CDT1 antibody

Principal name

CDT1 antibody

Alternative names for CDT1 antibody

DUB, RIS2, Double parked homolog, DNA replication factor Cdt1

SwissProt ID

Q8R4E9 (Mouse), Q9H211 (Human)

Gene ID

81620 (CDT1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CDT1 antibody

Geminin is a protein that inhibits DNA replication by preventing the loading of MCM complex on chromatin. Human CDT1 is a 65kDa protein that contains 546 amino acids and coimmunoprecipitates with Geminin. Inhibition of DNA replication by Geminin in cell-free cDNA replication extracts can be reversed by the addition of excess CDT1. In the normal cell cycle, CDT1 is present only in G1 and S phases, whereas Geminin is present in S and G2 phases. Wohlschlegel et al. (2000) concluded that their results suggest that Geminin inhibits inappropriate origin firing by targeting CDT1. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

General readings

Whittaker AJ et al. Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. Genes Dev 14:1765-76 (2000). PubMed PMID: 10898791
Wohlschlegel JA et al. Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. Science 290:2309-12 (2000). PubMed PMID: 11125146

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDT1 (C-term) antibody

TA302447 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CDT1 antibody

WB Suggested Anti-CDT1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ye P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDT1 antibody

CDT1 polyclonal antibody ( Cat # PAB6234 ) ( 0.05 µg/ml ) staining of human ovary lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CDT1 antibody

Mouse Brain; HC Suggested Anti-CDT1 antibody. Titration:2 ug/ml. Positive Control:Mouse brain stem cells; CDT1 antibody - C-terminal region (ARP46350_P050) in Mouse Brain cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CDT1 antibody

NB 100-1098 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

CDT1 antibody

Immunohistochemistry-Paraffin: CDT1 Antibody [NB100-2567] - FFPE section of human metastatic lymph node.  Detection: DAB staining using immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CDT1 antibody

Western Blot: CDT1 Antibody [NBP1-58114] - This Anti-CDT1 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CDT1

CDT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CDT1 overexpression lysate

CDT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

9 Item(s)

per page
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