CD95 / FAS antibody

Principal name

CD95 / FAS antibody

Alternative names for CD95 / FAS antibody

FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptor superfamily member 6

SwissProt ID

O77736 (Pig), P25445 (Human), P25446 (Mouse), P51867 (Bovin), Q63199 (Rat)

Gene ID

355 (FAS), 14102 (Fas), 246097, 1090893, 8794 (TNFRSF10C)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Por (Porcine), Bov (Bovine), Can (Canine), Hst (Hamster), Mky (Monkey), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Rhesus Mky (Rhesus Monkey)

Available hosts

Mouse, Rabbit, Rat, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Functional assay (FN), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), EPair for Elisa (EPair), Neutralisation (NEUT)

Background of CD95 / FAS antibody

Homeostasis of multicellular organisms is controlled not only by the proliferation and differentiation of cells but also by cell death. The death of cells during embryogenesis, endocrine-dependent tissue atrophy, metamorphosis, a variety of pathologic conditions, and normal tissue turnover, is called programmed cell death (PCD). Most PCD proceeds by apoptosis, a process that includes condensation and segmentation of nuclei, condensation and fragmentation of the cytoplasm, and often extensive fragmentation of chromosomal DNA into nucleosome units. Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well-studied receptors are CD95/ Fas/ Apo-1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these receptors results in activation of a family of cysteine proteases known as caspases. However, Fas-mediated death occurs much more rapidly than that triggered by the TNFR1. The action of Fas is mediated via FADD (Fas-associated death domain)/ MORT1, an adapter protein that has a death domain at its C-terminus and binds to the cytoplasmic death domain of Fas. Human CD95/ Fas/ Apo-1 antigen is a single transmembrane glycoprotein receptor (281 amino acids, 33 kDa). An integral membrane protein, has been identified as the Fas ligand (Fas ligand, FasL, CD95L). FasL is a type II transmembrane glycoprotein and is a member of the TNF family, which includes TNF-alpha and the alpha and beta chains of lymphotoxin (LT), CD40 ligand and CD30 ligand. The amino acid sequences of human and murine FasL are 76.9% identical, and they are not species-specific. Membrane bound FasL (mFasL) is a 40 kDa protein, while the active soluble form of FasL (sFasL) was identified as a 26 kDa protein in the supernatant of activated peripheral T cells and cultured cells transfected with the full-length FasL DNA. FasL has four potential N-glycosylation sites which appear to be variably used. Consequently, the apparent MW of FasL may vary, per glycosylation and breakdown patterns in a certain preparation. Engagement of Fas by its ligand, results in the rapid induction of PCD in susceptible cells. This process bypasses the usual long sequence of signaling enzymes and immediately activates preexisting caspases. FasL is expressed on activated T cells, whereas Fas is expressed on various types of cells. The cellular pathways that control apoptosis are critical to the maturation, selection, and survival of lymphocytes. Apoptosis or cell suicide is the physiological mode of lymphoid cell death in circumstances like negative selection of T cells in the thymus, ligation of CD4 and CD3 in mature T cells, downregulation of the immune response, clonal deletion of B cells by antigen, death of killer cell targets, cytokine-mediated killing, and tumor regression. Fas is also expressed on a number of lymphoma cell lines, on Epstein-Barr virus-transformed B lymphoblasts, and on a proportion of activated B and T cells. The production of excess soluble Fas protein would prevent cells from undergoing Fas ligand induced apoptosis and thereby permit tumor cells to escape immunosurveillance. The activation of mature T cells with phorbol myristic acetate (PMA) and ionomycin, concanavalin A (Con A) or anti-CD3, induces FasL gene expression. Herpes Simplex virus type 2 (HSV-2) but not HSV-1, potentially inhibits FasL surface expresssion in infected cells and thereby suppresses FasL-mediated cell death. Antibodies reacting specifically with Fas ligand are useful tools in the study of the intracellular pathways leading from membrane receptor engagement to apoptotic cell death, the tissue distribution and developmental expression pattern of Fas ligand, and its essential role during mammalian development especially in immune system homeostasis.

General readings

1. Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar
16;17(6):1675-87.
2. Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170(12):6209-16.
3. Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines. Blood. 2005 Sep 15;106(6):2033-41.
4. Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63.
5. Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A.: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem. 2005 Jun 17;280(24):22856-67. Epub 2005 Mar 8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD95 / FAS antibody

Flow Cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-Human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red: Isotype Mouse IgG1 control staining, Black: standard anti-CD95 monoclonal antibody, Green: anti-CD95 (LT95). A: JURKAT Human peripheral blood T cell leukemia cell line. B: RAMOS Human Burkitt lymphoma cell line. C: CEM Human leukemia cell line. D: MOLT-4 Human acute lymphoblastic T cell leukemia cell line. Mouse IgG1 LT95 Purified Hu F, P
0.1 mg / €210.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indica tes the reaction of isotypic control to the cells . Shaded histogram indicates the reaction of AM26668FC-N to the cells. Mouse IgG1 UB2 FITC Hu F
50 Tests / €380.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26668PU-N to the cells. Mouse IgG1 UB2 Aff - Purified Hu C, ICC/IF, P
0.1 mg / €430.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26668RP-N to the cells. Mouse IgG1 UB2 PE Hu F
50 Tests / €410.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Western blot analysis of extracts of SW480 cell lines, using FAS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Immunofluorescence analysis of Hela cells using FAS antibody Cat.-No AM06730PU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 4F8H6 Purified Hu E, F, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Western blot analysis using FAS antibody Cat.-No AM06732PU-N against human FAS (AA: 87-278) recombinant protein (Expected MW is 47.2 kDa) Mouse IgG1 4F8D6 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD95 / FAS (C-term) antibody

Human colon stained with anti-CD95/Fas antibody Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD95 / FAS (C-term) antibody

Human colon stained with anti-CD95/Fas antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD95 / FAS (C-term) antibody

Human colon stained with anti-CD95/Fas antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD95 / FAS antibody

TA305714 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Western blot analysis of extracts from LOVO cells, using Fas antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Staining of BALB/c thymocytes with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified  (open histogram) or 0.5 ug of Anti-Mouse CD95 (APO-1/Fas) Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Total viable cells were used for analysis. Mouse 15A7 Purified Ms F
50 µg / €120.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

The Jurkat cell line was treated for 6 hours with medium alone (left) or 0.1 ug/mL of Anti-Human CD95 (APO-1/Fas) Functional Grade Purified (right). Induction of apoptosis in these cells was determined by staining with Propidium Iodide and Anti-BrdU-FITC using the Apo-BrdU Apoptosis Detection Kit . Mouse EOS9.1 Purified Hu F
25 µg / €120.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Western blot analysis of Hela cell using TA323753(FAS Antibody) at dilution 1/550 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Predicted band size: 38 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Western blot detection of FAS(C-terminus) in Jurkat cell lysates using FAS(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa. Mouse IgG1 4C3-G7-B7-H5 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAS (RC204520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAS. Mouse IgG1 OTI1F2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD95 / FAS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAS (RC204520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAS. Mouse IgG1 OTI2C2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD95 / FAS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAS (RC215929, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAS. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

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