CDC45L antibody

Principal name

CDC45L antibody

Alternative names for CDC45L antibody

CDC45-related protein, CDC45L2, PORC-PI-1, PORCPI1

SwissProt ID

O75419 (Human), Q9YHZ6 (Xenla), Q9Z1X9 (Mouse)

Gene ID

8318 (CDC45)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Fe (Feline), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)

Background of CDC45L antibody

CDC45L was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Hoskins,J.M., (2008) Clin. Cancer Res. 14 (6), 1788-1796

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC45L antibody

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. TA308625 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC45L antibody

WB Suggested Anti-CDC45 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Hela cell lysateCDC45 is supported by BioGPS gene expression data to be expressed in HeLa Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDC45L antibody

CDC45L Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDC45L antibody

CDC45L Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDC45L antibody

Immunoperoxidase of purified MaxPab antibody to CDC45L on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse Serum Hu E(detection), ICC/IF, P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

CDC45L antibody

CDC45L Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CDC45L antibody

CDC45L Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

CDC45L antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC45L and CDKN1A. HeLa cells were stained with anti-CDC45L rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDC45L antibody

Western Blot analysis of CDC45L expression in transfected 293T cell line by CDC45L monoclonal antibody (M01), clone 3F11-1F3.

Lane 1: CDC45L transfected lysate(65.6 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 3F11-1F3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDC45L antibody

CDC45L monoclonal antibody (M02), clone 4C2 Western Blot analysis of CDC45L expression in Jurkat ( Cat # L017V1 ). Mouse IgG2a 4C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDC45L antibody

Human HeLa; WB Suggested Anti-CDC45L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Hela cell lysate. CDC45 is supported by BioGPS gene expression data to be expressed in HeLa.; CDC45L antibody - C-terminal region (ARP51333_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CDC45L antibody

Immunofluorescence: CDC45L Antibody [NBP1-32772] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDC45L antibody at 1:200 dilution. Rabbit IgG Hu ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

CDC45L antibody

Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723] - Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CDC45L

CDC45L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CDC45L

CDC45L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CDC45L

CDC45L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

CDC45L

CDC45L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CDC45L

CDC45L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

CDC45L (Human) IP-WB Antibody Pair

CDC45L (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CDC45L 293T Cell Transient Overexpression Lysate(Denatured)

CDC45L 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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