CDKN1B / KIP1 antibody

Principal name

CDKN1B / KIP1 antibody

Alternative names for CDKN1B / KIP1 antibody

p27Kip1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27

SwissProt ID

O19001 (Felca), P46414 (Mouse), P46527 (Human), Q6SLL5 (Canfa)

Gene ID

1027 (CDKN1B), 12576 (Cdkn1b)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Mky (Monkey), Bov (Bovine), Fe (Feline), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Dot blot (Dot), Flow Cytometry (F)

Background of CDKN1B / KIP1 antibody

Cyclin-dependent protein kinase inhibitors (CDKI) genes are considered the cell markers for cell senescence and DNA damage. Several of these inhibitors have characterized. The one of the cyclin dependent protein kinase inhibitor, also known as Kip, play an important role in cell cycle and apoptosis in a variety of cell types. There are two forms Kip cloned and are known to interact with cell division and cell cycle in various tissues including white blood cells, posterior regions of the lense and in the crypt cells of the gut. Two forms of Kip are cloned, P27Kip-1 and P57Kip-2 ( ). Over expression of P27(Kip1) arrests CHO-K1 derived cells in conditional G1-specfic phase which are useful for screening cytotoxic drugs for ability to discriminate proliferate cells from mitotically inactive ones (1). Induction of P27(Kip1) in b cell lymphomas promotes growth arrest and apoptosis by inhibiting the induction of FOXO3 by PI3K and by down regulation of suppressor cMyc (2). The cyclin-dependent kinase inhibitor p27 (Kip1) is an important cell cycle regulatory gene in breast cancer, and decreased p27 expression is associated with poor prognosis and is considered to be a mammary tumor suppressor gene (3).
In many human cancers, the cyclin-dependent kinase inhibitor p27(Kip1) is expressed at low or undetectable levels. The decreased p27(Kip1) expression allows cyclin-dependent kinase activity to cause cells to enter into S phase and correlates with poor patient survival. Inhibition of serine/threonine kinase Akt signaling by some pharmacological agents or by PTEN induces G(1) arrest, in part by up-regulating p27Kip1 (4). The Akt phosphorylates kip-1 at Ser 10; thr 187 and 198. P27Kip 1 is also phosphorylated by other kinase including protein kinase CK2which is composed of 2 catalytic subunits (alfa) and 2 regulatory subunits (beta) subunits. CK2 can phsophorylate many proteins that play important role in cell cycle. In the presence of regulatory subunit (beta subunit) the CK2 kinase can phosphorylate P27(kip1) at position serine 83 (5). Kip 1 is down regulated by MAP kinase reactivation in choroidal melanoma tumor-derived cell line (OCM-1) and this down-regulation is inhibited by MAP kinase inhibitor calpastatin (6).

General readings

General References:
1. Keller,U.B., Old,J.B., Dorsey,F.C., Nilsson,J.A., Nilsson,L., MacLean,K.H., Chung,L., Yang,C., Spruck,C., Boyd,K., Reed,S.I. and Cleveland,J.L. (2007) Myc targets Cks1 to provoke the suppression of p27Kip1, proliferation and lymphomagenesis. EMBO J. 26 (10), 2562-2574.
2. Naidu,R., Har,Y.C. and Taib,N.A. (2007) P27 V109G Polymorphism is associated with lymph node metastases but not with increased risk of breast cancer. J. Exp. Clin. Cancer Res. 26 (1), 133-140.
3. Pertia,A., Nikoleishvili,D., Trsintsadze,O., Gogokhia,N., Managadze,L. and Chkhotua,A. Prognostic significance of p27(Kip 1) expression in renal cell Carcinoma. Georgian Med News 143, 12-17.
4. Figueiredo,J.C., Knight,J.A., Cho,S., Savas,S., Onay,U.V., Briollais,L., Goodwin,P.J., McLaughlin,J.R., Andrulis,I.L. and Ozcelik,H. (2007) Polymorphisms cMyc-N11S and p27-V109G and breast cancer risk and prognosis. BMC Cancer 7, 99.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDKN1B / KIP1 antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 4B4-E6 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Immunoperoxidase of monoclonal antibody to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 5ug/ml] Mouse IgG1 4B4-E6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CDKN1B / KIP1 antibody

Formalin-Fixed, Paraffin-Embedded Human colon stained with CDKN1B / KIP1 Antibody Cat.-No AM10127PU (Clone SX53G8). Mouse IgG1 SX53G8 Purified Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Formalin-Fixed, Paraffin-Embedded Human colon stained with CDKN1B / KIP1 Antibody Cat.-No AM10127PU (Clone SX53G8). Mouse IgG1 SX53G8 Purified Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Formalin-Fixed, Paraffin-Embedded Human colon stained with CDKN1B / KIP1 Antibody Cat.-No AM10127PU (Clone SX53G8). Mouse IgG1 SX53G8 Purified Hu, Mky, Ms, Rt F, ICC/IF, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Formalin-Fixed, Paraffin-Embedded Human colon stained with CDKN1B / KIP1 Antibody Cat.-No AM33273PU (Clone SPM348). Mouse IgG1 SPM348 Purified Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Formalin-Fixed, Paraffin-Embedded Human colon stained with CDKN1B / KIP1 Antibody Cat.-No AM33273PU (Clone SPM348). Mouse IgG1 SPM348 Purified Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Formalin-Fixed, Paraffin-Embedded Human colon stained with CDKN1B / KIP1 Antibody Cat.-No AM33273PU (Clone SPM348). Mouse IgG1 SPM348 Purified Hu, Mky, Ms, Rt F, ICC/IF, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Human prostate stained with anti-p27Kip1 antibody Rabbit Purified Hu, Ms, Rt IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Human prostate stained with anti-p27Kip1 antibody Rabbit Purified Hu, Ms, Rt IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

CDKN1B / KIP1 antibody

Human prostate stained with anti-p27Kip1 antibody Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

CDKN1B / KIP1 (C-term) antibody

TA302712 (1µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

CDKN1B / KIP1 (C-term) antibody

TA302712 (1µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1B / KIP1 antibody

TA303087 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Fe, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1B / KIP1 antibody

Predicted band size: 22 kDa . Positive control: Lncap cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1B / KIP1 antibody

Western blot analysis of p27 Kip1 expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1B / KIP1 antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p27Kip1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6029 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

CDKN1B / KIP1 antibody

Western blot analysis of extracts of NIH3T3 cells, using CDKN1B antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1B / KIP1 antibody

Western blot detection of p27 Kip1 in 3T3 cell lysates using p27 Kip1 mouse mAb (1:1000 diluted).Predicted band size:27KDa.Observed band size:27KDa. Mouse IgG1 5B4-E6-E11-A8 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1B / KIP1 antibody

Western blot analysis using CDKN1B mAb against CDKN1B(AA: 1-198)-hIgGFc transfected HEK293 cell lysate. Mouse IgG1 3D8 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

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