP. Cytotoxic T lymphocyte antigen-4 accumulation in the immunological synapse is regulated by TCR signal strength. Immunity. 2002;16:23.
5. Hideko Torihata, Fumio Ishikawa, Yayoi Okada, Yuriko Tanaka, Tetsuya Uchida, Toru Suguro, and Terutaka Kakiuchi. Irradiation up-regulates CD80 expression through two different mechanisms in spleen B cells, B lymphoma cells, and dendritic cells. Immunology. 2004 June; 112(2): 219–227.
6. Maeda, T; Towatari, M; Kosugi, H; Saito, H. Up-regulation of costimulatory/adhesion molecules by histone deacetylase inhibitors in acute myeloid leukemia cells.
Blood. 2000;96:3847–3856.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI2B11 (formerly 2B11)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501575) Mouse IgG2a OTI2B11 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501576) Mouse IgG2a OTI2E5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

CD80 Hamster IgG2 16-10A1 Aff - Purified Can, Ms C, F, FN, IP
0.1 mg / €160.00
  Acris Antibodies GmbH

CD80 antibody

Surface staining of RAJI human Burkitt lymphoma cell line with anti-human CD80 (MEM-233) APC. Total viable cells were used for analysis.Total viable cells were used for analysis. Mouse IgG1 MEM-233 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD80 antibody

  CD80 mouse monoclonal antibody, clone UMAB65  
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD80 mouse monoclonal antibody. (UM500055; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB65 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD80 antibody

  CD80 mouse monoclonal antibody, clone UMAB65  
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD80 mouse monoclonal antibody. (UM500055; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB65 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9)  
Anti-CD80 mouse monoclonal antibody (TA501578) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI1E9 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501681) Mouse IgG2a OTI3F1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

AP23702PU-N CD80 antibody staining of Human Node lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD80 antibody

  Hamster Anti-Mouse CD80 (B7-1) Purified (50 ug)  
Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC .  Total viable cells were used for analysis. Hamster 16-10A1 Purified Can, Ms, Por F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

CD80 antibody

  Mouse Anti-Human CD80 (B7-1) Purified (100 ug)  
CD80 Mouse 2D10.4 Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD80 antibody

  Rabbit Polyclonal Anti-CD80 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CD80 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD80 antibody

  Hamster Monoclonal anti-CD80 Antibody  
LPS-stimulated (3 days) BALB/c splenocytes stained with purified CD80 (clone 16-10A1, filled histogram)  or Armenian hamster IgG isotype control (open histogram), followed by goat anti-Armenian hamster IgG PE. Hamster IgG 16-10A1 Purified Ms F, IP, P
0.5 mg / €325.00
  OriGene Technologies, Inc.

CD80 antibody

  Mouse Monoclonal anti-CD80 Antibody  
Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 PE Mouse 2D10 Purified Hu F, IP, P, WB
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501577) Mouse IgG2b OTI2H1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI7E4 (formerly 7E4)  
Anti-CD80 mouse monoclonal antibody (TA501667) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI7E4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

  anti-CD80 mouse monoclonal antibody, clone OTI6H7 (formerly 6H7)  
Anti-CD80 mouse monoclonal antibody (TA501668) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI6H7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

  anti-CD80 mouse monoclonal antibody, clone OTI7H3 (formerly 7H3)  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD80 monoclonal antibody. Mouse IgG1 OTI7H3 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI9H1 (formerly 9H1)  
Anti-CD80 mouse monoclonal antibody (TA501679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI9H1 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

  CD80 mouse monoclonal antibody, clone OTI7H7 (formerly 7H7)  
Anti-CD80 mouse monoclonal antibody (TA501680) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI7H7 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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