CDC20 antibody

Principal name

CDC20 antibody

Alternative names for CDC20 antibody

p55CDC, Cell division cycle protein 20 homolog

SwissProt ID

P26309 (Yeast), Q12834 (Human), Q54MZ3 (Dicdi), Q5H7C0 (Pig), Q62623 (Rat), Q9JJ66 (Mouse)

Gene ID

991 (CDC20), 107995 (Cdc20)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CDC20 antibody

The cell division cycle demands accuracy to avoid the accumulation of genetic damage. This process is controlled by molecular circuits called "checkpoints" that are common to all eukaryotic cells. Checkpoints monitor D integrity and cell growth prior to replication and division at the G1/S and G2/M transitions, respectively. The cdc2-cyclin B kise is pivotal in regulating the G2/M transition. Cdc2 is phosphorylated at Thr14 and Tyr15 during G2-phase by the kises Wee1 and Myt1, rendering it ictive. The tumor suppressor protein retinoblastoma (Rb) controls progression through the late G1 restriction point (R) and is a major regulator of the G1/S transition. During early and mid G1-phase, Rb binds to and represses the transcription factor E2F. The phosphorylation of Rb late in G1-phase by CDKs induces Rb to dissociate from E2F, permitting the transcription of S-phase-promoting genes. In vitro, Rb can be phosphorylated at multiple sites by cdc2, cdk2, and cdk4/6. D damage triggers both the G2/M and the G1/S checkpoints. D damage activates the D-PK/ATM/ATR kises, which phosphorylate Chk at Ser345, Chk2 at Thr68 and p53. The Chk kises ictivate cdc25 via phosphorylation at Ser216, blocking the activation of cdc2.CDC20 binds to and activates the aphase-promoting complex (APC) during mitosis and G1 phase of the cell cycle. Moreover, CDC20 is necessary for ubiquitin ligase activity of the APC/cyclosome (APC/C). In metaphase MAD2L1 ictivates the CDC20-APC/C complex, while in aphase this inhibition is lost and CDC20-APC/C degrades its substrates. p53 and p21 suppress expression of CDC20 upon genotoxic stresses and ectopic introduction of p53. siR mediated knock-down of CDC20 in cancer cells leads to attenuated cell growth and induces G(2)/M arrest, suggesting that CDC20 is a possible therapeutic target of cancer. Organization of neurol circuits requires presyptic axol differentiatio.

General readings

1. Uzunova K  et al. APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex. Nat Struct Mol Biol 19:1116-23 (2012). WB ; Human .
2. Davoli T  et al. Persistent telomere damage induces bypass of mitosis and tetraploidy. Cell 141:81-93 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC20 antibody

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827] - Detection of Mouse CDC20 by Immunohistochemistry.  FFPE section of mouse teratoma.  Affinity purified rabbit anti-CDC20 used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit. Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

CDC20 antibody

CDC20 Mouse IgG1 AR12 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CDC20 antibody

CDC20 Mouse IgG1 AR12 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDC20 antibody

CDC20 Mouse IgG1 AR12 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CDC20 antibody

Western blot analysis of extracts from HeLa cells and Jurkat cells, using CDC20 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC20 antibody

Predicted band size: 55kDa. Positive control: Human cervical cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/600 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC20 antibody

Western blot analysis of 293T cell and Human cervical cancer tissue using TA321318(CDC20 Antibody) at dilution 1/600 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC20 antibody

Western blot analysis of 293 cell lysate using CDC20 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC20 antibody

WB Suggested Anti-CDC20 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysateCDC20 is supported by BioGPS gene expression data to be expressed in HeLa Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC20 antibody

Western blot (WB) analysis of Cdc20 antibody (Cat.-No.: AP06783PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:Rat liver tissue lysate Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC20 (C-term) antibody

Figure 1. Conjugation pathways for ubiquitin and
ubiquitin-like modifiers (UBLs). Most modifiers mature by
proteolytic processing from inactive precursors (a; amino acid).
Arrowheads point to the cleavage sites. Ubiquitin is expressed either
as polyubiquitin or as a fusion with ribosomal proteins. Conjugation
requires activating (E1) and conjugating (E2) enzymes that form
thiolesters (S) with the modifiers. Modification of cullins by RUB
involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in ubiquitination.
In contrast to ubiquitin, the UBLs do not seem to form multi-UBL
chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto-
tail. Whether HUB1 functions as a modifier is currently unclear.
APG12 and URM1 are distinct from the other modifiers because
they are unrelated in sequence to ubiquitin. Data contributed by
S.Jentsch, see references above. Rabbit Serum Hu E, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

CDC20 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and ANAPC11. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-ANAPC11 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDC20 antibody

Western blot analysis of extracts from Jurkat/HeLa cells, using p55CDC Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CDC20 antibody

Western blot analysis of extracts from Jurkat/HeLa cells, using p55CDC Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CDC20 antibody

Human Spleen; Spleen; CDC20 antibody - N-terminal region (AVARP03031_P050) in Human Spleen cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

CDC20 antibody

CDC20 Rabbit IgG Aff - Purified Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

CDC20 antibody

Western blot analysis of CDC20 expression in Raji (A); HEK293T (B); HL60 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Por WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CDC20 antibody

Western blot analysis of CDC20 expression in Raji (A); HEK293T (B); HL60 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Por WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CDC20 antibody

Western blot analysis of CDC20 expression in Raji (A); HEK293T (B); HL60 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Por WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CDC20 antibody

CDC20 Mouse IgG2a BA8 Purified Xen E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Items 1 to 20 of 28 total

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