CDKN1A / p21WAF1 antibody

Principal name

CDKN1A / p21WAF1 antibody

Alternative names for CDKN1A / p21WAF1 antibody

CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1,CDK-interacting protein 1, Melanoma differentiation-associated protein 6

SwissProt ID

O19002 (Felca), P38936 (Human), P39689 (Mouse)

Gene ID

1026 (CDKN1A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Chimp (Chimpanzee), Bov (Bovine), Can (Canine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of CDKN1A / p21WAF1 antibody

The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

General readings

Erber R, Klein W, Andel T, Enders C, Born AI, Conradt C, Bartek J, Bosch FX. Aberrant p21CIP1/WAF1 protein accumulation in head-and-neck cancer. Int J Cancer (Pred. Oncol.). 74, 383-389 (1997)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDKN1A / p21WAF1 antibody

Formalin-Fixed, Paraffin-Embedded Human Bladder Carcinoma stained with p21 Antibody Cat.-No AM09100 (Clone WA-1). Mouse IgG1 WA-1 Purified Chimp, Hu, Mky, Ms, Rt F, ICC/IF, P
0.2 mg / €390.00
  Acris Antibodies GmbH

CDKN1A / p21WAF1 antibody

Formalin-Fixed, Paraffin-Embedded Human Bladder Carcinoma stained with p21 Antibody Cat.-No AM09100 (Clone WA-1). Mouse IgG1 WA-1 Purified Chimp, Hu, Mky, Ms, Rt F, ICC/IF, P
0.1 mg / €320.00
  Acris Antibodies GmbH

CDKN1A / p21WAF1 antibody

Formalin-Fixed, Paraffin-Embedded Human Bladder Carcinoma stained with p21 Antibody Cat.-No AM09100 (Clone WA-1). Mouse IgG1 WA-1 Purified Chimp, Hu, Mky, Ms, Rt F, ICC/IF, P
20 µg / €160.00
  Acris Antibodies GmbH

CDKN1A / p21WAF1 antibody

CDKN1A Antibody (C-term) (Cat. #TA324520) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow). Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808164)(1:150) Mouse IgG2b OTI7C8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808276)(1:150) Mouse IgG1 OTI4B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and CDKN1A. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2F1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDKN1A / p21WAF1 antibody

Western blot analysis of extracts from HeLa cells using p21Cip1 antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Mab anti-Human p21WAF1 antibody to detect human p21 by WB. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p21WAF1at RT for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. The antibody may be used to detect endogenous human p21WAF1. Mouse WA-1 Purified Hu E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Western blot analysis of LNCap cell using TA323670(CDKN1A Antibody) at dilution 1/370 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Western blot analysis of LNCap cell using TA323671(CDKN1A Antibody) at dilution 1/315 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Predicted band size: 18 kDa . Positive control: 293T cell, human brain malignant glioma and liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Human brain malignant glioma tissue lysate Lane3:Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Predicted band size: 18 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10+12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Western blot analysis of p21 Cip1 expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Western blot analysis of extracts of various cell lines, using CDKN1A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Immunohistochemistry staining of Paraffin Colon tissue by p21 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear) Mouse IgG2a DCS-60.2 Purified Hu P
1 ml / €510.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (RC201765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808128)(1:150) Mouse IgG1 OTI5G1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (RC201765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A.(1:500) Mouse IgG1 OTI6G2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (RC201765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A.(1:2000) Mouse IgG1 OTI2D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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