CD9 antibody

Principal name

CD9 antibody

Alternative names for CD9 antibody

MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen, Leukocyte antigen MIC3, Motility-related protein, Cell growth-inhibiting gene 2 protein

SwissProt ID

P21926 (Human), P30409 (Chlae), P30932 (Bovin), P40239 (Felca), P40240 (Mouse), P40241 (Rat), Q8WMQ3 (Pig)

Gene ID

928 (CD9), 280746, 493874, 12527 (Cd9)

Available reactivities

Hu (Human), Can (Canine), Fe (Feline), Mky (Monkey), Rb (Rabbit), Bov (Bovine), Eq (Equine), Por (Porcine), Sh (Sheep), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunohistochemistry (IHC)

Background of CD9 antibody

The SN4 monoclonal antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignant phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in alpha-granules and through association with the integrin alphaIIb/betaIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additionally, it has been demonstrated that CD9 is able to provide a co-stimulatory signal for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the SN4 monoclonal antibody partially cross-blocks binding of another anti-human CD9 monoclonal antibody, MM2/57.

General readings

1. Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65.
2. Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7.
3. Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers
Arch. 2007 Nov;455(2):283-96.
4. Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9.
5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD9 antibody

Formalin-Fixed, Paraffin-Embedded Human Prostate tissue stained with CD9 Antibody Cat.-No AM31711PU-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG2b n.a Purified Bov, Can, Eq, Fe, Hu, Mky, Por, Rb C, F, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD9 (Center) antibody

Western blot analysis of CD9 Antibody (Center) in HepG2 cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CD9 mouse mAb with DAB staining. Mouse IgG1 5G6 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 APC Ms F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 Biotin Ms F
0.1 mg / €180.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 PE Ms F
0.1 mg / €220.00
  Acris Antibodies GmbH

CD9 antibody

Immunohistochemistry: CD9 Antibody (EPR2949) [NBP1-40752] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD9 Rabbit IgG EPR2949 Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

CD9 (N-term) antibody

Western blot analysis of CD9 Antibody (N-term) (Cat.#TA302057) in Jurkat cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

Anti-CD9 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. Mouse IgG1 SN4 C3-3A2 Aff - Purified Hu C, F, P
50 µg / €345.00
  OriGene Technologies, Inc.

CD9 antibody

Staining of C57Bl/6 bone marrow cells with 0.5 ug of Rat IgG2a Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD9 Purified (filled histogram) followed by Anti-Rat IgG PE .  Cells in the large scatter population were used for analysis. Rat eBioKMC8 (KMC8) Purified Ms F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD9 antibody

Staining of normal human peripheral blood cells with 0.25 ug of  Mouse IgG11 K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD9 Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Total viable cells were used for analysis. Mouse eBioSN4 (SN4 C3-3A2) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD9 antibody

Predicted band size: 25 kDa . Positive control: Jurkat, L1210 and TPH-1 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: L1210 cell lysate Lane3: TPH-1 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD9 antibody

Western blot analysis of extracts of various cell lines, using CD9 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

Western blot analysis of CD9 expression in COLO cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

ELISA: CD9 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

AM08351PU-N at 10 µg/ml staining CD19 in Human Skin by Immunohistochemistry, Formalin-Fixed Paraffin-Embedded tissue. Mouse IgG1 HI9a Aff - Purified Bov, Can, Eq, Hu, Rb, Sh F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 Purified Ms F, ICC/IF, IP, WB
0.1 mg / €170.00
  Acris Antibodies GmbH

CD9 antibody

Flow cytometric analysis of CD9 expression on mouse born marrow cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26477AF-N to the cells. Rat IgG2b JF9 Azide Free Ms F
0.1 mg / €320.00
  Acris Antibodies GmbH

CD9 antibody

Flow cytometric analysis of mouse CD9 expression on mouse bone marrow cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26477FC-N to the cells. Rat IgG2b JF9 FITC Ms F, IP
0.1 ml / €340.00
  Acris Antibodies GmbH

CD9 antibody

Flow cytometric analysis of CD9 expression on human lymphocyte (R1), monocyte (R2) and granulocyte (R3) using AM26546AF-N. Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26546AF-N to the cells. Mouse IgG1 10H6 Azide Free Hu F
0.1 mg / €340.00
  Acris Antibodies GmbH

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