CDT2 / DTL antibody

Principal name

CDT2 / DTL antibody

Alternative names for CDT2 / DTL antibody

Denticleless protein homolog, CDW1, DCAF2, L2DTL, RAMP, Lethal(2) denticleless protein homolog, Ddb1- and Cul4-associated factor 2, Retinoic acid-regulated nuclear matrix-associated protein, RA regulated Nuclear Matrix Associated Protein

SwissProt ID

Q3TLR7 (Mouse), Q5ZJW8 (Chick), Q9NZJ0 (Human)

Gene ID

51514 (DTL)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of CDT2 / DTL antibody

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.Tissue specificity: Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.

General readings

Centore, R.C., et al. Mol. Cell 40(1):22-33(2010)
Abbas, T., et al. Mol. Cell 40(1):9-21(2010)
Song, B., et al. Mol. Cancer 9, 96 (2010)
Li, J., et al. Br. J. Cancer 101(4):691-698(2009)
Abbas, T., et al. Genes Dev. 22(18):2496-2506(2008)

8 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDT2 / DTL antibody

WB Suggested Anti-DTL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysate.DTL is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDT2 / DTL (Center) antibody

Western blot analysis of CDT2 / DTL Antibody Cat.-No AP51328PU-N in mouse spleen tissue lysates (35ug/lane). This demonstrates the DTL antibody detected the DTL protein (arrow). Rabbit Ig Aff - Purified Hu, Ms F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CDT2 / DTL antibody

CDT2 / DTL Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CDT2 / DTL antibody

Human 721_B; WB Suggested Anti-DTL Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. DTL is supported by BioGPS gene expression data to be expressed in 721_B.; DTL antibody - N-terminal region (ARP46248_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CDT2 / DTL antibody

Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH. Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

CDT2 / DTL antibody

Western Blot: CDT2 Antibody [NBP1-54644] - This Anti-DTL antibody was used in Western Blot of 721_B tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

CDT2 / DTL antibody

Immunohistochemistry-Paraffin: CDT2 Antibody [NBP1-85283] - Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

DTL overexpression lysate

DTL overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

8 Item(s)

per page
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