CDK9 antibody

Principal name

CDK9 antibody

Alternative names for CDK9 antibody

PITALRE, CDC2L4, Cell division protein kinase 9, Cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, Cell division cycle 2-like protein kinase 4

SwissProt ID

P50750 (Human), Q5EAB2 (Bovin), Q5ZKN1 (Chick), Q641Z4 (Rat), Q99J95 (Mouse)

Gene ID

1025 (CDK9), 107951 (Cdk9), 362110

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CDK9 antibody

CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2 and are known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase was also found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase IIdirected transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with, and is regulated by, its regulatory subunit, cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggests a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/CDK9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/CDK9(PITALRE) has been shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. CDK9(PITALRE) activity and cyclin T1 are essential for activation of viral transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein. CDK9 kinase activity is regulated by autophosphoryaltion of CDK9 at T29 and T186. T29 phosphorylation is uniquely induced by the HTLV-1 viral oncoprotein Tax.

General readings

Li,Q., (2005) J. Biol. Chem. 280 (31), 28819-28826

  1. Quick-Filters:
  2. Highly Validated (1)

Items 1 to 20 of 41 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK9 antibody

Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using CDK9 mouse mAb with DAB staining. Mouse IgG1 1B5A7 Ascites Hu E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CDK9 antibody

  Rabbit polyclonal anti-Cdk9 antibody  
anti cdk9 antibody (TA319415 1:1500 ) was used for Western blot analysis of 1) PC3 and 2) DU145 prostate cancer cells (50ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University Rabbit Serum Hu, Ms, Rt C, E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

  Rabbit polyclonal CDK9 phospho T29 antibody (Phospho-specific)  
CDK9 Rabbit IgG Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

  Anti-CDK9 Rabbit Polyclonal Antibody  
Predicted band size: 53 kDa . Positive control: 823 and Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: 823 cell lysate Lane2: Hela cell lysate Lysates: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

  Rabbit Polyclonal Anti-CDK9 Antibody  
WB Suggested Anti-CDK9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

  Rabbit Polyclonal Anti-CDK9 Antibody  
WB Suggested Anti-CDK9 Antibody; Titration: 2.5 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

  Rabbit anti-CDK9 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CDK9 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDK9 antibody

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and rectum cancer tissues (right) using CDK9 mouse mAb with DAB staining. Mouse IgG1 1B5D10 Ascites Hu E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CDK9 antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

CDK9 antibody

CDK9 Rabbit Serum Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €520.00
  Acris Antibodies GmbH

CDK9 antibody

CDK9 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDK9 antibody

Western Blot analysis of CDK9 expression in transfected 293T cell line by CDK9 monoclonal antibody (M07), clone 2D7.

Lane 1: CDK9 transfected lysate(42.778 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2D7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDK9 antibody

Immunocytochemical staining of mouse tissue using CDK9 polyclonal antibody ( Cat # PAB9941 ) .
The staining shows the location of CDK9 protein in developing mouse tissue. Arrows indicate areas of high expression.
Panel A : Peroxidase-DAB immunostaining of CDK9 protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle.
Similar staining is shown in Panel B in the dorsal root ganglia.
Panel C : Fluorescein immunofluorescence of CDK9 in skeletal muscle.
Similar staining is shown in Panel D in cardiac muscle. Hu, Ms, Rt
0.1 ml / €560.00
  Abnova Taiwan Corp.

CDK9 pThr29 antibody

Western blot using CDK9 (phospho T29) polyclonal antibody ( Cat # PAB10000 ) shows detection of phospho-CDK9.
100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29.
The primary antibody was used at a 1:1000 dilution.
Personal Communication, J. Brady, NCI, Bethesda, MD. Rabbit Hu, Ms, Rt E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

CDK9 pThr186 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with Forskolin 40nM 30', using CDK9 (Phospho-Thr186) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CDK9 pThr186 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with Forskolin 40nM 30', using CDK9 (Phospho-Thr186) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CDK9 antibody

Human 293T; WB Suggested Anti-CDK9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; CDK9 antibody - N-terminal region (AVARP03004_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

CDK9 antibody

Human 293T; WB Suggested Anti-CDK9 Antibody. . Titration: 2.5 ug/ml. . Positive Control: Transfected 293T; CDK9 antibody - N-terminal region (AVARP03004_T100) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

CDK9 antibody

Western blot analysis of CDK9 expression in HEK293T (A); SP2/0 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CDK9 antibody

Western blot analysis of CDK9 expression in HEK293T (A); SP2/0 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Items 1 to 20 of 41 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn