CDC27 antibody

Principal name

CDC27 antibody

Alternative names for CDC27 antibody

CDC27Hs, H-NUC, D0S1430E, D17S978E, Cell division cycle protein 27 homolog

SwissProt ID

P30260 (Human)

Gene ID

996 (CDC27)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Xen (Xenopus), Chk (Chicken), Chimp (Chimpanzee), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of CDC27 antibody

APC3 (anaphase-promoting complex subunit 3) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 95 kD. The APC3 protein is located at the spindle poles, the spindle microtubules, kinetochores and chromosome arms. This protein is required for core stability and functions as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC3 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The APC3 protein interacts with Rb, Mad2, p55CDC, BUBR1, as well as the APC complex proteins noted above. The APC3 protein can be modified by phosphorylation by cdk1-cyclin B.

General readings

General / background references:Gieffers C et al. Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons. Proc Natl Acad Sci U S A 96:11317-22 (1999). PubMed PMID: 10500174
Yamano H et al. The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor. EMBO J 17:5670-8 (1998). PubMed PMID: 9755167
Hershko A The ubiquitin pathway for protein degradation. Trends Biochem Sci 16:265-8 (1991). PubMed PMID: 1656558

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC27 antibody

Western blot analysis of extracts from HUVEC cells, HepG2 cells and COLO205 cells, using H-NUC antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC27 pThr244 antibody

WB using Anti-CDC27 pT244 antibody shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis.The primary antibody was diluted to 1:500. HRP Gt-a-Rabbit IgG [H&L] MX was used at 1:5000. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC27 (N-term) antibody

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against  APC3. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

CDC27 antibody

WB Suggested Anti-CDC27 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: NCI-H226 cell lysate.CDC27 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC27 antibody

Host: Rabbit; Target Name: CDC27; Sample Tissue: Fetal Liver lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC27 antibody

Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC27 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 antibody Cat.-No AM06470SU-N with DAB staining. Mouse IgG1 5C12 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CDC27 (C-term) antibody

Western blot anaylsis using AM20198AF-N CDC27 Antibody: CDC27 expression in Jurkat (Lane 1), Raji (Lane 2), K562 (Lane 3), HL-60 (Lane 4) and MCF-7 (Lane 5) cells. Mouse IgG2b AF3.1 Azide Free Bov, Can, Hu, Ms, Rt, Xen E, IP, P, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

CDC27 antibody

Western blot (WB) analysis of Cdc27 antibody (Cat.-No.: AP06780PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:HEK293T whole cell lysate Lane3:Rat kidney tissue lysate Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC27 pSer427 antibody

CDC27 Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Rt E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CDC27 (422-430) antibody

CDC27 Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Rt E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CDC27 (C-term) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CDC27 Control Peptide

CDC27
50 µg / €160.00
  Acris Antibodies GmbH

CDC27 antibody

CDC27 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

CDC27 antibody

Western blot using CDC27 polyclonal antibody ( Cat # PAB9986 ) shows detection of a band ~90 kDa corresponding to human CDC27 ( arrowhead ).
Approximately 35 µg of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1.0 µg/ml for 2 h atroom temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developedby LI-COR.
IRDye is a trademark of LI-COR, Inc. Chimp, Chk, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

CDC27 antibody

CDC27 Rabbit Chimp, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

CDC27 antibody

Western blot using CDC27 (phospho T244) polyclonal antibody ( Cat # PAB9988 ) shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 ( arrowhead ).
Lane 1 shows lysate from asynchronous cells.
Lane 2 shows lysate from cells treated with nocodazole.
Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis.
Each lane contains approximately 30 µg of HeLa whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose.
After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
ECL reagent was used for detection.
Data contributed by Bing Li, UT South western. Rabbit Bov, Chimp, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

CDC27 antibody

Western blot analysis of extracts from HuvEc/HepG2/COLO205 cells, using H-NUC Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CDC27 antibody

Western blot analysis of extracts from HuvEc/HepG2/COLO205 cells, using H-NUC Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CDC27 antibody

Human NCI-H226; WB Suggested Anti-CDC27 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: NCI-H226 cell lysate.. CDC27 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226.; CDC27 antibody - middle region (ARP43603_P050) in Human NCI-H226 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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