CDK2 antibody

Principal name

CDK2 antibody

Alternative names for CDK2 antibody

p33 protein kinase, Cell division protein kinase 2,Cyclin dependent kinase 2

SwissProt ID

O55076 (Crigr), P23437 (Xenla), P24941 (Human), P97377 (Mouse), Q5E9Y0 (Bovin), Q63699 (Rat)

Gene ID

1017 (CDK2), 12566 (Cdk2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Por (Porcine), Sh (Sheep), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of CDK2 antibody

p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase. 
Distribution: Nuclear protein, predicted molecular weight 34 kD.
Function: Involved in the control of the cell cycle.
Interaction: Cyclins A, B1, B3, D, or E.

General readings

Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6.
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53.
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56.
Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK1 / CDK2 pThr14 antibody

Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) antibody AP55944PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CDK1 / CDK2 pThr14 antibody

Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) antibody AP55944PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI1C4 (formerly 1C4)  
Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (TA502807). Mouse IgG2a OTI1C4 Purified Hu, Mky F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)  
Anti-CDK2 mouse monoclonal antibody (TA502808) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494). Mouse IgG1 OTI2F10 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)  
Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (TA502832). Mouse IgG2a OTI1H5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI4B4 (formerly 4B4)  
Anti-CDK2 mouse monoclonal antibody (TA502833) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494). Mouse IgG2b OTI4B4 Purified Hu, Mky F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI4D3 (formerly 4D3)  
Anti-CDK2 mouse monoclonal antibody (TA502893) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494). Mouse IgG2a OTI4D3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI2A5 (formerly 2A5)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502915) Mouse IgG2a OTI2A5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502935) Mouse IgG2a OTI2D9 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI6H2 (formerly 6H2)  
Anti-CDK2 mouse monoclonal antibody (TA502936) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494). Mouse IgG2b OTI6H2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

  CDK2 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)  
Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (TA502943). Mouse IgG2a OTI1A8 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDK2 antibody

Immunohistochemical analysis of paraffin-embedded colon cancer (left) and cervical cancer (right) tissues using CDK2 antibody Cat.-No AM06741PU-N with DAB staining. Mouse IgG1 1A6 Purified Hu, Ms E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CDK2 antibody

  Rabbit polyclonal anti-CDK2 antibody  
Western blot analysis of extracts from HUVEC cells, HepG2 cells and Jurkat cells, using CDK2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK2 antibody

  Rabbit polyclonal CDK1/CDC2 (Thr14) antibody(Phospho-specific)  
Western blot analysis of extracts from HepG2 cells, treated with Forskolin (40nM, 30mins), using CDK1/CDC2 (Phospho-Thr14) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK2 antibody

  Rabbit polyclonal CDK2 (Ab-160) antibody  
Western blot analysis of extracts from A2780 cells, using CDK2 (Ab-160) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK2 antibody

  Rabbit polyclonal anti-cdk2 antibody  
Rabbit anti-cdk2 was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 ?g of lysate was separated on a 15% SDS-PAGE gel. Cdk-2 is indicated by an arrowhead as a 32-33 kDa band. Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Primary antibody was reacted at RT for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG  preceded color development. Rabbit Serum Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

CDK2 antibody

  Rabbit Polyclonal CDK1/CDC2 Antibody  
Western blot analysis of CDK1/CDC2 xpression in Forskolin treated HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK2 antibody

  Rabbit Polyclonal CDK2 Antibody  
Western blot analysis of CDK2 expression in A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK2 antibody

  Rabbit Polyclonal CDK1/CDC2 (Thr14) Antibody (Phospho-specific)  
Western blot analysis of CDK1/CDC2 phosphorylation expression in Forskolin treated HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK2 antibody

  Rabbit Polyclonal CDK2 (Thr160) Antibody (Phospho-specific)  
Western blot analysis of CDK2 phosphorylation expression in A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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