CDC7 antibody

Principal name

CDC7 antibody

Alternative names for CDC7 antibody

CDC7L1, CDC7-related kinase, HsCdc7, huCdc7, Cell division cycle 7-related protein kinase

SwissProt ID

O00311 (Human), P06243 (Yeast), Q9Z0H0 (Mouse)

Gene ID

8317 (CDC7)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ChIP assay (ChIP)

Background of CDC7 antibody

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. The involvement of the latter complex in DNA replication was shown by different assays such as injection of antibodies against Cdc7 kinase that caused inhibition of DNA replication. Furthermore, mutant mice lacking mouse Cdc7 kinase are early embryonic lethal at E3.5-6.5.1-3 Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The protein shares about 45% identity with yeast Cdc7 kinase in the kinase domain. Cdc7 kinase is inert as a kinase unless it is activated by the ASK protein (the human homologue of yeast Dbf4). The expression of ASK is tightly regulated with cell cycle. ASK protein level is very low at the M-G1 phase and increases at late G1, staying high during S phase and through G2 phase. As a consequence, the activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). The phosphorylation of proteins of the MCM complex by Cdc7 kinase is important for complex reorganization and duplex unwinding activity in the replication fork. Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

General readings

General / background references:Masai H & Arai K Cdc7 kinase complex: a key regulator in the initiation of DNA replication. J Cell Physiol 190:287-96 (2002). PubMed PMID: 11857444
Lei M & Tye BK Initiating DNA synthesis: from recruiting to activating the MCM complex. J Cell Sci 114:1447-54 (2001). PubMed PMID: 11282021
Jares P et al. The Cdc7/Dbf4 protein kinase: target of the S phase checkpoint? EMBO Rep 1:319-22 (2000). PubMed PMID: 11269496

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDC7 antibody

Western blot analysis of extracts from Jurkat cells, using CDC7 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDC7 antibody

Western blot analysis of extracts of various cell lines, using CDC7 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDC7 antibody

Anti-CDC7 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CDC7 antibody

Anti-CDC7 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Ms P
50 µg / €379.00
  OriGene Technologies, Inc.

CDC7 antibody

Figure 3. Immunohistochemical detection of CDC7 on Paraffin Embedded Section of Human Tonsil (germinal center) with CDC7 antibody (AM20025AF-N). Mouse IgG2b DCS-342 Azide Free Hu IP, P, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

CDC7 antibody

Western blot (WB) analysis of Cdc7 antibody (Cat.-No.: AP20508PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDC7 antibody

CDC7 Rabbit Purified Hu, Ms C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CDC7 antibody

Western blot analysis of extracts from Jurkat cells, using CDC7 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

CDC7 antibody

Western blot analysis of extracts from Jurkat cells, using CDC7 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

CDC7 antibody

CDC7 Mouse IgG1 DCS-341 Purified Hu E, ICC/IF, IP, WB
50 µl / €340.00
  Novus Biologicals Inc.

CDC7 antibody

Immunohistochemistry: CDC7 Antibody (SPM171) [NB120-17880] - IHC staining of human tonsil stained with anti-CDC7  antibody (NB120-17880) Mouse IgG1 SPM171 Purified Hu IP, P, WB
0.25 ml / €320.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CDC7

CDC7 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CDC7

CDC7 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

CDC7

CDC7 Human
5 µg / €610.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CDC7 overexpression lysate

CDC7 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CDC7 overexpression lysate

CDC7 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

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