CDK8 antibody

Principal name

CDK8 antibody

Alternative names for CDK8 antibody

Protein kinase K35, Cell division protein kinase 8, Cyclin-dependent kinase 8, Mediator of RNA polymerase II transcription subunit CDK8, Mediator complex subunit CDK8

SwissProt ID

P49336 (Human), Q66KH9 (Xenla), Q8R3L8 (Mouse)

Gene ID

1024 (CDK8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of CDK8 antibody

Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression.Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2–Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Large complexes containing Cdk8, cyclin C and the large subunit of RNA polymerase II have been identified. Cdk8 is thought to regulate RNA polymerase II function in conjunction with cyclin C. Cdk8 has been demonstrated to function as a transcriptional activator when fused to the DNA binding domain of GAL4.

General readings

Akoulitchev,S., et al., (2000) Nature 407 (6800), 102-106

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK8 antibody

CDK8 monoclonal antibody (M01), clone 6H5. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 6H5 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK8 antibody

CDK8 monoclonal antibody (M02), clone 6E5. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 6E5 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK8 antibody

CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 5H4 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK8 antibody

CDK8 monoclonal antibody (M04), clone 2E6. Western Blot analysis of CDK8 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 2E6 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK8 antibody

WB Suggested Anti-CDK8 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK8 antibody

Western blot (WB) analysis of CDK8 antibody (Cat.-No.: AP06697PU-N) at 1/500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDK8 (N-term) antibody

Western blot analysis of CDK8 antibody (N-term) in Jurkat cell line lysates (35ug/lane). CDK8 (arrow) was detected using the purified Pab Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDK8 antibody

CDK8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDK8 antibody

Western blot analysis of CDK8 polyclonal antibody ( Cat # PAB2918 ) in HL-60 cell lysate. CDK8 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CDK8 antibody

Western blot analysis of extracts from 293 cells, using CDK8 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

CDK8 antibody

Western blot analysis of extracts from 293 cells, using CDK8 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

CDK8 antibody

Human HepG2; WB Suggested Anti-CDK8 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; CDK8 antibody - C-terminal region (AVARP03050_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

CDK8 antibody

Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CDK8 antibody

Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CDK8 antibody

Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CDK8 antibody

CDK8 Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

CDK8 antibody

CDK8 Rabbit IgG Aff - Purified Hu, Ms E, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CDK8

CDK8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CDK8

CDK8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CDK8

CDK8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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