CDKN1A / p21WAF1 antibody

Principal name

CDKN1A / p21WAF1 antibody

Alternative names for CDKN1A / p21WAF1 antibody

CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1,CDK-interacting protein 1, Melanoma differentiation-associated protein 6

SwissProt ID

O19002 (Felca), P38936 (Human), P39689 (Mouse)

Gene ID

1026 (CDKN1A), 12575 (Cdkn1a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Mky (Monkey), Bov (Bovine), Can (Canine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)

Background of CDKN1A / p21WAF1 antibody

p21 (WAF1), a member of the cycle dependent kinase (CDK) inhibitor family, was first identified as a tumor suppressor or anti-oncogenic protein and negative regulator of the cell cycle. Over the years it became apparent that p21 has broad functions in regulating fundamental cellular programs including proliferation, differentiation, migration, senescence, and has both anti-oncogenic and oncogenic properties (reviewed in Romanov, 2012). Many antibody and other studies have demonstrated that p21 levels are frequently elevated in situations associated with reduced proliferation, including differentiation and senescence, and decreased in proliferative states. Subcellular localization may significant and nuclear antibody staining may be indicative of p21 functioning as a cell cycle inhibitor, tumor suppressor, or in a senescence program. In contrast, cytoplasmic antibody staining may indicate p21 is acting an oncogene through regulatation of migration, proliferation, or apoptosis.

General readings

Erber R, Klein W, Andel T, Enders C, Born AI, Conradt C, Bartek J, Bosch FX. Aberrant p21CIP1/WAF1 protein accumulation in head-and-neck cancer. Int J Cancer (Pred. Oncol.). 74, 383-389 (1997)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDKN1A / p21WAF1 antibody

Formalin-Fixed, Paraffin-Embedded Human Bladder Carcinoma stained with p21 Antibody Cat.-No AM09100 (Clone WA-1). Mouse IgG1 WA-1 Purified Chimp, Hu, Mky, Ms, Rt F, ICC/IF, P
0.2 mg / €410.00
  Acris Antibodies GmbH

CDKN1A / p21WAF1 antibody

Formalin-Fixed, Paraffin-Embedded Human Bladder Carcinoma stained with p21 Antibody Cat.-No AM09100 (Clone WA-1). Mouse IgG1 WA-1 Purified Chimp, Hu, Mky, Ms, Rt F, ICC/IF, P
0.1 mg / €320.00
  Acris Antibodies GmbH

CDKN1A / p21WAF1 antibody

Formalin-Fixed, Paraffin-Embedded Human Bladder Carcinoma stained with p21 Antibody Cat.-No AM09100 (Clone WA-1). Mouse IgG1 WA-1 Purified Chimp, Hu, Mky, Ms, Rt F, ICC/IF, P
20 µg / €180.00
  Acris Antibodies GmbH

CDKN1A / p21WAF1 antibody

  Rabbit polyclonal CDKN1A Antibody (C-term)  
CDKN1A Antibody (C-term) (Cat. #TA324520) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow). Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  CDKN1A mouse monoclonal antibody, clone OTI7C8 (formerly 7C8)  
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808164)(1:150) Mouse IgG2b OTI7C8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

  CDKN1A mouse monoclonal antibody, clone OTI4B11 (formerly 4B11)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808276)(1:150) Mouse IgG1 OTI4B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

  Rabbit anti-CDKN1A polyclonal antibody  
Western blot analysis of extracts from HeLa cells using p21Cip1 antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Mouse monoclonal p21 WAF1 antibody  
Mab anti-Human p21WAF1 antibody to detect human p21 by WB. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p21WAF1at RT for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. The antibody may be used to detect endogenous human p21WAF1. Mouse WA-1 Purified Hu E, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Anti-CDKN1A Rabbit Polyclonal Antibody  
Western blot analysis of LNCap cell using TA323670(CDKN1A Antibody) at dilution 1/370 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Anti-CDKN1A Rabbit Polyclonal Antibody  
Western blot analysis of LNCap cell using TA323671(CDKN1A Antibody) at dilution 1/315 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Anti-CDKN1A Rabbit Polyclonal Antibody  
Predicted band size: 18 kDa . Positive control: 293T cell, human brain malignant glioma and liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Human brain malignant glioma tissue lysate Lane3:Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Rabbit Polyclonal p21 Cip1 Antibody  
Western blot analysis of p21 Cip1 expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Rabbit Polyclonal p21 Cip1 (Thr145) Antibody (Phospho-specific)  
Western blot analysis of p21 Cip1 phosphorylation expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  CDKN1A Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CDKN1A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Mouse Monoclonal CDKN1A (p21) Antibody  
Immunohistochemistry staining of Paraffin Colon tissue by p21 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear) Mouse IgG2a DCS-60.2 Purified Hu P
1 ml / €600.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Phospho-CDKN1A-T145 Rabbit Polyclonal Antibody  
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-CDKN1A-T145 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  Rabbit Polyclonal p21 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (RC201765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1A / p21WAF1 antibody

  CDKN1A mouse monoclonal antibody, clone OTI5G1 (formerly 5G1)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808128)(1:150) Mouse IgG1 OTI5G1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

  CDKN1A mouse monoclonal antibody, clone OTI6G2 (formerly 6G2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (RC201765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A.(1:500) Mouse IgG1 OTI6G2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CDKN1A / p21WAF1 antibody

  CDKN1A mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (RC201765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A.(1:2000) Mouse IgG1 OTI2D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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