CDCA8 / Borealin antibody

Principal name

CDCA8 / Borealin antibody

Alternative names for CDCA8 / Borealin antibody

Dasra-B, hDasra-B, PESCRG3, Cell division cycle-associated protein 8, Pluripotent embryonic stem cell-related gene 3 protein

SwissProt ID

Q53HL2 (Human), Q6AXW0 (Rat), Q8BHX3 (Mouse)

Gene ID

55143 (CDCA8), 52276 (Cdca8), 500545

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of CDCA8 / Borealin antibody

Encoded by gene CDCA8, borealin forms an important component of mitosis regulating chromosomal passenger complex (CPC complex - BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC) which is known for ensuring correct chromosome alignment/segregation and is required for chromatin-induced microtubule stabilization as well as spindle assembly. Borealin governs CPC to centromeric D and is a major effector of the TTK kise in the control of attachment-error-correction and chromosome alignment. Besides associating with CPC complex subunits, borealin interact with SENP3, UBE2I and RANBP2 which are involved its sumoylation. Borealin is expressed in a cell cycle-dependent manner and the levels of endogenous borealin significantly increase during mitosis. TTK can phosphorylated borealin at Thr-88, Thr94, Thr-169 and Thr-230 and and mutation of S219 to alanine in borealin causes defects in mitotic progression advocating for the importance of phosphorylation in borealin function. Mutation of additiol sites creates a form of borealin that is defective in localizing to the centromere, binding to Sgo1 and Sgo2, and in coorditing chromosome alignment.

General readings

Gassmann R., et al. (2004) J Cell Biol.166:179-191.
Chang J L., et al. (2006) EXP Cell Res. 312:962-973.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDCA8 / Borealin antibody

  CDCA8 mouse monoclonal antibody, clone OTI4G3 (formerly 4G3)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CDCA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807726)(1:150) Mouse IgG1 OTI4G3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDCA8 / Borealin antibody

  Rabbit Polyclonal CDCA8 Antibody  
Western blot analysis of CDCA8 in Rat kidney lysate with CDCA8 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDCA8 / Borealin antibody

  Rabbit Polyclonal Borealin Antibody  
Western Blot: Borealin Antibody [TA336746] - Mouse kidney lysate. Rabbit Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDCA8 / Borealin antibody

  Rabbit Polyclonal Anti-CDCA8 Antibody  
Gel: 15%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (CDCA8 Antibody) at dilution 1/900, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDCA8 / Borealin antibody

  CDCA8 mouse monoclonal antibody, clone OTI6A8 (formerly 6A8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA8 (RC201385, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCA8.(1:2000) Mouse IgG1 OTI6A8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDCA8 / Borealin antibody

  CDCA8 mouse monoclonal antibody, clone OTI5A6 (formerly 5A6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA8 (RC201385, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCA8.(1:2000) Mouse IgG2a OTI5A6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDCA8 / Borealin antibody

  CDCA8 mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA8 (RC201385, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCA8.(1:2000) Mouse IgG2a OTI2D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDCA8 / Borealin (1-280) antibody

Immunoflourescence detection of Borealin on acetone fixed HEp-2 cells with M147-3. Mouse IgG2a 1D11 Azide Free Hu ICC/IF, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

CDCA8 / Borealin antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 antibody (Center) (AP11424PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Cdca8 - mouse gene knockout kit via CRISPR

Cdca8 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cdca8 - mouse gene knockout kit via CRISPR

Cdca8 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cdca8 - mouse gene knockout kit via CRISPR

Cdca8 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cdca8 - mouse gene knockout kit via CRISPR

Cdca8 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CDCA8 / Borealin (full length, N-term HIS tag)

  Purified recombinant protein of Human cell division cycle associated 8 (CDCA8), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
CDCA8 / Borealin Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

CDCA8 / Borealin (19-280, His-tag)

CDCA8 / Borealin Human Purified > 90 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

CDCA8 / Borealin (19-280, His-tag)

CDCA8 / Borealin Human Purified > 90 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

CDCA8 overexpression lysate

CDCA8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Cdca8 (GFP-tagged) - Mouse cell division cycle associated 8 (Cdca8), 200 uL, >10^7 TU/mL

Lenti ORF particles, Cdca8 (GFP-tagged) - Mouse cell division cycle associated 8 (Cdca8), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Cdca8 (GFP-tagged) - Mouse cell division cycle associated 8 (cDNA clone MGC:92981 IMAGE:30634239), 200 uL, >10^7 TU/mL

Lenti ORF particles, Cdca8 (GFP-tagged) - Mouse cell division cycle associated 8 (cDNA clone MGC:92981 IMAGE:30634239), 200 uL, >10^7 TU/mL  
200 µl / €790.00
  OriGene Technologies, Inc.

Lenti ORF particles, Cdca8 (Myc-DDK-tagged) - Mouse cell division cycle associated 8 (Cdca8), 200 uL, >10^7 TU/mL

Lenti ORF particles, Cdca8 (Myc-DDK-tagged) - Mouse cell division cycle associated 8 (Cdca8), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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