CD9 antibody

Principal name

CD9 antibody

Alternative names for CD9 antibody

MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen, Leukocyte antigen MIC3, Motility-related protein, Cell growth-inhibiting gene 2 protein

SwissProt ID

P21926 (Human), P30409 (Chlae), P30932 (Bovin), P40239 (Felca), P40240 (Mouse), P40241 (Rat), Q8WMQ3 (Pig)

Gene ID

928 (CD9), 280746, 12527 (Cd9), 24936

Available reactivities

Hu (Human), Can (Canine), Fe (Feline), Mky (Monkey), Rb (Rabbit), Bov (Bovine), Eq (Equine), Por (Porcine), Ms (Mouse), Rt (Rat), Sh (Sheep)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunohistochemistry (IHC)

Background of CD9 antibody

The eBioSN4 monoclol antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignt phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in α-granules and through association with the integrin αIIb/βIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additiolly, it has been demonstrated that CD9 is able to provide a co-stimulatory sigl for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the eBioSN4 monoclol antibody partially cross-blocks binding of another anti-human CD9 monoclol antibody, MM2/57.

General readings

III.Workshop on Ruminant Leukocyte Antigens: Sopp P, Howard CJ.: Investigation of PC36 (BoCD45R). Vet Immunol Immunopathol. 1996 Aug;52(4):271-4.
Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens.
Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L,
Llanes D.: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies.
Vet Immunol Immunopathol. 1996 Aug;52(4):363-70.
Naessens J, Howard CJ, Hopkins J.: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
Tomaskova J, Dusinsky R, Horovska L, Simon M.: A set of monoclonal antibodies specific for bovine cell surface molecule CD9. Folia Biol (Praha). 1999;45(5):225-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD9 antibody

Formalin-Fixed, Paraffin-Embedded Human Prostate tissue stained with CD9 Antibody Cat.-No AM31711PU-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG2b n.a Purified Bov, Can, Eq, Fe, Hu, Mky, Por, Rb C, F, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD9 (Center) antibody

  Rabbit Polyclonal Antibody against CD9 (Center)  
Western blot analysis of CD9 Antibody (Center) in HepG2 cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CD9 mouse mAb with DAB staining. Mouse IgG1 5G6 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 APC Ms F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 Biotin Ms F
0.1 mg / €180.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 PE Ms F
0.1 mg / €220.00
  Acris Antibodies GmbH

CD9 (N-term) antibody

  Rabbit Polyclonal Antibody against CD9 (N-term)  
Western blot analysis of CD9 Antibody (N-term) (Cat.#TA302057) in Jurkat cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

  Anti-CD9 Mouse Monoclonal Antibody  
Anti-CD9 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. Mouse IgG1 SN4 C3-3A2 Aff - Purified Hu C, F, P
50 µg / €345.00
  OriGene Technologies, Inc.

CD9 antibody

  Rat Anti-Mouse CD9 Purified (100 ug)  
Staining of C57Bl/6 bone marrow cells with 0.5 ug of Rat IgG2a Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD9 Purified (filled histogram) followed by Anti-Rat IgG PE .  Cells in the large scatter population were used for analysis. Rat eBioKMC8 (KMC8) Purified Ms F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD9 antibody

  Mouse Anti-Human CD9 Purified (100 ug)  
Staining of normal human peripheral blood cells with 0.25 ug of  Mouse IgG11 K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD9 Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Total viable cells were used for analysis. Mouse eBioSN4 (SN4 C3-3A2) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD9 antibody

  Anti-CD9 Rabbit Polyclonal Antibody  
Predicted band size: 25 kDa . Positive control: Jurkat, L1210 and TPH-1 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: L1210 cell lysate Lane3: TPH-1 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD9 antibody

  Rabbit anti-CD9 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CD9 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

  Rabbit Polyclonal Anti-CD9 Antibody  
Western blot analysis of CD9 expression in COLO cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

  Mouse Monoclonal anti-CD9 Antibody  
BT474 breast cancer cell line was stained with anti-human CD9, detected with anti-mouse DyLighttrade; 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute Mouse HI9a Purified Hu F
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD9 antibody

  Mouse monoclonal Anti-CD9 Clone P1/33/2  
CD9 Mouse IgG1 P1/33/2 Purified Hu F, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

  Rabbit Polyclonal CD9 Antibody  
ELISA: CD9 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD9 antibody

AM08351PU-N at 10 µg/ml staining CD19 in Human Skin by Immunohistochemistry, Formalin-Fixed Paraffin-Embedded tissue. Mouse IgG1 HI9a Aff - Purified Bov, Can, Eq, Hu, Rb, Sh F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD9 antibody

Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. Rat IgG1 EM-04 Purified Ms F, ICC/IF, IP, WB
0.1 mg / €170.00
  Acris Antibodies GmbH

CD9 antibody

Flow cytometric analysis of CD9 expression on mouse born marrow cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26477AF-N to the cells. Rat IgG2b JF9 Azide Free Ms F
0.1 mg / €330.00
  Acris Antibodies GmbH

CD9 antibody

Flow cytometric analysis of mouse CD9 expression on mouse bone marrow cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26477FC-N to the cells. Rat IgG2b JF9 FITC Ms F, IP
0.1 ml / €350.00
  Acris Antibodies GmbH

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