CDC34 antibody

Principal name

CDC34 antibody

Alternative names for CDC34 antibody

UBE2R1, E2-CDC34, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-protein ligase R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34

SwissProt ID

P49427 (Human), Q8CFI2 (Mouse)

Gene ID

997 (CDC34)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of CDC34 antibody

The Cdc 34 gene represents a mammalian homolog of the group of yeast Cdc 34 genes required for the late G1 - S phase transition. A key negative regulator of mitosis, the protein kinase Wee 1, is degraded in a Cdc 34 dependent fashion in Xenopus egg extracts. This proteolysis event is required for a timely entrance into mitosis and is inhibited when DNA replication is blocked. Therefore, the DNA replication checkpoint can prevent mitosis by suppressing the proteolysis of Wee 1 during S phase. The human Cdc 34 gene is located on the far telomeric region of 19p13. Mutants in the gene Cdc 34 of the yeast Saccharomyces cerevisiae are defective in the transition from G1 to the S phase of the cell cycle. This gene was cloned and shown to encode a 295 residue protein that has substantial sequence similarity to yeast Rad6 protein required for a variety of cellular functions including DNA repair and was recently shown to encode a ubiquitin conjugating enzyme. When produced in Escherichia coli, the Cdc 34 gene product catalyzes the covalent attachment of ubiquitin to histones H2A and H2B in vitro, demonstrating that the Cdc 34 protein is another distinct member of the family of ubiquitin conjugating enzymes. The cell cycle function of Cdc 34 is thus likely to be mediated by the ubiquitin conjugating activity of its product.

General readings

Sadowski M., et al. (2007) Biochem. J. 405:569-581.
Gazdoiu S., et al. (2005) Proc Natl Acad Sci USA 102(42):15053–8.

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC34 antibody

WB Suggested Anti-CDC34 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Thymus Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC34 antibody

Western blot analysis of extracts of various cell lines, using CDC34 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC34 antibody

CDC34 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDC34 antibody

Human Thymus; WB Suggested Anti-CDC34 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Thymus; CDC34 antibody - middle region (AVARP03048_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

CDC34 antibody

CDC34 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

CDC34 antibody

Western blot analysis of CDC34 expression in HL60 (A) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CDC34 antibody

Western blot analysis of CDC34 expression in HL60 (A) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CDC34 antibody

Western blot analysis of CDC34 expression in HL60 (A) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CDC34 antibody

Western Blot and  Immunoprecipitation: Cdc34 Antibody (90.12) [NB500-110] - Detection of human CDC34 protein in DU145 whole cell lysate. Also available in 200ug size Mouse IgG1 90.12 Purified Hu IP, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

CDC34 antibody

CDC34 Mouse IgG1 14B5 Purified Hu ICC/IF, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

CDC34 antibody

Western Blot:   Cdc34 Antibody [NBP1-32153]- (30µg whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:1000 Rabbit IgG Hu ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

CDC34 antibody

Immunohistochemistry-Paraffin: Cdc34 Antibody [NBP1-84946] - Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CDC34

CDC34 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CDC34 (1-236, His-tag)

Recombinant human CDC34, 1-236aa, His-tagged Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

CDC34 (1-236, His-tag)

Recombinant human CDC34, 1-236aa, His-tagged Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

CDC34

CDC34 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

CDC34

CDC34 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

CDC34

CDC34 Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

CDC34

CDC34 Human Purified
0.5 mg / €870.00
  Novus Biologicals Inc.

19 Item(s)

per page
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