CDC25A antibody

Principal name

CDC25A antibody

Alternative names for CDC25A antibody

CDC-25A, M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, Cell Division Cycle 25A

SwissProt ID

A7MBD1 (Bovin), P30304 (Human), P48964 (Mouse), P48965 (Rat)

Gene ID

993 (CDC25A), 171102 (Cdc25a)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Dot blot (Dot), ELISA (detection) (E(detection))

Background of CDC25A antibody

The cdc25 protein phosphatase family plays a critical role in activating cyclin-dependent kises (CDKs) via dephosphorylation of conserved Thr14/Tyr15 inhibitory phosphorylation sites. While cdc25C is primarily responsible for activating CDK1 to overcome the G2/M checkpoint and allow mitotic entry, the primary substrate of cdc25A is CDK2, which, when active, allows progression through the G1/S and intra-S checkpoints. Abundance, subcellular localization and activity of cdc25A is tightly controlled by a variety of mechanisms, including phosphorylation, ubiquitition, and inhibitory binding to 14-3-3 proteins. During normal cell cycle progression, elevated c-Myc and E2F transcription factor levels lead to increased cdc25A expression. When conditions are favorable for D synthesis, cdc25A and CDK2 form an activation loop, wherein each activates the other enzyme. D damage, on the other hand, leads to multisite phosphorylation at inhibitory sites (Ser123, Ser177, Ser278, Ser292, and Thr506) by Chk1 and Chk2, which result in 14-3-3 binding and ubiquitin-mediated degradation.

General readings

1. Min W  et al. Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation. Nat Commun 4:2993 (2013).
2. Giessrigl B  et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet 21:4615-27 (2012).
3. Vo NT  et al. Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells. Carcinogenesis 31:2074-81 (2010). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC25A antibody

  Rabbit Polyclonal Cdc25A Antibody  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDC25A Rabbit Polyclonal antibody. (TA590333) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A pSer76 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC25A (phospho S75) polyclonal antibody ( Cat # PAB0429 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

CDC25A antibody

  Rabbit anti-cdc25A (Phospho-Ser75) polyclonal antibody(Phospho-specific)  
Western blot analysis of extracts from A2780 cells using CDC25A antibody (Lanes 1 and 2) and CDC25A (phospho-Ser75) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit anti-CDC25A polyclonal antibody  
Western blot analysis of extracts from A2780 cells using CDC25A antibody (Lanes 1 and 2) and CDC25A (phospho-Ser75) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit polyclonal CDC25A (Ab-178) antibody  
Western blot analysis of extracts from Jurkat cells, using CDC25A (Ab-178) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit polyclonal CDC25A (Ser75) antibody(Phospho-specific)  
Western blot analysis of extracts from HuvEc, using CDC25A (Phospho-Ser75) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit polyclonal CDC25A (Thr507) antibody(Phospho-specific)  
Western blot analysis of extracts from HuvEc cells, using CDC25A (Phospho-Thr507) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit polyclonal CDC25A (Ser82) antibody(Phospho-specific)  
Western blot analysis of extracts from K562 cells and A549 cells, using CDC25A (Phospho-Ser82) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit polyclonal CDC25A (Ser178) antibody(Phospho-specific)  
Western blot analysis of extracts from A549 cells, treated with UV (15mins), using CDC25A (Phospho-Ser178) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Anti-CDC25A Rabbit Polyclonal Antibody  
Western blot analysis of Mouse heart tissue using TA323117(CDC25A Antibody) at dilution 1/162.5 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Anti-CDC25A Rabbit Polyclonal Antibody  
Predicted band size: 59 kDa . Positive control: Hela and 3T6 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: Hela and 3T6 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit Polyclonal CDC25A Antibody  
Western blot analysis of CDC25A expression in UV treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit Polyclonal CDC25A (Ser75) Antibody (Phospho-specific)  
Western blot analysis on HeLa cell lysate using Phospho-CDC25A(Ser75) Antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  CDC25A Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CDC25A antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit Polyclonal Phospho-CDC25A (Thr507) Antibody (Phospho-specific)  
CDC25A Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit Polyclonal anti-Cdc25A Phospho (Ser17) Antibody  
Hela cells were treated with 300 uM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs.  Nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody reactive against phosphorylated Cdc25A  (Ser17). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6207 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit anti-CDC25A Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CDC25A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  phospho-CDC25A-S76 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from Hela cells using phospho-CDC25A-S76 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit Polyclonal Anti-Phospho-CDC25A(Ser75) Antibody  
Western blot analysis on HeLa cell lysate using Phospho-CDC25A(Ser75) Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

  Rabbit Polyclonal Anti-CDC25A Antibody  
CDC25A Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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