CDK7 antibody

Principal name

CDK7 antibody

Alternative names for CDK7 antibody

CAK1, P39 Mo15, STK1, CDK-activating kinase, Cell division protein kinase 7, TFIIH basal transcription factor complex kinase subunit, 39 kDa protein kinase

SwissProt ID

P20911, P50613 (Human), P51952 (Rat), P54685, Q03147 (Mouse)

Gene ID

399461 (cdk7), 1022 (CDK7), 12572 (Cdk7)

Available reactivities

Ze (Zebrafish), Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Whole mount Immunohistochemistry (IHC-wm), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of CDK7 antibody

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

General readings

Levedakou, E. N.; He, M.; Baptist, E. W.; Craven, R. J.; Cance, W. G.; Welcsh, P. L.; Simmons, A.; Naylor, S. L.; Leach, R. L.; Lewis, T. B.; Bowcock, A.; Liu, E. T. : Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern. Oncogene 9: 1977-1988, 1994.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK7 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDK7 and E2F1. Huh7 cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDK7 pThr170 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDK7 (phospho T170) polyclonal antibody ( Cat # PAB0439 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

CDK7 antibody

Western blot analysis of extracts from 293 cells, using CDK7 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK7 antibody

Predicted band size: 39 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK7 antibody

Untreated Hela cells (Lower Panel), or  overnight nocodazole treated Hela cells (Upper Panel) stained with purified rabbit polyclonal antibody against Ser167/Thr170 phosphorylated CAK, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI. Rabbit IgG Poly6324 Purified Hu ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK7 antibody

WB Suggested Anti-CDK7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDK7 antibody

WB Suggested Anti-CDK7 Antibody Titration: 0.6ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDK7 antibody

WB Suggested Anti-CDK7 Antibody Titration: 1ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK7 antibody

Western blot analysis on HeLa cell lysate using CDK7 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK7 (47-58) antibody

TA348965 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK7 antibody

CDK7 Mouse IgG2b MO1 Purified Hu C, IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

CDK7 antibody

CDK7 Mouse IgG2b MO-1.1 Aff - Purified C, F, ICC/IF, IP, WB
50 µg / €240.00
  Acris Antibodies GmbH

CDK7 antibody

CDK7 Mouse IgG2b IMD-26 Purified Hu C, ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDK7 antibody

Immunohistochemistry (IHC) analyzes of CDK7 antibody (Cat.-No.: AP06446PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDK7 antibody

Immunohistochemistry (IHC) analyzes of CDK7 antibody (Cat.-No.: AP06510PU-N) in paraffin-embedded human breast tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDK7 pSer167/pThr170 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu ICC/IF, P
50 µl / €470.00
  Acris Antibodies GmbH

CDK7 pThr170 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDK7 antibody

CDK7 polyclonal antibody (A01), Lot # 050726JC01 Western Blot analysis of CDK7 expression in Hela NE ( Cat # L013V3 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDK7 antibody

CDK7 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CDK7 antibody

CDK7 Mouse IgG2a 1G5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

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