CD90 antibody

Principal name

CD90 antibody

Alternative names for CD90 antibody

Thy-1, THY1, CDw90, Thy-1 membrane glycoprotein

SwissProt ID

O62643 (Macmu), P01830 (Rat), P01831 (Mouse), P04216 (Human), P15808 (Dicdi), Q07212 (Chick)

Gene ID

7070 (THY1), 21838 (Thy1), 24832

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Can (Canine), Sh (Sheep), Eq (Equine), Gorilla

Available hosts

Rabbit, Rat, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F), Cytotoxicity Assay (CT), Frozen Sections (C), Functional assay (FN), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CD90 antibody

CD90 is also known as Thy-1 antigen and a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kD) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and <1% of CD3+CD4+ lymphocytes in peripheral circulation. It is also expressed by human lymph node HEV endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines.

General readings

1. Mason, D.W. and Williams, A.F. (1980) The Kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem.J. 187: 1-20
2. Campbell, D.G. et al. (1981) Rat brain Thy-1 glycoprotein. Biochem. J. 195: 15-30
3. Bukovsky, A. et al. (1983) The localization of Thy-1.1, MRC OX 2 and Ia antigens in the rat ovary and fallopian tube. Immunology. 48:587-595
4. Lee, W. et al. (1998) Thy-1, a novel marker for angiogenesis up regulated by inflammatory cytokines. Circ. Res. 82: 845 - 851.
5. Jeng, C .J. et al. (1998) Thy-1 is a component common to multiple populations of synaptic vesicles. J. Cell.
Biol. 140(3):685-98.
6. Banerjee, S.A. et al. (1997) An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner. J. Neurosci. 17: 2756-2765.
7. Kawachi, H. et al. (1992) Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen. Clin. Exp. Immunol. 88: 399-404.
8. Tamura, M. et al. (1996) Enhanced Glomerular Profilin Gene and Protein Expression in Experimental Mesangial Proliferative Glomerulonephritis. Biochem Biophys Res Comm.
9. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 18:1389-98.
10. Biermann, J. et al. (2011) Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells. Mol Vis. 17: 395-403.
11. Keller, R.K. et al. (2004) Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome. J Lipid Res. 45: 347-55.
12. Ohashi, N. et al. (2010) Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways. Hypertens Res. 33:1174-81.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD90.2 antibody

CD90.2 Rat IgG2b 30-H12 APC Ms F
0.1 mg / €320.00
  Acris Antibodies GmbH

CD90.2 antibody

CD90.2 Rat IgG2b 30-H12 Biotin Ms F
0.5 mg / €270.00
  Acris Antibodies GmbH

CD90.2 antibody

Immunofluorescent Staining: BALB/c splenocytes were double-stained with Rat anti-Mouse CD90.2-FITC and Rat anti-Mouse CD19-PE. Small lymphocytes were then gated and analyzed on a FACScan(TM) flowcytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells. Rat IgG2b 30-H12 FITC Ms F
0.5 mg / €320.00
  Acris Antibodies GmbH

CD90.2 antibody

Immunofluorescent Staining: BALB/c splenocytes were double-stained with Rat anti-Mouse CD90.2-FITC and Rat anti-Mouse CD19-PE. Small lymphocytes were then gated and analyzed on a FACScan(TM) flowcytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells. Rat IgG2b 30-H12 FITC Ms F
0.1 mg / €170.00
  Acris Antibodies GmbH

CD90.2 antibody

CD90.2 Rat IgG2b 30-H12 Low Endotoxin Ms F
0.5 mg / €350.00
  Acris Antibodies GmbH

CD90.2 antibody

CD90.2 Rat IgG2b 30-H12 Purified Ms C, F, FN
0.5 mg / €230.00
  Acris Antibodies GmbH

CD90.2 antibody

CD90.2 Rat IgG2b 30-H12 PE Ms F
0.2 mg / €240.00
  Acris Antibodies GmbH

CD90.2 antibody

CD90.2 Rat IgG2b 30-H12 PE Ms F
0.1 mg / €190.00
  Acris Antibodies GmbH

CD90.2 antibody

Flow cytometry / FACS analysis: CD90.2 Antibody (30-H12) [NBP1-28036] - BALB/c splenocytes were double-stained with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19- R-PE. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). Rat IgG2b 30-H12 Purified Ms C, F, P
0.5 mg / €360.00
  Novus Biologicals Inc.

CD90.2 antibody

Flow cytometry: CD90.2 Antibody (30-H12) [NBP1-28037] - 0.3 ug/10^6 BALB/c splenocytes were double-stained with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed on a flow cytometer Rat IgG2b 30-H12 FITC Ms C, F, P
0.5 mg / €320.00
  Novus Biologicals Inc.

CD90.2 antibody

Flow cytometry / FACS analysis: CD90.2 Antibody (30-H12) [R-Phycoerythrin] [NBP1-28039] - BALB/c splenocytes were double-stained with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19- R-PE. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). Rat IgG2b 30-H12 PE Ms C, F, P
0.2 mg / €250.00
  Novus Biologicals Inc.

CD90.2 antibody

Flow cytometry / FACS analysis: CD90.2 Antibody (30-H12) [Allophycocyanin] [NBP1-28040] - BALB/c splenocytes were double-stained with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19- R-PE. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). Rat IgG2b 30-H12 Phyco Ms C, F, P
0.1 mg / €320.00
  Novus Biologicals Inc.

CD90 antibody

Staining of rat thymus cells with Mouse anti Rat CD90 (THY-1.1)-FITC Mouse IgG1 OX-7 FITC GP, Ms, Rb, Rt F
0.1 mg / €420.00
  Acris Antibodies GmbH

CD90 antibody

Staining of rat thymus cells with Mouse anti Rat CD90 (THY-1.1)-FITC Mouse IgG1 OX-7 FITC GP, Ms, Rb, Rt F
50 µg / €290.00
  Acris Antibodies GmbH

CD90 antibody

Staining of rat thymus cells with Mouse anti Rat CD90 (THY-1.1)-FITC Mouse IgG1 OX-7 FITC GP, Ms, Rb, Rt F
25 µg / €180.00
  Acris Antibodies GmbH

CD90 antibody

CD90 Rat IgG2b YKIX337.217 Purified Can F, IP
0.25 mg / €550.00
  Acris Antibodies GmbH

CD90 (Thy1.2) antibody

CD90 Mouse IgG3 1aG4/C5 Azide Free Ms F, FN, ICC/IF, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD90 antibody

TA305663 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD90 antibody

Western blot analysis of extracts from HuvEc cells, using THY1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD90 antibody

Anti-THY1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG1 5E10 Aff - Purified Hu, Mky C, F, IP, P
50 µg / €345.00
  OriGene Technologies, Inc.

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