CDC42 antibody

Principal name

CDC42 antibody

Alternative names for CDC42 antibody

Cell division control protein 42 homolog, G25K GTP-binding protein

SwissProt ID

P60766 (Mouse), P60952 (Canfa), P60953 (Human), Q007T2 (Pig), Q2KJ93 (Bovin), Q8CFN2 (Rat), Q90694 (Chick)

Gene ID

998 (CDC42), 64465 (Cdc42), 12540

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Por (Porcine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P)

Background of CDC42 antibody

CDC42 is a small GTPase of the Rho-subfamily, which regulates sigling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Altertive splicing of this gene results in multiple transcript variants.The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates sigling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Altertive splicing of this gene results in multiple transcript variants.

General readings

Kathryn P., et al. (2008) J Cell Biol. 183(6):1129-1143.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC42 (N-term) antibody

Western blot analysis of CDC42 in human brain tissue lysate with CDC42 antibody at (A) 0.5 and (B) 1 µg/ml. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC42 antibody

Western blot analysis of cdc42 in 3T3 (Lane 1) and Jurkat (Lane 2) cell lysate. Rabbit Purified Bov, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC42 antibody

Predicted band size: 23 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC42 antibody

Western blot analysis of extracts of various cell lines, using CDC42 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC42 antibody

WB Suggested Anti-CDC42 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysateCDC42 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC42 control peptide

CDC42
50 µg / €160.00
  Acris Antibodies GmbH

CDC42 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC42 and MAPK8 HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDC42 antibody

Immunocytochemical labeling in rat PC-12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Cdc42 monoclonal antibody, clone M152 ( Cat # MAB1376 ) was used at 1:50 dilution followed by labeling with donkey anti-mouse Cy2. Mouse IgG1 M152 Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

CDC42 antibody

CDC42 Rabbit Bov, Hu, Ms, Rt C, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

CDC42 antibody

Human 721_B; WB Suggested Anti-CDC42 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. CDC42 is supported by BioGPS gene expression data to be expressed in 721_B.; CDC42 antibody - N-terminal region (AVARP01017_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

CDC42 antibody

CDC42 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

CDC42 antibody

Western blot analysis of CDC42 expression in Jurkat (A); HT29 (B); mouse brain (C); rat liver (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Chk, Hu, Mky, Ms, Por, Rt, Ze WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CDC42 antibody

Western blot analysis of CDC42 expression in Jurkat (A); HT29 (B); mouse brain (C); rat liver (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Chk, Hu, Mky, Ms, Por, Rt, Ze WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CDC42 antibody

Western blot analysis of CDC42 expression in Jurkat (A); HT29 (B); mouse brain (C); rat liver (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Chk, Hu, Mky, Ms, Por, Rt, Ze WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

Cdc42 - mouse gene knockout kit via CRISPR

Cdc42 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cdc42 - mouse gene knockout kit via CRISPR

Cdc42 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cdc42 - mouse gene knockout kit via CRISPR

Cdc42 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cdc42 - mouse gene knockout kit via CRISPR

Cdc42 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CDC42 (transcript variant 1)

CDC42 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CDC42 (transcript variant 2)

CDC42 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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