CEBPB antibody

Principal name

CEBPB antibody

Alternative names for CEBPB antibody

C/EBP beta, TCF5, LAP, NF-IL6, CCAAT/enhancer-binding protein beta, Liver activator protein, Nuclear factor NF-IL6, Transcription factor 5

SwissProt ID

O02755 (Bovin), P17676 (Human), P21272 (Rat), P28033 (Mouse), Q05826 (Chick)

Gene ID

12608 (Cebpb), 1051 (CEBPB), 24253

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), ChIP assay (ChIP), ELISA (detection) (E(detection))

Background of CEBPB antibody

C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBP β protein can be modified by phosphorylation and ubiquitination. 
Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients. 
Structure: C/EBP transcription factor family, basic leucine zipper, dimer. Isoforms C/EBPβ, LAP, LIP, approximately 35 kD, 32 kD, 20 kD, respectively. 
Distribution: Nuclear. 
Function: Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes.
Regulation: Growth hormoneModification:Phosphorylation, Ubiquitination. 
Interaction: CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ

General readings

1. Mir RA & Chauhan SS Down Regulation of a Matrix Degrading Cysteine Protease Cathepsin L, by Acetaldehyde: Role of C/EBPa. PLoS One 6:e20768 (2011). WB ; Human .
2. Younce CW  et al. MCP-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3T3-L1 pre-adipocytes without peroxisome proliferator-activated receptor gamma. J Biol Chem 284:27620-8 (2009). WB ; Mouse . 

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CEBPB (C/EBPB, LAP) antibody

Nuclear extracts from P388D1 (IL-1) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß (clone A16). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. As shown, this antibody specifically recognizes the LAP isoform of C/EBP ß. Mouse IgG1 A16 Purified Ms WB
0.1 mg / €310.00
  Acris Antibodies GmbH

CEBPB (C/EBPB, LAP) antibody

Nuclear extracts from P388D1 (IL-1) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß (clone A16). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. As shown, this antibody specifically recognizes the LAP isoform of C/EBP ß. Mouse IgG1 A16 Purified Ms WB
25 µg / €210.00
  Acris Antibodies GmbH

CEBPB antibody

P388D1 (IL-1) cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß Antibody (Clone 1H7). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG2a 1H7 Biotin Ms, Rt ChIP, ICC/IF, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

CEBPB antibody

P388D1 (IL-1) cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß Antibody (Clone 1H7). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG2a 1H7 Biotin Ms, Rt ChIP, ICC/IF, WB
25 µg / €260.00
  Acris Antibodies GmbH

CEBPB (68-81) antibody

AP32952PU-N (1 µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CEBPB antibody

Western blot analysis of extracts from Jurkat cells, using CEBPB antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CEBPB antibody

Western blot analysis of extracts from NIH/3T3 cells, using C/EBP-ß (Ab-235/188) antibody (#TA312911).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CEBPB antibody

Predicted band size: 36 kDa . Positive control: Rat brain, Mouse brain tissue and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Rat brain, Mouse brain tissue and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CEBPB antibody

Western blot analysis of C/EBP-beta expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CEBPB antibody

WB Suggested Anti-CEBPB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

CEBPB antibody

Western blot analysis on HepG2 cell lysate using CEBPB Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CEBPB (39-78) antibody

AM06039PU-N CEBPB Antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG 21A1 Azide Free Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CEBPB antibody

Western blot analysis using CEBPB mAb against human CEBPB (AA: 161-338) recombinant protein. (Expected MW is 44.5 kDa) Mouse IgG1 3H9 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CEBPB antibody

Immunohistochemistry (IHC) analyzes of C7EBP-beta antibody (Cat.-No.: AP06034PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CEBPB antibody

Western blot (WB) analysis of C/EBP-β antibody (Cat.-No.: AP06676PU-N) at 1/500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:H9C2 whole cell lysate Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CEBPB (C-term) antibody

Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (C-term) (AP17939PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2) . Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CEBPB antibody

Immunohistochemistry: SM6009 CEBPB  antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG1 47A1 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CEBPB antibody

Immunohistochemistry: SM6009 CEBPB  antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG1 47A1 Purified Hu, Ms E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

CEBPB antibody

CEBPB Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CEBPB antibody

Western Blot analysis of CEBPB expression in transfected 293T cell line (H00001051-T01) by CEBPB MaxPab polyclonal antibody.

Lane 1: CEBPB transfected lysate(37.95 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

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