CDC16 / APC6 antibody

Principal name

CDC16 / APC6 antibody

Alternative names for CDC16 / APC6 antibody

ANAPC6, Cell division cycle protein 16 homolog, CDC16Hs, Anaphase-promoting complex subunit 6, Cyclosome subunit 6

SwissProt ID

Q13042 (Human), Q8R349 (Mouse)

Gene ID

8881 (CDC16)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of CDC16 / APC6 antibody

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle.Cdc25A, Cdc25B and Cdc25C protein Tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory Tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

General readings

General / background references:Kallio M et al. Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. J Cell Biol 141:1393-406 (1998). PubMed PMID: 9628895
Ollendorff V & Donoghue DJ The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. J Biol Chem 272:32011-8 (1997). PubMed PMID: 9405394

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC16 / APC6 antibody

Western blot analysis of extracts from HuvEc cells, using CDC16/APC6 (Ab-560) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC16 / APC6 antibody

Western blot analysis of extracts from NIH-3T3 cells, using APC6 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC16 / APC6 antibody

Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC16 / APC6 antibody

Host: Rabbit; Target Name: CDC16; Sample Tissue: 786-0 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC16 / APC6 antibody

Western blot (WB) analysis of Cdc16 antibody (Cat.-No.: AP06782PU-N) at 1/500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC16 / APC6 pThr580 (575-588) antibody

AP07554PU-N CDC16 antibody staining of Formalin-Fixed Paraffin-Embedded Human Lung, Respiratory Epithelium at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CDC16 / APC6 pThr580 antibody

Western blot using Affinity Purified anti-APC6 pT580 antibody shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35 µg of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gta-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CDC16 / APC6 antibody

Immunohistochemical analysis of human breast carcinoma tissue (Formalin- fixed  Paraffin-embedded) using CDC16 / APC6 antibody (Cat.-No. AP20213PU-N). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC16 / APC6 (N-term) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CDC16 / APC6 Control Peptide

CDC16 / APC6
50 µg / €160.00
  Acris Antibodies GmbH

CDC16 / APC6 antibody

CDC16 / APC6 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CDC16 / APC6 antibody

Western blot using CDC16 (phospho T580) polyclonal antibody ( Cat # PAB10325 ) shows detectionof a band ~ 72 kDa corresponding to phosphorylated human CDC16 ( arrowhead lane 1 ).
Lane 1 -nocodazole treated HeLa whole cell lysate.
Lane 2 -Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures.
Each lane contains approximately 35 µg of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Rabbit Hu E, ICC/IF, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

CDC16 / APC6 pSer560 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using CDC16/APC6 (Phospho-Ser560) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

CDC16 / APC6 pSer560 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using CDC16/APC6 (Phospho-Ser560) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

CDC16 / APC6 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CDC16/APC6 (Ab-560) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

CDC16 / APC6 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CDC16/APC6 (Ab-560) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

CDC16 / APC6 antibody

Western blot analysis of extracts from NIH-3T3 cells, using APC6 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CDC16 / APC6 antibody

Western blot analysis of extracts from NIH-3T3 cells, using APC6 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CDC16 / APC6 antibody

Western Blot: CDC16 [p Thr580] Antibody [NB600-889] - Western blot using Affinity Purified anti-APC6 pT580 antibody shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35 ìg of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4 degrees C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gta- Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

CDC16 / APC6 antibody

CDC16 / APC6 Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

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