CD86 antibody

Principal name

CD86 antibody

Alternative names for CD86 antibody

CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1, T-lymphocyte activation antigen CD86, CTLA-4 counter-receptor B7.2

SwissProt ID

O35531 (Rat), P42071 (Rabit), P42081 (Human), P42082 (Mouse)

Gene ID

12524 (Cd86), 942 (CD86), 56822

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Mky (Monkey), Bab (Baboon), Chk (Chicken)

Available hosts

Rat, Mouse, Rabbit

Available applications

Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Functional assay (FN), Neutralisation (NEUT)

Background of CD86 antibody

B7-1 and B7-2 are transmembrane glycoproteins of the immunoglobulin superfamily that are expressed, along with the receptors CD28 and CTLA4, by antigen-presenting cells, and along with these receptors, constitute crucial co-stimulatory pathways for T and B cell regulatory responses. As members of the B7 family, B7-1 and B7-2 play principal roles in immunity, activating immune response and maintaining immune tolerance through engagement with CD28 and CTLA4. Co-stimulatory signals generated by B7-1 and B7-2 interactions with CD28 serve to stimulate T cell activation and prevent anergy through the amplification of T cell receptor (TCR) signaling. In contrast, interactions of the ligands with CTLA-4 serves to maintain T cell homeostasis and self-tolerance through the disruption of stimulatory signaling from B7 isoform bound CD28 complexes, and by inducing powerful inhibitory signals in T cells. B7-1 plays an important role in immune response through its amplification and regulation of T cell activity at peripheral inflammation sites. B7-1, like CTLA-4, is, however, only poorly expressed on resting dendritic cells, and its up-regulation is, therefore, considerably delayed upon immune activation. Conversely, B7-2 and CD28 are constitutively expressed by resting hematopoietic and T cells, respectively, and as a result are able to rapidly induce up-regulation upon immune activation, making them critical to the early co-stimulatory signaling of immune response. Both B7-1 and B7-2 have been shown to demonstrate co-stimulatory activity in T-cell proliferation in vitro and elicit enhanced antitumor immune response in vivo.

General readings

1. Hathcock, K., G. Laszlo, C. Pucello, O. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631.
2. Freeman, G.J., F. Borriello, R.J. Hodes, H. Reiser, K.S. Hathcock, G. Laszlo, A.J. McKnight, J. Kim, L. Du, D.B. Lombard, G.S. Gray, L.M. Nadler,
and A.H. Sharpe. 1993. Science 262:907.
3. Inaba, K. M. Witmer-Pack, M. Inaba, K.S. Hathcock, H. Sakuta, et al. 1994. J. Exp. Med. 180:1849.
4. Larsen, C.P., S.C. Ritchie, R. Hendrix, P.S. Linsley, K.S. Hathcock, R.J. Hodes, R.P. Lowry, and T.C. Pearson. 1994. J. Immunol. 152:5208.
5. Hathcock, K.S., G. Laszlo, C. Pucillo, P. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631.
6. Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. J. Immunol. 150:5252.
7. Hathcock, K., G. Laszlo, H. Dickler, J. Bradshaw, P. Linsley, and R Hodes. 1993. Science 262:905.
8. Thompson, C.B. 1995. Cell 978:982.
9. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1994. Immuol. Today 15:321.
10. Han, S. et. al. 1995. J. Immunol. 155:556.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD86 antibody

Surface staining of LPS-stimulated rat splenocytes with Anti-Rat CD86 (B7-2) FITC (left) and PE (right).  Autofluorescence is shown via open histogram.  Total viable cells were used for analysis. Mouse 24F Purified Rt F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

CD86 antibody

Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Purified Anti-Mouse CD86 (B7-2) (filled histogram) followed by Anti-Rat IgG FITC .  Total viable cells were used for analysis. Rat GL1 Purified Ms F, IP, P
0.1 mg / €130.00
  OriGene Technologies, Inc.

CD86 antibody

Staining of 2-day unstimulated (left) or LPS-stimulated (right) C57BL/6 splenocytes with 0.25 ug of Rat IgG2b K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD86 (B7-2) Purified (filled histogram) followed by Anti-Rat IgG FITC .  Total viable cells were used for analysis. Rat PO3.1 Purified Ms F
0.1 mg / €130.00
  OriGene Technologies, Inc.

CD86 antibody

Western blot analysis of extracts of various cell lines,using CD86 antibody. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD86 antibody

CD86 Mouse IgG2a B72-H2 Purified Hu C, F
0.1 mg / €410.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2b PO3 FITC Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2b PO3 Purified Ms F
25 µg / €170.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2b PO3 PE Ms F
100 Tests / €300.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2b PO3 PE Ms F
25 Tests / €210.00
  Acris Antibodies GmbH

CD86 antibody

Western blot analysis using CD86 antibody Cat.-No AM06519SU-N against L1210 (1) and MOLT-4 (2) cell lysate. Mouse IgG1 1B3 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2a GL1 APC Ms F
0.1 mg / €340.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2a GL1 Biotin Ms F
0.5 mg / €320.00
  Acris Antibodies GmbH

CD86 antibody

Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Rat anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 µg/10e6 cells. Rat IgG2a GL1 FITC Ms F
0.5 mg / €330.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2a GL1 Low Endotoxin Ms F
0.5 mg / €350.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2a GL1 Purified Ms C, F, FN, IP
0.5 mg / €290.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2a GL1 PE-Cy7 Ms F
0.1 mg / €340.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2a GL1 PE Ms F
0.2 mg / €350.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2a GL1 PE Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2b 2D10 APC Ms F
0.1 mg / €340.00
  Acris Antibodies GmbH

CD86 antibody

CD86 Rat IgG2b 2D10 Biotin Ms C, F, IP
0.5 mg / €320.00
  Acris Antibodies GmbH

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