CDC25B antibody

Principal name

CDC25B antibody

Alternative names for CDC25B antibody

CDC25HU2, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B

SwissProt ID

P30305 (Human), P30306 (Mouse), P30310 (Xenla), P48966 (Rat)

Gene ID

994 (CDC25B), 12531 (Cdc25b)

Available reactivities

Can (Canine), Hu (Human), Por (Porcine), Bov (Bovine), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection))

Background of CDC25B antibody

Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase , the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins . In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

General readings

J Biol Chem. 1997 Dec 26;272(52):32731-4.
Phosphorylation of human CDC25B phosphatase by CDK1-cyclin A triggers its proteasome-dependent degradation.
Baldin V, Cans C, Knibiehler M, Ducommun B.
Institut de Pharmacologie et de Biologie Structurale du CNRS, Universit

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC25B antibody

Western blot analysis of CDC25B expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25B antibody

Western blot analysis of CDC25B expression in UV treated Raw264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25B antibody

WB Suggested Anti-CDC25B Antibody Titration: 0.2-1 ug/ml; Positive Control: Daudi cell lysateCDC25B is supported by BioGPS gene expression data to be expressed in Daudi Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC25B antibody

WB Suggested Anti-CDC25B Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC25B antibody

Host: Rabbit; Target Name: Cdc25b; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC25B antibody

Western blot (WB) analysis of Cdc25B antibody (Cat.-No.: AP06043PU-N) at 1/500 dilution Lane1:Hela cell lysate Lane2:Jurkat cell lysate Lane3:Mouse kidney tissue lysate Lane4:Mouse brain tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC25B antibody

Immunohistochemistry (IHC) analyzes of CDC25B antibody (Cat.-No.: AP06509PU-N) in paraffin-embedded human breast tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC25B pSer187 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDC25B antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CDC25B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDC25B (N-term) antibody

Western blot analysis of Cdc25B Antibody (N-term)(AP13958PU-N) in A2058 cell line lysates (35 µg/lane). Cdc25B (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDC25B antibody

Western blot analysis of anti-CDC25B Antibody (AP13959PU-N) in Ramos cell line lysates (35 µg/lane). CDC25B (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

CDC25B pSer353 antibody

Western blot analysis of extracts from COS7 cells treated with Etoposide using Phospho-CDC25B (Ser353) antibody AP55901PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CDC25B pSer353 antibody

Western blot analysis of extracts from COS7 cells treated with Etoposide using Phospho-CDC25B (Ser353) antibody AP55901PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu WB
50 µl / €270.00
  Acris Antibodies GmbH

CDC25B antibody

CDC25B Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CDC25B antibody

Western Blot analysis of CDC25B expression in transfected 293T cell line (H00000994-T01) by CDC25B MaxPab polyclonal antibody.

Lane 1: CDC25B transfected lysate(65.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDC25B antibody

CDC25B Mouse IgG1 7E8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDC25B pSer187 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC25B (phospho S187) polyclonal antibody ( Cat # PAB0432 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

CDC25B antibody

Western blot analysis of CDC25B polyclonal antibody ( Cat # PAB2829 ) in Ramos cell line lysates ( 35 µg/lane ) . CDC25B ( arrow ) was detected using the purified polyclonal antibody. Rabbit Hu C, E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CDC25B pSer323 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30', using CDC25B (Phospho-Ser323) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CDC25B pSer323 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30', using CDC25B (Phospho-Ser323) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

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