CDKN1C / KIP2 antibody

Principal name

CDKN1C / KIP2 antibody

Alternative names for CDKN1C / KIP2 antibody

p57KIP2, Cyclin-dependent kinase inhibitor 1C,Cyclin-dependent kinase inhibitor p57

SwissProt ID

P49918 (Human), P49919 (Mouse)

Gene ID

1028 (CDKN1C), 12577 (Cdkn1c), 1027 (CDKN1B), 4747 (NEFL)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CDKN1C / KIP2 antibody

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kise inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway. p57 Kip2 (Cyclin-dependent kise inhibitor 1C) functions as a tumor suppressor. Mutations of p57 Kip2 have been associated with numerous human maligncies as well as Beckwith–Wiedemann syndrome (BWS), characterized by an increased risk of childhood cancer. The amino-termil CDK inhibitory domain, common to the family, binds to and inhibits CDK/cyclin complexes and restricts cell cycle progression. The unique central region of p57 Kip2 interactes with LIMK-1, a downstream effector of the Rho family of GTPases. By sequestering LIMK-1 in the nucleus, p57 Kip2 disrupts actin dymics within cells and may be linked to its essential role in embryonic development. In addition, the carboxyl-termil QT domain of p57KIP2 binds to and inhibits JNK/SAPK activity regulating cellular apoptosis and differentiation. Expression levels of human p57 Kip2 are more restricted then other CDK inhibitors and are controlled by ubiquitition/degradation via the Skp1/Cul1/F-box-type E3 ubiquitin ligase complex SCF-Skp2. This effect is dependent on Thr310. A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regulate protein stability.

General readings

General / background references:Hatada I et al. Genomic imprinting of human p57KIP2 and its reduced expression in Wilms' tumors. Hum Mol Genet 5:783-8 (1996). PubMed PMID: 8776593
Overall ML et al. p57K1P2 is expressed in Wilms' tumor with LOH of 11p15.5. Genes Chromosomes Cancer 17:56-9 (1996). PubMed PMID: 8889507

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDKN1C / KIP2 (N-term) antibody

AP23803PU P57 antibody staining of Human Hydatid Mole (Stromal Area). Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CDKN1C / KIP2 (N-term) antibody

AP23803PU P57 antibody staining of Human Hydatid Mole (Stromal Area). Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CDKN1C / KIP2 (N-term) antibody

AP23803PU P57 antibody staining of Human Hydatid Mole (Stromal Area). Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CDKN1C / KIP2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using p57KIP2 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1C / KIP2 (N-term) antibody

Western blot analysis of CDKN1C in 293 cell lysate with CDKN1C antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDKN1C / KIP2 antibody

Gel: 10%SDS-PAGE, Lysate: 40 µg, Lane: Hela cells, Primary antibody: CDKN1C Antibody at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1C / KIP2 antibody

Predicted cell location: Cytoplasm. Positive control: Human gastric and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CDKN1C antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CDKN1C / KIP2 antibody

CDKN1C Antibody (N-term) (Cat. #TA324373) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the CDKN1C antibody detected the CDKN1C protein (arrow). Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CDKN1C / KIP2 antibody

Immunohistochemistry staining of Paraffin Placenta tissue by p57 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear) Mouse IgG2b KP10 Purified Hu P
1 ml / €460.00
  OriGene Technologies, Inc.

CDKN1C / KIP2 antibody

Western blot analysis using CDKN1C antibody Cat.-No AM06544SU-N against HEK293 (1) and CDKN1C(AA: 214-316)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 3E3 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CDKN1C / KIP2 antibody

Formalin-Fixed, Paraffin-Embedded Human partial (let) and complete (right) moles stained with p57 antibody Cat.-No. AM10128SU-N using peroxidase conjugate and DAB chromogen. Note the nuclear staining of cytotrophoblasts in partial mole and negative in complete mole. Mouse IgG2b KP10 Supernatant Hu, Ms P
1 ml / €300.00
  Acris Antibodies GmbH

CDKN1C / KIP2 antibody

Figure 1. Western blot analysis of p57Kip2 expression in U937 cells (Lane 1), MCF-7 cells (Lane 2), ZR75-1 cells (Lane 3), HL60 cells (lane 4), NIH/3T3 cells (Lane 5) and PC12 cells (Lane 6) using CDKN1C/KIP2 antibody (AM20030AF-N). Mouse IgG1 DCS-230 Azide Free Hu WB
0.1 mg / €310.00
  Acris Antibodies GmbH

CDKN1C / KIP2 (N-term) Control Peptide

CDKN1C / KIP2
50 µg / €160.00
  Acris Antibodies GmbH

CDKN1C / KIP2 antibody

Formalin-fixed, paraffin-embedded human colon carcinoma stained with p57 Ab (Cat. DM291) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells. Mouse IgG2b 57P06 Purified Hu, Ms IP, P
1 ml / €670.00
  Acris Antibodies GmbH

CDKN1C / KIP2 antibody

Formalin-fixed, paraffin-embedded human colon carcinoma stained with p57 Ab (Cat. DM291) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells. Mouse IgG2b 57P06 Ig Fraction Hu, Ms P
0.5 ml / €430.00
  Acris Antibodies GmbH

CDKN1C / KIP2 antibody

CDKN1C / KIP2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CDKN1C / KIP2 pThr310 antibody

Immunohistochemistry analysis of paraffin-embedded human placenta, using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

CDKN1C / KIP2 pThr310 antibody

Immunohistochemistry analysis of paraffin-embedded human placenta, using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

CDKN1C / KIP2 antibody

Immunofluorescence analysis of HuvEc cells, using p57 Kip2 (Ab-310) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

CDKN1C / KIP2 antibody

Immunofluorescence analysis of HuvEc cells, using p57 Kip2 (Ab-310) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

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