CD99 / MIC2 antibody

Principal name

CD99 / MIC2 antibody

Alternative names for CD99 / MIC2 antibody

E2 antigen, 12E7, MIC2X, MIC2Y, T-cell surface glycoprotein E2, Protein MIC2

SwissProt ID

P14209 (Human), Q8VCN6 (Mouse)

Gene ID

4267 (CD99), 673094 (Cd99)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)

Background of CD99 / MIC2 antibody

The HCD99 monoclonal antibody recognizes human CD99 also known as CD99 antigen, E2 antigen, MIC2, and T-cell surface glycoprotein E2. CD99 is a type I, single chain transmembrane protein devoid of N-linked glycosylation sites that is encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, T cell leukemias and lymphomas and is absent on fetal B cells, some B cell lines, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H. The HCD99 antibody has been reported to be useful for flow cytometric detection of human CD99 and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues).

General readings

Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO
J. 1989 Nov;8(11):3253-9.
Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes. J Immunol.
1995 Jan 1;154(1):26-32.
Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J Immunol. 1997 Mar 15;158(6):2543-50.
Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium
under flow conditions. Eur J Immunol. 2000 Oct;30(10):3061-5.
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Cermak L, Simova S, Pintzas A, Horejsi V, Andera L.: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800788) Mouse IgG2b OTI5C1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800819) Mouse IgG1 OTI1F11 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800820) Mouse IgG1 OTI1A4 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800824) Mouse IgG1 OTI1D5 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800904) Mouse IgG2b OTI5C8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800907) Mouse IgG1 OTI3E8 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800908) Mouse IgG1 OTI13F10 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800910) Mouse IgG1 OTI4C6 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800916) Mouse IgG1 OTI4E1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800957) Mouse IgG2b OTI13B3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD99 / MIC2 antibody

Human pancreas stained with anti-CD99 antibody Rabbit IgG SP119 Purified Hu P
0.5 ml / €510.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Human pancreas stained with anti-CD99 antibody Rabbit IgG SP119 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Human pancreas stained with anti-CD99 antibody Rabbit IgG SP119 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Formalin-Paraffin Ewing’s sarcoma stained with CD99 / MIC2 Antibody Cat.-No AM33250SU (Clone SPM586). Mouse IgM SPM586 Supernatant Hu, Rt C, ICC/IF, P
1.0 ml / €360.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Formalin-Paraffin Ewing’s sarcoma stained with CD99 / MIC2 Antibody Cat.-No AM33250SU (Clone SPM586). Mouse IgM SPM586 Supernatant Hu, Rt C, ICC/IF, P
0.5 ml / €290.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Formalin-Paraffin Ewing’s sarcoma stained with CD99 / MIC2 Antibody Cat.-No AM33250SU (Clone SPM586). Mouse IgM SPM586 Supernatant Hu, Rt C, ICC/IF, P
0.1 ml / €150.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Formalin-Paraffin Human tonsil stained with CD99 Antibody (Clone HO36-1.1). Mouse IgM HO36-1.1 Supernatant Hu, Rt C, ICC/IF, P
1.0 ml / €360.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Formalin-Paraffin Human tonsil stained with CD99 Antibody (Clone HO36-1.1). Mouse IgM HO36-1.1 Supernatant Hu, Rt C, ICC/IF, P
0.5 ml / €290.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

Formalin-Paraffin Human tonsil stained with CD99 Antibody (Clone HO36-1.1). Mouse IgM HO36-1.1 Supernatant Hu, Rt C, ICC/IF, P
0.1 ml / €150.00
  Acris Antibodies GmbH

CD99 / MIC2 antibody

AP15457PU CD99 antibody staining of Human Pancreas. Rabbit Purified Hu P, WB
0.5 ml / €300.00
  Acris Antibodies GmbH

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