CDK4 antibody

Principal name

CDK4 antibody

Alternative names for CDK4 antibody

PSK-J3, Cell division protein kinase 4, Cyclin-dependent kinase 4

SwissProt ID

P11802 (Human), P30285 (Mouse), P35426 (Rat), P79432 (Pig), Q32KY4 (Bovin), Q91727 (Xenla)

Gene ID

1019 (CDK4), 12567 (Cdk4), 94201

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CDK4 antibody

p34cdk4 is a major catalytic subunit of mammalian D-type cyclins which is expressed by a gene found in human chromosome 12q13. The cdk4 protein is constitutively expressed throughout the cell cycle and it is the assembly and accumulation of functional cdk4/cyclin D complexes which is rate limiting for the progression through the G1 phase of the cell cycle. Once the complex is formed it must be activated by the phosphorylation of threonine 172 on the cdk4 subunit, a reaction mediated by a cdk-activating kinase (CAK). Complex formation and phosphorylation are independently regulated events. The phosphorylation of the cdk4 subunit is subject to regulation of both stimulatory and inhibitory cytokine signals, thereby allowing cdk4 to function as a mid G1 checkpoint. Independent of phosphorylation, cdk4 is also regulated by the binding of p16Ink4/mts1 while in complex with cyclin D.

General readings

Coleman KG, et al. (1997). J. Biol. Chem. 272 (30): 18869–74.Li J, Melvin WS, Tsai MD, Muscarella P (2004). Biochemistry 43 (14): 4394–9.Dai K, Kobayashi R, Beach D (1996). J. Biol. Chem. 271 (36): 22030–4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK4 (C-term) antibody

  Rabbit polyclonal CDK4 Antibody (C-term)  
Western blot analysis of anti-CDK4 Pab (Cat. #TA324944) in HL-60 cell lysate. CDK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

  Rabbit anti-CDK4 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CDK4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

  Rabbit Polyclonal anti-Cdk4 Antibody  
Western blot analysis of extracts from Hela cells, using anti-Cdk4, Poly6333. Rabbit IgG Poly6333 Purified Hu, Ms, Rt WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

  Rabbit polyclonal anti-R Cdk4 antibody (C-term)  
Rat Cdk4 Antibody (C-term) (Cat. #TA328198) western blot analysis in rat lung tissue lysates (35ug/lane).This demonstrates the rat Cdk4 antibody detected the rat Cdk4 protein (arrow). Rabbit Purified Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

  Rabbit Polyclonal Anti-CDK4 Antibody  
WB Suggested Anti-CDK4 Antibody Titration: 1.0ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

  Rabbit Polyclonal Anti-CDK4 Antibody  
CDK4 Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

  Mouse Monoclonal CDK4 Antibody  
Western blot detection of CDK4 in Hela,Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa. Mouse IgG1 3F9-B12-C8 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK4 antibody

CDK4 Mouse IgG1 DCS-31.2 Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Mouse IgG2a IML-4 Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDK4 antibody

The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDK4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT6D10 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CDK4 antibody

The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDK4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT6D10 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Rabbit IgG Purified Hu IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CDK4 (C-term) antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Mouse IgG1 DCS-156 Purified Hu, Ms, Rt C, P, WB
50 µg / €250.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Mouse IgG1 DCS-35 Purified Hu, Ms, Por, Rt IP, WB
50 µg / €250.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Rabbit Serum Hu, Ms, Rt E, IP, WB
0.1 ml / €450.00
  Acris Antibodies GmbH

CDK4 antibody

CDK4 Mouse IgG1 DCS-31.2 Purified Hu, Ms, Rt C, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CDK4 - human gene knockout kit via CRISPR

CDK4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cdk4 - mouse gene knockout kit via CRISPR

Cdk4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cdk4 - mouse gene knockout kit via CRISPR

Cdk4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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