CDK9 antibody

Principal name

CDK9 antibody

Alternative names for CDK9 antibody

PITALRE, CDC2L4, Cell division protein kinase 9, Cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, Cell division cycle 2-like protein kinase 4

SwissProt ID

P50750 (Human), Q5EAB2 (Bovin), Q5ZKN1 (Chick), Q641Z4 (Rat), Q99J95 (Mouse)

Gene ID

1025 (CDK9), 107951 (Cdk9), 1025304 (gnd)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of CDK9 antibody

Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation.

General readings

Graña, X., DeLuca, A., Sang, N., Fu, Y., Claudio, P., Rosenblatt, J., Morgan, D. and Giordano, A. (1994) PITALRE a nuclear cdc2-related protein kinase that phosphorylates the retinoblastoma protein in vitro. Proc. Natl. Acd Sci. USA 91; 3834-3838.
Bullrich, F., MacLachlan, T., Sang, N., Druck, T., Veronese, M.L., Allen, S., Chirazzi, N., Koff, A., Heubner, K., Croce, C. and Giordano, A. (1995) Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, PITALRE and a cdk inhibitor, p27, to regions involved in human cancer. Cancer Res. 55; 1199-1205.
Bagella, L., MacLachlan, T.K., Buono, R.J., Pisano, M.M., Giordano, A. and De Luca, A. (1998) Cloning of murine cdk9/PITALRE and its tissue-specific expression in development. J. Cell Physiol. 177(2):206-13.
MacLachlan, T.K., Sang, N., De Luca, A.., Puri, P.L., Levrero, M. and Giordano, A. and (1998) Binding of cdk9 to TRAF2. J. Cell Biochem. 71(4):467-478.
De Falco, G. and Giordano, A. (1998) CDK( (PITALRE): A Multifunctional cdc-2-related Kinase. J. Cell. Physiol. 177; 501-506.
Fujinaga, K., Cujec, T.P., Peng, J., Garriga, J., Price, D.H., Graña, X. and Peterlin, B.M. (1998) The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1 and Tat. J. Virol. 72(9) 7154-7159.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK9 antibody

Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using CDK9 mouse mAb with DAB staining. Mouse IgG1 1B5A7 Ascites Hu E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CDK9 antibody

anti cdk9 antibody (TA319415 1:1500 ) was used for Western blot analysis of 1) PC3 and 2) DU145 prostate cancer cells (50ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University Rabbit Serum Hu, Ms, Rt C, E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

Predicted band size: 53 kDa . Positive control: 823 and Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: 823 cell lysate Lane2: Hela cell lysate Lysates: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

WB Suggested Anti-CDK9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

WB Suggested Anti-CDK9 Antibody; Titration: 2.5 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

Western blot analysis of extracts of various cell lines, using CDK9 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and rectum cancer tissues (right) using CDK9 mouse mAb with DAB staining. Mouse IgG1 1B5D10 Ascites Hu E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CDK9 antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

CDK9 pThr29 antibody

Western blot using affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9  phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Rabbit IgG Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CDK9 antibody

CDK9 Rabbit Serum Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €520.00
  Acris Antibodies GmbH

CDK9 antibody

CDK9 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDK9 antibody

Western Blot analysis of CDK9 expression in transfected 293T cell line by CDK9 monoclonal antibody (M07), clone 2D7.

Lane 1: CDK9 transfected lysate(42.778 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2D7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK9 antibody

Immunocytochemical staining of mouse tissue using CDK9 polyclonal antibody ( Cat # PAB9941 ) .
The staining shows the location of CDK9 protein in developing mouse tissue. Arrows indicate areas of high expression.
Panel A : Peroxidase-DAB immunostaining of CDK9 protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle.
Similar staining is shown in Panel B in the dorsal root ganglia.
Panel C : Fluorescein immunofluorescence of CDK9 in skeletal muscle.
Similar staining is shown in Panel D in cardiac muscle. Hu, Ms, Rt
0.1 ml / €560.00
  Abnova Taiwan Corp.

CDK9 pThr29 antibody

Western blot using CDK9 (phospho T29) polyclonal antibody ( Cat # PAB10000 ) shows detection of phospho-CDK9.
100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29.
The primary antibody was used at a 1:1000 dilution.
Personal Communication, J. Brady, NCI, Bethesda, MD. Rabbit Hu, Ms, Rt E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

CDK9 pThr186 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with Forskolin 40nM 30', using CDK9 (Phospho-Thr186) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CDK9 pThr186 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with Forskolin 40nM 30', using CDK9 (Phospho-Thr186) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CDK9 antibody

Human 293T; WB Suggested Anti-CDK9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; CDK9 antibody - N-terminal region (AVARP03004_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

CDK9 antibody

Human 293T; WB Suggested Anti-CDK9 Antibody. . Titration: 2.5 ug/ml. . Positive Control: Transfected 293T; CDK9 antibody - N-terminal region (AVARP03004_T100) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

CDK9 antibody

Western blot analysis of CDK9 expression in HEK293T (A); SP2/0 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CDK9 antibody

Western blot analysis of CDK9 expression in HEK293T (A); SP2/0 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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