CDC25A antibody

Principal name

CDC25A antibody

Alternative names for CDC25A antibody

CDC-25A, M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, Cell Division Cycle 25A

SwissProt ID

A7MBD1 (Bovin), P30304 (Human), P48964 (Mouse), P48965 (Rat)

Gene ID

993 (CDC25A)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Dot blot (Dot), ELISA (detection) (E(detection))

Background of CDC25A antibody

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

General readings

1. Min W  et al. Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation. Nat Commun 4:2993 (2013).
2. Giessrigl B  et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet 21:4615-27 (2012).
3. Vo NT  et al. Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells. Carcinogenesis 31:2074-81 (2010). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC25A antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDC25A Rabbit Polyclonal antibody. (TA590333) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A pSer76 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC25A (phospho S75) polyclonal antibody ( Cat # PAB0429 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

CDC25A antibody

Western blot analysis of extracts from A2780 cells using CDC25A antibody (Lanes 1 and 2) and CDC25A (phospho-Ser75) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Western blot analysis of extracts from Jurkat cells, using CDC25A (Ab-178) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Western blot analysis of Mouse heart tissue using TA323117(CDC25A Antibody) at dilution 1/162.5 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Predicted band size: 59 kDa . Positive control: Hela and 3T6 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: Hela and 3T6 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Western blot analysis of CDC25A expression in UV treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Western blot analysis of extracts of various cell lines, using CDC25A antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Hela cells were treated with 300 uM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs.  Nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody reactive against phosphorylated Cdc25A  (Ser17). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6207 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Western blot analysis of extracts of various cell lines, using CDC25A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25A antibody

Figure 1. AM05219AF-N CDC25A antibody staining of Paraffin Embedded Human Tonsil Section  (germinal center).
Figure 2. Western blot analysis of CDC25A expression in Hela cells (Lane 1), NIH/3T3 cells (Lane 2) and Rat-1 cells (Lane 3) using CDC25A antibody (AM05219AF-N). Mouse IgG2a DCS-121 Azide Free Hu, Ms, Rt IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDC25A antibody

CDC25A Mouse IgG2a DCS-121 Purified Hu IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

CDC25A antibody

Figure 1. Western blot analysis of CDC25A expression in Hela cells (A), NIH/3T3 cells (B-1) and Rat-1 cells (B-2) using CDC25A antibody (AM20026AF-N) Mouse IgG2a DCS-120 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDC25A antibody

Figure 2. Western blot analysis of CDC25A expression in Raji cells (Lane 1), NIH/3T3 cells (Lane 2) and Rat-1 cells (Lane 3) using CDC25A antibody (AM20027AF-N). Mouse IgG2a DCS-124 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDC25A pSer76 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using cdc25A (phospho-Ser75) antibody AP02425PU. Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

CDC25A pSer76 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using cdc25A (phospho-Ser75) antibody AP02425PU. Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

CDC25A antibody

Figure 1. Western blot analysis of extracts from A2780 cells using cdc25A antibody AP02684PU (Lane 1 and 2) and cdc25A (phospho-Ser75) antibody AP02425 (Lane 3 and 4). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

CDC25A antibody

Figure 1. Western blot analysis of extracts from A2780 cells using cdc25A antibody AP02684PU (Lane 1 and 2) and cdc25A (phospho-Ser75) antibody AP02425 (Lane 3 and 4). Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

CDC25A antibody

Western blot (WB) analysis of CDC25A in extracts from Jurkat cells using CDC25A antibody (Cat.-No. AP06379PU-N). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC25A pSer76 antibody

Immunohistochemistry Image: Testis Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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