CDC25C antibody

Principal name

CDC25C antibody

Alternative names for CDC25C antibody

M-phase inducer phosphatase 3,Dual specificity phosphatase Cdc25C,Cell Division Cycle 25C

SwissProt ID

A5D7P0 (Bovin), P30307 (Human), P30311 (Xenla), P48967 (Mouse), Q29029 (Pig)

Gene ID

995 (CDC25C)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of CDC25C antibody

The cdc25 family of proteins is composed of three members, cdc25A, cdc25B, and cdc25C. The different members activate the cyclin dependent kinases at different points in the cell cycle by dephosphorylating key residues. Cdc25C is a dual specific protein phosphatase that controls entry into mitosis by the dephosphorylation of cdk1 on Thr14 and Tyr15. Cdc25C activity is expressed predominantly in G2. Cdc25C selectively interacts with the cyclin B/cdk1 complex cytoplasmically and in fact is itself regulated by cyclin B/cdk1 in a positive feedback loop. Cdc25C itself exists as a phosphoprotein (56 kDa-59 kDa) that is phosphorylated on Ser216 throughout interphase but becomes dephosphorylated during mitosis. In the phosphorylated form, cdc25C binds a member of the 14-3-3 family of proteins, which constitutes a regulatory checkpoint for its activity. Cdc25C is phosphorylated by both prk and by a novel kinase, C-TAKI. In addition to regulation post-translationally by phosphorylation, the cdc25C gene is also p53 regulated, via a p53 response element in its promoter.

General readings

Bonnet,J., (2008) Biochem. Biophys. Res. Commun. 370 (3), 483-488

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC25C antibody

Western blot analysis of extracts of various cell lines , using CDC25C antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25C antibody

Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 ug/ml] Mouse IgG1 3B11 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDC25C antibody

Predicted band size: 53 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25C antibody

Predicted band size: 55 kDa . Positive control: HT29 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Treated with the peptide Lane2: HT29 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25C antibody

Western blot analysis of CDC25C expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC25C antibody

ELISA: Cdc25C Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC25C antibody

Immunohistochemical analysis of paraffin-embedded human Testis tissues using anti-CDC25C antibody Cat.-No AM06420SU-N Mouse IgG1 1F12 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CDC25C antibody

CDC25C Mouse IgG1 TC-14 Purified Hu ICC/IF, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDC25C antibody

CDC25C Mouse IgG1 TC-19 Purified Hu ICC/IF, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDC25C antibody

Figure 1. Western blot analysis of CDC25C expression in Hela cells (Lane 1), A-431 cells (Lane 2), ZR75-1 cells (Lane 3), HEp-2 cells (Lane 4), NIH/3T3 cells (Lane 5) and PC12 cells (Lane 6) using CDC25C antibody (AM20028AF-N) Mouse IgG1 DCS-193 Azide Free Hu, Ms, Rt WB
0.1 mg / €310.00
  Acris Antibodies GmbH

CDC25C antibody

CDC25C Mouse IgG1 IMD-25 Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDC25C pSer216 antibody

Immunohistochemistry (IHC) analysis of p-CDC25C (pSer216) antibody Cat.-No.: AP01548PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC25C pSer216 antibody

Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using cdc25c (pSer216) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

CDC25C antibody

Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using cdc25c (pSer216) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

CDC25C antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using cdc25C antibody AP02672PU. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

CDC25C antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using cdc25C antibody AP02672PU. Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

CDC25C antibody

Immunohistochemistry analysis of CDC25C Antibody (Cat.-No AP06044PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC25C (C-term) antibody

Western blot analysis of anti-CDC25C Antibody (C-term) in K562 cell line lysates (35ug/lane).CDC25C(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDC25C pSer198 antibody

CDC25C Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CDC25C pSer198 antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDC25C (Phospho-Ser198) antibody AP55808PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P
0.1 ml / €340.00
  Acris Antibodies GmbH

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