CDT2 / DTL antibody

Principal name

CDT2 / DTL antibody

Alternative names for CDT2 / DTL antibody

Denticleless protein homolog, CDW1, DCAF2, L2DTL, RAMP, Lethal(2) denticleless protein homolog, Ddb1- and Cul4-associated factor 2, Retinoic acid-regulated nuclear matrix-associated protein, RA regulated Nuclear Matrix Associated Protein

SwissProt ID

Q3TLR7 (Mouse), Q5ZJW8 (Chick), Q9NZJ0 (Human)

Gene ID

51514 (DTL), 76843 (Dtl)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of CDT2 / DTL antibody

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

General readings

Centore, R.C., et al. Mol. Cell 40(1):22-33(2010)
Abbas, T., et al. Mol. Cell 40(1):9-21(2010)
Song, B., et al. Mol. Cancer 9, 96 (2010)
Li, J., et al. Br. J. Cancer 101(4):691-698(2009)
Abbas, T., et al. Genes Dev. 22(18):2496-2506(2008)

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDT2 / DTL antibody

  Rabbit Polyclonal Anti-DTL Antibody  
WB Suggested Anti-DTL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysate.DTL is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CDT2 / DTL (Center) antibody

Western blot analysis of CDT2 / DTL Antibody Cat.-No AP51328PU-N in mouse spleen tissue lysates (35ug/lane). This demonstrates the DTL antibody detected the DTL protein (arrow). Rabbit Ig Aff - Purified Hu, Ms F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

DTL - human gene knockout kit via CRISPR

DTL - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DTL - human gene knockout kit via CRISPR

DTL - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DTL - human gene knockout kit via CRISPR

DTL - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DTL - human gene knockout kit via CRISPR

DTL - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dtl - mouse gene knockout kit via CRISPR

Dtl - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dtl - mouse gene knockout kit via CRISPR

Dtl - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dtl - mouse gene knockout kit via CRISPR

Dtl - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dtl - mouse gene knockout kit via CRISPR

Dtl - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

DTL overexpression lysate

DTL overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DTL (mGFP-tagged) - Human denticleless homolog (Drosophila) (DTL), 200 uL, >10^7 TU/mL

Lenti ORF particles, DTL (mGFP-tagged) - Human denticleless homolog (Drosophila) (DTL), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, DTL (Myc-DDK tagged) - Human denticleless homolog (Drosophila) (DTL) , 200 uL, >10^7 TU/mL

Lenti ORF particles, DTL (Myc-DDK tagged) - Human denticleless homolog (Drosophila) (DTL) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Dtl (GFP-tagged) - Mouse denticleless homolog (Drosophila) (Dtl), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dtl (GFP-tagged) - Mouse denticleless homolog (Drosophila) (Dtl), 200 uL, >10^7 TU/mL  
200 µl / €1,430.00
  OriGene Technologies, Inc.

Lenti ORF particles, Dtl (Myc-DDK-tagged) - Mouse denticleless homolog (Drosophila) (Dtl), 200 uL, >10^7 TU/mL

Lenti ORF particles, Dtl (Myc-DDK-tagged) - Mouse denticleless homolog (Drosophila) (Dtl), 200 uL, >10^7 TU/mL  
200 µl / €1,280.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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