CD95 / FAS antibody

Principal name

CD95 / FAS antibody

Alternative names for CD95 / FAS antibody

FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptor superfamily member 6

SwissProt ID

O77736 (Pig), P25445 (Human), P25446 (Mouse), P51867 (Bovin), Q63199 (Rat)

Gene ID

355 (FAS), 14102 (Fas), 246097

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit, Rat, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Flow Cytometry (F), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Dot blot (Dot), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Neutralisation (NEUT)

Background of CD95 / FAS antibody

Apoptosis inducing protein 1(Apo-1) also known as Fas (Fibroblast-associated), CD95, and TNFRSF6(325 amino acids, 45-48 kDa), was originally identified as a cell-surface protein that binds to monoclonal antibodies that are cytolytic for various human cell lines. Fas is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of Apo on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. Fas-mediated death occurs much more rapidly than that triggered by TNFR1(Tumor necrosis factor receptor 1).

Fas (APO-1 or CD95) is a cell-surface receptor that transduces apoptotic signals from Fasligand (FasL) . It is a glycoprotein with a mass estimated at 43 to 48 kDa . Fas is a member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF), and it shares a cytoplasmic motif with TNF RI, referred to as the Â?death domainÂ?, that binds cytoplasmic signaling molecules to trigger the cytoplasmic apoptotic signal . Fas is expressed to a large extent on activated T and B lymphocytes, and on malignant lymphoid cells. To a lesser extent, Fas is expressed on cells from liver, heart, kidney, ovaries, and on many other malignant cells.

FasL, the physiological agonist for Fas, is also a transmembrane protein with homology to the TNF family in its extracellular domain. FasL is expressed primarily by activated T lymphocytes and by cells of the small intestine and lung. Mice with mutations in either Fas or FasL exhibit accumulation of activated lymphocytes and classical autoimmune symptoms, suggesting that a major function of Fas-mediated apoptosis is the elimination of activated immune cells from the peripheral circulation . Similarly, humans with autoimmune lymphoproliferative syndrome have mutations in Fas . Fas and FasL have been observed as soluble molecules in addition to their membraneassociated forms, suggesting additional complexity to regulation of this apoptotic mechanism . Soluble Fas (sFas) arises from alternatively spliced mRNA, leading to proteins with deletion or disruption of the single membrane-spanning domain . Five alternatively spliced Fas mRNAs have been described , each protein detected in the supernate of cultures of peripheral blood mononuclear cells or certain tumor cell lines. Each sFas inhibited apoptosis induced by FasL , and tumor-cell lines resistant to anti-Fas were shown to produce alternatively spliced Fas, thereby making them less sensitive to FasL. In addition, plasma Fas can arise by exfoliation of membrane vesicles, which also inhibit FasL-induced apoptosis . Serum Fas has been reported to be elevated in cancer patients , possibly originating in the tumor cell itself , and in autoimmune diseases .

General readings

Nagata, S. (1994) Adv. Immunol. 57:129.

Nagata, S. and P. Golstein (1995) Science 267:1449.

Itoh, N. et al. (1991) Cell 66:233.

Oehm, A. et al. (1992) J. Biol. Chem. 267:10709.

Cleveland, J.L. and J.N. Ihle (1995) Cell 81:479.

Crispe, I.N. (1994) Immunity 1:347.

Rieux-Laucat, F. (1995) Science 268:1347.

Fisher, G.H. (1995) Cell 81:935.

Cheng, J. et al. (1994) Science 263:1759.

Papoff, G. et al. (1996) J. Immunol. 156:4622.

Tanaka, M. et al. (1996) Nature Medicine 2:317.

Albanese, J. et al. (1998) Blood 91:3862.

Knipping, E. et al. (1995) Blood 85:1562.

Owen-Schaub, L.B. et al. (1995) Cancer Lett. 94:1.

Midis, G. et al. (1996) Cancer Res. 56:3870.

Mizutani, Y. et al. (1998) J. Urol. 160:571.

Lee, S.H. et al. (1998) Lab. Invest. 78:453.

Jodo, S. et al. (1997) Clin. Exp. Immunol. 107:89.

Jodo, S. et al. (1998) Clin. Exp. Immunol. 112:166.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD95 / FAS antibody

Flow Cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-Human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red: Isotype Mouse IgG1 control staining, Black: standard anti-CD95 monoclonal antibody, Green: anti-CD95 (LT95). A: JURKAT Human peripheral blood T cell leukemia cell line. B: RAMOS Human Burkitt lymphoma cell line. C: CEM Human leukemia cell line. D: MOLT-4 Human acute lymphoblastic T cell leukemia cell line. Mouse IgG1 LT95 Purified Hu F, P
0.1 mg / €210.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indica tes the reaction of isotypic control to the cells . Shaded histogram indicates the reaction of AM26668FC-N to the cells. Mouse IgG1 UB2 FITC Hu F
50 Tests / €390.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26668PU-N to the cells. Mouse IgG1 UB2 Aff - Purified Hu C, ICC/IF, P
0.1 mg / €440.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Flow cytometric analysis of human Fas expression on transfectant. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26668RP-N to the cells. Mouse IgG1 UB2 PE Hu F
50 Tests / €420.00
  Acris Antibodies GmbH

CD95 / FAS antibody

  FAS Rabbit Polyclonal Antibody  
Western blot analysis of extracts of SW480 cell lines, using FAS antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Immunofluorescence analysis of Hela cells using FAS antibody Cat.-No AM06730PU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 4F8H6 Purified Hu E, F, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Western blot analysis using FAS antibody Cat.-No AM06732PU-N against human FAS (AA: 87-278) recombinant protein (Expected MW is 47.2 kDa) Mouse IgG1 4F8D6 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD95 / FAS antibody

  Goat Anti-FAS / CD95 Antibody  
TA305714 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

  Rabbit polyclonal anti-FAS antibody  
Western blot analysis of extracts from LOVO cells, using Fas antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

  Mouse Anti-Mouse CD95 (APO-1/Fas) Purified (50 ug)  
Staining of BALB/c thymocytes with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified  (open histogram) or 0.5 ug of Anti-Mouse CD95 (APO-1/Fas) Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Total viable cells were used for analysis. Mouse 15A7 Purified Ms F
50 µg / €120.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

  Mouse Anti-Human CD95 (APO-1/Fas) Purified (25 ug)  
The Jurkat cell line was treated for 6 hours with medium alone (left) or 0.1 ug/mL of Anti-Human CD95 (APO-1/Fas) Functional Grade Purified (right). Induction of apoptosis in these cells was determined by staining with Propidium Iodide and Anti-BrdU-FITC using the Apo-BrdU Apoptosis Detection Kit . Mouse EOS9.1 Purified Hu F
25 µg / €120.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

  Anti-FAS Rabbit Polyclonal Antibody  
Predicted band size: 38 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

  Mouse Monoclonal FAS (C-terminus) Antibody  
Western blot detection of FAS(C-terminus) in Jurkat cell lysates using FAS(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa. Mouse IgG1 4C3-G7-B7-H5 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

  Mouse Monoclonal anti-CD95 (FAS) Antibody  
Human peripheral blood lymphocytes stained with purified EOS9.1, followed by anti-mouse IgM PE Mouse EOS9.1 Purified Hu F
0.1 mg / €280.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

  FAS mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAS (RC204520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAS. Mouse IgG1 OTI1F2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD95 / FAS antibody

  FAS mouse monoclonal antibody, clone OTI2C2 (formerly 2C2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAS (RC204520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAS. Mouse IgG1 OTI2C2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD95 / FAS antibody

  Rabbit Polyclonal Fas Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAS (RC215929, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAS. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD95 / FAS antibody

Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red – Isotype mouse IgG1 control staining. Black – standard anti-CD95 monoclonal antibody (DX2). Green – anti-CD95 (LT95). 
A – JURKAT human peripheral blood T cell leukemia cell line. B – RAMOS human Burkitt lymphoma cell line. C – CEM human leukemia cell line. D – MOLT-4 human acute lymphoblastic T cell leukemia cell line. Mouse IgG1 LT95 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD95 / FAS antibody

CD95 / FAS Mouse IgG1 LT95 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD95 / FAS antibody

Surface staining of human peripheral blood cells with anti-CD95 (LT95) PE. Mouse IgG1 LT95 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

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