CDC6 antibody

Principal name

CDC6 antibody

Alternative names for CDC6 antibody

CDC18L, CDC6-related protein, p62(cdc6), HsCDC6, Cdc18-related protein, HsCdc18, Cell division control protein 6 homolog

SwissProt ID

O89033 (Mouse), P09119 (Yeast), Q99741 (Human)

Gene ID

990 (CDC6), 990953 (Q8XHA9)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Ye (Yeast), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of CDC6 antibody

The cdc6 protein, originally described in budding yeast (cdc6p), is essential and limiting for DNA synthesis. The protein functions as a replication initiation protein and as such is involved in the early steps of replication in eukaryotes serving as a \clamp-loader\" for assembly of MCM proteins onto the replicating DNA. Recently the human homolog of the yeast cdc6 protein has been identified. The human cdc6 protein, p62cdc6, maps to chromosome 17q21.3 very close to the map position of the BRCA1 gene. Cdc6 is activated at the G1/S border by the activity of E2F3 and is expressed as a nuclear protein only in proliferating cells and not in quiescent cells. Recently cdc6 has been shown to interact specifically with the active cyclin A/cdk2 complex resulting in the phosphorylation of the cdc6 protein and a change in localization to the cytoplasmic compartment. Structurally, the cdc6 protein contains a cyclin dependent phosphorylation site, destruction boxes, a nucleotide binding/ATPase domain, and a potential leucine zipper suggesting an interaction with other proteins. Interestingly, the cdc6 protein has also been identified in a two-hybrid screen looking for PCNA interacting proteins."""

General readings

This clone has been used in:Perkins G et al. Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J 20:4836-45 (2001). PubMed PMID: 11532947
Donovan S et al. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci U S A 94:5611-6 (1997). PubMed PMID: 9159120

  1. Quick-Filters:
  2. Highly Validated (3)

Items 1 to 20 of 37 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC6 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CDC6 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808381)(1:150) Mouse IgG1 OTI1A4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDC6 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC6 and PCNA. Mahlavu cells were stained with anti-CDC6 rabbit purified polyclonal 1:1200 and anti-PCNA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDC6 pSer54 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC6 (phospho S54) polyclonal antibody ( Cat # PAB0435 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

CDC6 antibody

Staining of nuclear Jurkat lysate using CDC6 ANtibody Cat.-No AP32719PU-N at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDC6 antibody

Predicted band size: 63 kDa . Positive control: K562 and 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC6 antibody

Predicted band size: 63 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC6 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808380)(1:150) Mouse IgG1 OTI7B12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDC6 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6 (RC206814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDC6.(1:2000) Mouse IgG1 OTI1B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDC6 antibody

CDC6 Mouse IgG1 6-37 Purified Hu, Prim WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDC6 antibody

CDC6 Mouse IgG2a 6-36 Purified Hu, Prim, Rt IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDC6 antibody

CDC6 Mouse IgG1 IMD-6 Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDC6 antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CDC6 antibody (Cat.-No. AP06045PU-N). Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC6 antibody

Western blot (WB) analyzes of Cdc6 antibody (Cat.-No.: AP06778PU-N) in extracts from K562 cells.. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC6 (400-500) antibody

Testis: Formalin-Fixed Paraffin-Embedded Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µl / €470.00
  Acris Antibodies GmbH

CDC6 pSer54 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDC6 (N-term) antibody

Western blot analysis of Cdc6 Antibody (N-term) in Romas cell line lysates (35ug/lane). Cdc6 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDC6 antibody

CDC6 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDC6 antibody

CDC6 Mouse IgG1 IMD-6 Hu WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

CDC6 antibody

Western blot analysis of CDC6 polyclonal antibody ( Cat # PAB4853 ) in Romas cell lysate ( 35 µg/lane ) . CDC6 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CDC6 pSer54 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CDC6 (Phospho-Ser54) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

Items 1 to 20 of 37 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn