CDK4 antibody

Principal name

CDK4 antibody

Alternative names for CDK4 antibody

PSK-J3, Cell division protein kinase 4, Cyclin-dependent kinase 4

SwissProt ID

P11802 (Human), P30285 (Mouse), P35426 (Rat), P79432 (Pig), Q32KY4 (Bovin), Q91727 (Xenla)

Gene ID

1019 (CDK4), 12567 (Cdk4), 1019513 (Q8NQ97), 94201

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Ze (Zebrafish), Bov (Bovine)

Available hosts

Rabbit, Mouse, Chicken, Sheep

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of CDK4 antibody

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

General / background references:Elledge SJ Cell cycle checkpoints: preventing an identity crisis. Science 274:1664-72 (1996). PubMed PMID: 8939848
Lukas J et al. Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16. Nature 375:503-6 (1995). PubMed PMID: 7777060
Lukas J et al. Oncogenic aberrations of p16INK4/CDKN2 and cyclin D1 cooperate to deregulate G1 control. Cancer Res 55:4818-23 (1995). PubMed PMID: 7585513
Aagaard L et al. Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines. Int J Cancer 61:115-20 (1995). PubMed PMID: 7705923
Motokura T & Arnold A Cyclins and oncogenesis. Biochim Biophys Acta 1155:63-78 (1993). PubMed PMID: 8504131
Sherr CJ Mammalian G1 cyclins. Cell 73:1059-65 (1993). PubMed PMID: 8513492
Xiong Y et al. Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation. Genes Dev 7:1572-83 (1993). PubMed PMID: 8101826
Matsushime H et al. Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins. Cell 71:323-34 (1992). PubMed PMID: 1423597
Freeman RS & Donoghue DJ Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle. Biochemistry 30:2293-302 (1991). PubMed PMID: 2001361
Pines J & Hunter T Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport. J Cell Biol 115:1-17 (1991). PubMed PMID: 1717476
Yamashita M et al. Developmental Growth and Differentiation 33:617 (0).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK4 (C-term) antibody

Western blot analysis of anti-CDK4 Pab (Cat. #TA324944) in HL-60 cell lysate. CDK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

Western blot analysis of extracts of various cell lines, using CDK4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 4F11 Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK4 antibody

Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2G7 Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK4 antibody

CDK4 monoclonal antibody (M05), clone 2A1. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 2A1 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK4 antibody

CDK4 monoclonal antibody (M07), clone 8C3. Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 8C3 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK4 antibody

Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 8A10 Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK4 antibody

Immunohistochemistry: CDK4 Antibody [NBP1-31308] - Immunohistochemical analysis of paraffin-embedded clear cell OVCA , using CDK4 antibody at 1:100 dilution. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

CDK4 antibody

Predicted band size: 34kDa. Positive control: Hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lysates: 30 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

Western blot analysis of extracts from Hela cells, using anti-Cdk4, Poly6333. Rabbit IgG Poly6333 Purified Hu, Ms, Rt WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

Rat Cdk4 Antibody (C-term) (Cat. #TA328198) western blot analysis in rat lung tissue lysates (35ug/lane).This demonstrates the rat Cdk4 antibody detected the rat Cdk4 protein (arrow). Rabbit Purified Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

WB Suggested Anti-CDK4 Antibody Titration: 1.0ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

CDK4 Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

Western blot detection of CDK4 in Hela,Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa. Mouse IgG1 3F9-B12-C8 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

CDK4 Mouse IgG1 DCS-31.2 Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Mouse IgG2a IML-4 Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDK4 antibody

The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDK4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT6D10 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CDK4 antibody

The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDK4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT6D10 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Rabbit IgG Purified Hu IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

CDK4 (C-term) antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

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