CD97 antibody

Principal name

CD97 antibody

Alternative names for CD97 antibody

Leukocyte antigen CD97

SwissProt ID

P48960 (Human), Q8SQA4 (Bovin), Q9Z0M6 (Mouse)

Gene ID

976 (CD97), 26364 (Cd97)

Available reactivities

Hu (Human), Mky (Monkey), Bt (Bat), Bov (Bovine), Can (Canine), Hst (Hamster), Ms (Mouse), Op (Opossum), Por (Porcine), Prim (Primate), Rt (Rat), Bear

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of CD97 antibody

CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including hematopoietic cells. Particularly high levels of expression are also seen in the lung and the thymus gland. In the spleen, expression is detected on most myeloid cells and variable portions of T cells, B cells and NK cells. In humans, CD97 expression is upregulated during lymphocyte activation on various epithelial cancer cells including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha 5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Antibodies against CD97 are suitable for exploring various mouse genetic models of cancer, angiogenesis, and inflammation. CD97 can be proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. Multiple isoforms have been identified for this protein.

General readings

1. Visser L, de Vos AF, Hamann J, Melief MJ, van Meurs M, van Lier RA, Laman JD, Hintzen RQ. Expression of the EGF-TM7 receptor CD97 and its ligand CD55 (DAF) in multiple sclerosis. J Neuroimmunol. 2002 Nov;132(1-2):156-63.
2. Galle J, Sittig D, Hanisch I, Wobus M, Wandel E, Loeffler M, Aust G: Individual cell-based models of tumor-environment interactions: Multiple effects of CD97 on tumor invasion. Am J Pathol. 2006 Nov;169(5):1802-11.
3. Abbott RJ, Spendlove I, Roversi P, Fitzgibbon H, Knott V, Teriete P, McDonnell JM, Handford PA, Lea SM: Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55. J Biol Chem. 2007 Jul 27;282(30):22023-32.
4. Wang T, Tian L, Haino M, Gao JL, Lake R, Ward Y, Wang H, Siebenlist U, Murphy PM, Kelly K: Improved antibacterial host defense and altered peripheral granulocyte homeostasis
in mice lacking the adhesion class G protein receptor CD97. Infect Immun. 2007 Mar;75(3):1144-53.
5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Wobus M, Vogel B, Schmücking E, Hamann J, Aust G: N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding. Int J Cancer. 2004 Dec 10;112(5):815-22.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD97 (C-term) antibody

Figure 2. Staining of Mouse Colon using AP32070PU-N CD97 antibody at 1 µg/ml (wt, left lane, knock-out right lane) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by infrared fluorescence (Odyssey). Data obtained from anonymous customer. Goat Aff - Purified Ms E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD97 antibody

  Rabbit polyclonal CD97a (Cleaved-Leu530) antibody  
Western blot analysis of extracts from Jurkat cells, using CD97a (Cleaved-Leu530) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD97 antibody

  Rabbit polyclonal CD97beta (Cleaved-Ser531) antibody  
Western blot analysis of extracts from Jurkat cells, treated with etoposide (25uM, 24hours), using CD97ß (Cleaved-Ser531) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD97 (1-512) antibody

  Rabbit polyclonal anti-CD97 antibody  
WB using anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). The formation of the CD97 complex is currently under investigation. A 1:1,000 dilution of the primary antibody was used. Rabbit IgG Purified Ms E, ICC/IF, WB
0.5 mg / €419.00
  OriGene Technologies, Inc.

CD97 antibody

  Rabbit Polyclonal Anti-CD97 Antibody (N-Terminus)  
Anti-CD97 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu, Mky
50 µg / €400.00
  OriGene Technologies, Inc.

CD97 antibody

  Rabbit Polyclonal Anti-CD97 Antibody  
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (CD97 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD97 (220-270) antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Mky P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD97 antibody

Peripheral Human Granulocytes stained with Mouse Anti Human CD97-APC (SM1601APC). Mouse IgG1 MEM-180 APC Hu F
100 Tests / €430.00
  Acris Antibodies GmbH

CD97 antibody

CD97 Mouse IgG1 MEM-180 FITC Hu F
0.1 mg / €470.00
  Acris Antibodies GmbH

CD97 antibody

SM1601P staining of peripheral blood leucocytes. Mouse IgG1 MEM-180 Purified Hu C, F
0.2 mg / €590.00
  Acris Antibodies GmbH

CD97 antibody

CD97 Mouse IgG1 MEM-180 Purified Hu F, IP
0.1 mg / €220.00
  Acris Antibodies GmbH

CD97 antibody

CD97 Mouse IgG1 MEM-180 PE Hu F
100 Tests / €480.00
  Acris Antibodies GmbH

CD97 antibody

CD97 Mouse IgG1 MEM-180 PE Hu F
25 Tests / €220.00
  Acris Antibodies GmbH

CD97 (N-term) antibody

Cells expressing vector only - immunostained with target antibody Rabbit Aff - Purified Hu, Mky ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

CD97 (3rd Cytopl. Dom.) antibody

Immunohistochemical staining of Bone Marrow using anti- CD97 antibody SP4365P Rabbit Aff - Purified Bear, Bov, Bt, Can, Elephant, Hst, Hu, Ms, Op, Platypus, Por, Prim, Rt ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

CD97 (cleaved) antibody

Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using CD97 beta (Cleaved-Ser531) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

CD97 (cleaved) antibody

Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using CD97 beta (Cleaved-Ser531) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Cd97 - mouse gene knockout kit via CRISPR

Cd97 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cd97 - mouse gene knockout kit via CRISPR

Cd97 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cd97 - mouse gene knockout kit via CRISPR

Cd97 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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