CD80 antibody

Principal name

CD80 antibody

Alternative names for CD80 antibody

CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1

SwissProt ID

O55202 (Rat), P33681 (Human), P42070 (Rabit), Q00609 (Mouse)

Gene ID

12519 (Cd80), 941 (CD80)

Available reactivities

Ms (Mouse), Rt (Rat), Can (Canine), Hu (Human), Chk (Chicken), Mky (Monkey), Por (Porcine)

Available hosts

Rat, Rabbit, Hamster, Mouse, Goat

Available applications

Flow Cytometry (F), Dot blot (Dot), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Functional assay (FN), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Neutralisation (NEUT), Luminex (LMNX)

Background of CD80 antibody

The vertebrate immune system has an important self check mechanism that prevents it from destroying the host own immune system and regulatory T cells are part of this regulatory suppressive control by interacting with CD80 and CD86 (1). The cytotoxic T lymphocyte antigen 4 (CTLA4) is a protein that is expressed on regulatory T lymphocytes and is involved in restraining the immune response when needed and in tachyphylaxis and tolerance. The Fox3p-CD4+ (CD25+CD4+) regulatory T cells (Tregs) express transcription factor Foxp3 suppress aberrant immune responses as seen in autoimmune diseases and allergy (2). CD80 is a co-stimulatory ligand that interacts with CD28 and cytotoxic lymphocyte antigen 4 (CTLA4) in the regulation of T cell response. CD80 along with CD86 expression on antigen presenting cells (APCs) have the ability to augment T cell responses through interactions with CD28 (3) or inhibit T cell responses through interaction with CTLA-4 (4). The CD80/CD86 interaction with CD28/CTLA-4 occurs in the contact area between T cells and APC in parallel with T cell antigen receptor interaction with MHC-peptide complexes and LFA-1 interaction with ICAM-1 (4). The CTLA4 expressed in Fox3p positive tregs is critically required for their in vivo and in vitro suppression which is mediated at least in part by CTLA4 dependent down regulation of CD80 and CD86 in antigen presenting cells.
CD80 and CD86 are cell surface markers expressed on human dendritic cells and mature B-lymphocytes and they play a critical role in stimulating T lymphocyte response by their interaction with the ligand CTLA4 and CD28. CD80 and CD86 are up-regulated during the process of dendritic cell maturation and are over expressed in variety of lymphocytic neoplasm (6). CD80 expression is selectively increases in freshly prepared spleen B cells treated with LPS for 9-12 hours and when they are exposed to X-radiation, whereas CD86 is not affected and only slight increase in CD54, major histocompatibility complex class II, CD11a or surface immunoglobulin M was observed (5). CD80/CD86 co-stimulation is important for the immune response to vesicular stomatitis virus, herpes simplex virus and influenza virus. CD80/CD86 serve a a means of adenovirus entry on human dendritic cells and the interaction of CD80/CD86 with CTLA4 is also important in long-term control of murine gammaherpesvirus-68 virus. CD80 provides a regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. CD80 is a 321 amino acid (39kDa) protein expressed on Treg.

General readings

1. Vasilevko V, Ghochikyan A, Holterman MJ, Agadjanyan MG: CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA. DNA Cell Biol. 2002 Mar;21(3):137-49.
2. Yadav D, Judkowski V, Flodstrom-Tullberg M, Sterling L, Redmond WL, Sherman L, Sarvetnick N. B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against
pancreatic islets. J Immunol. 2004 Sep 15;173(6):3631-9.
3. Thomas IJ, Petrich de Marquesini LG, Ravanan R, Smith RM, Guerder S, Flavell RA, Wraith DC, Wen L, Wong FS. CD86 has sustained costimulatory effects on CD8 T cells. J Immunol. 2007 Nov 1;179(9):5936-46.
4. Eri R, Kodumudi KN, Summerlin DJ, Srinivasan M. Suppression of colon inflammation by CD80 blockade: Evaluation in two murine models of inflammatory bowel disease. Inflamm Bowel Dis. 2008 Jan 9
5. Campioni D, Moretti S, Ferrari L, Punturieri M, Castoldi GL, Lanza F.: Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with
hematologic disorders: correlation with bone marrow microenvironment. Haematologica. 2006 Mar;91(3):364-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD80 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501575) Mouse IgG2a OTI2B11 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501576) Mouse IgG2a OTI2E5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

CD80 Hamster IgG2 16-10A1 Aff - Purified Can, Ms C, F, FN, IP
0.1 mg / €160.00
  Acris Antibodies GmbH

CD80 antibody

Surface staining of RAJI human Burkitt lymphoma cell line with anti-human CD80 (MEM-233) APC. Total viable cells were used for analysis.Total viable cells were used for analysis. Mouse IgG1 MEM-233 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD80 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD80 mouse monoclonal antibody. (UM500055; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB65 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD80 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD80 mouse monoclonal antibody. (UM500055; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB65 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD80 antibody

Anti-CD80 mouse monoclonal antibody (TA501578) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI1E9 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501681) Mouse IgG2a OTI3F1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

AP23702PU-N CD80 antibody staining of Human Node lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD80 antibody

Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC .  Total viable cells were used for analysis. Hamster 16-10A1 Purified Can, Ms, Por F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

CD80 antibody

CD80 Mouse 2D10.4 Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD80 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501577) Mouse IgG2b OTI2H1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Anti-CD80 mouse monoclonal antibody (TA501667) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI7E4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Anti-CD80 mouse monoclonal antibody (TA501668) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI6H7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD80 monoclonal antibody. Mouse IgG1 OTI7H3 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Anti-CD80 mouse monoclonal antibody (TA501679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI9H1 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Anti-CD80 mouse monoclonal antibody (TA501680) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI7H7 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

Anti-CD80 mouse monoclonal antibody (TA501682) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80(RC206540). Mouse IgG1 OTI10D8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD80 antibody

CD80 Luminex ELISA with 2B11 Capture (TA600489) and 2E5 Detection (TA700490) Antibodies. Substrate used: Recombinant Human CD80 (TP306540) Mouse IgG1 OTI2B11 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD80 antibody

CD80 Luminex ELISA with 1E9 Capture (TA600490) and 2B11 Detection (TA700489) Antibodies. Substrate used: Recombinant Human CD80 (TP306540) Mouse IgG1 OTI1E9 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

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