CEBPD antibody

Principal name

CEBPD antibody

Alternative names for CEBPD antibody

C/EBP delta, NF-IL6-beta, CCAAT/enhancer-binding protein delta, Nuclear factor NF-IL6-beta

SwissProt ID

O02756 (Bovin), P49716 (Human), Q00322 (Mouse), Q03484 (Rat), Q9N0J3 (Sheep)

Gene ID

12609 (Cebpd), 1052 (CEBPD), 25695

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Sh (Sheep), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of CEBPD antibody

C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBP delta may increase binding activity, whereas phosphorylation of the alpha and beta family members may decrease binding affinity. C/EBP delta is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

General readings

Williams SC, Cantwell CA, Johnson PF, et al. A family of C/EBP-related proteins capable of forming covalently linked leucine zipper dimers in vitro. Genes Dev.1991; 5:1553-67.
Umek RM, Friedman AD, and McKnight SL. CCAAT-enhancer binding protein: a component of a differentiation switch. Science 1991; 251:288-92.
Ramji DP and Foka P. CCAAT/enhancer-binding proteins: structure, function and regulation. Biochem. J. 2002; 365:561-75.
Johnson PF, Landschulz WH, Graves BJ, et al. Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses. Genes Dev. 1987; 1:133-46.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CEBPD antibody

Western blot analysis of extracts from LOVO cells and RAW264.7 cells, using CEBPE antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CEBPD (N-term) antibody

Western Blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, ~35 kDa Rabbit IgG Aff - Purified Hu, Ms E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CEBPD (C-term) antibody

Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CEBPD antibody

Gel: 10%SDS-PAGE, Lysate: 40 µg, Lane: LO2 cells, Primary antibody: CEBPD Antibody at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CEBPD antibody

Gel: 10%SDS-PAGE, Lysate: 24 µg, Lane: Mouse lung tissue, Primary antibody: CEBPD Antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CEBPD (C-term) antibody

CEBPD Antibody (C-term) (Cat. #TA324499) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the CEBPD antibody detected the CEBPD protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CEBPD antibody

Rat Cebpd Antibody (Center) (Cat. #TA325023) western blot analysis in A549,Hela,NCI-H460,U-937,mouse NIH/3T3 cell line and rat lung,testis tissue lysates (35ug/lane).This demonstrates the Rat Cebpd antibody detected the Rat Cebpd protein (arrow). Rabbit IgG Purified Hu, Ms, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CEBPD antibody

WB Suggested Anti-CEBPD Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: COLO205 cell lysate; CEBPD is supported by BioGPS gene expression data to be expressed in COLO205 Rabbit IgG Purified Bov, GP, Hu, Ms, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

CEBPD (N-term) antibody

Western Blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, ~35 kDa Rabbit IgG Aff - Purified Hu, Ms E, IP, WB
25 µl / €190.00
  Acris Antibodies GmbH

CEBPD (N-term) antibody

CEBPD Antibody (N-term) (Cat. #AP50849PU-N) western blot analysis in mouse lung tissue lysates (35µg/lane).This demonstrates the CEBPD antibody detected the CEBPD protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CEBPD (C-term) Control Peptide

Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody Cat.-No. AP55534PU-N at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

CEBPD antibody

Western blot using Cebpd polyclonal antibody ( Cat # PAB10182 ) shows detection of endogenous protein in whole cell extracts from mouse primary macrophage cells.
The blot shows staining as follows : untreated wild-type ( WT ) cells ( lane 1 ), WT cells treated 3 h with LPS ( lane 2 ) and Cebpd knock-out cells treated 3H with LPS ( lane 3 ).
The band at ~35 kDa, indicated by the arrowhead, corresponds to endogenous Cebpd.
Primary antibody was used at 1 : 15,000.
ECL was used for detection.
Personal communication, E.Sterneck, NCI, Frederick, MD. Rabbit Hu, Ms E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

CEBPD antibody

Western blot analysis of extracts from LOVO/RAW264.7 cells, using CEBPD/E Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CEBPD antibody

Western blot analysis of extracts from LOVO/RAW264.7 cells, using CEBPD/E Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CEBPD antibody

Human COLO205; WB Suggested Anti-CEBPD Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate. CEBPD is supported by BioGPS gene expression data to be expressed in COLO205.; CEBPD antibody - middle region (ARP38582_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CEBPD antibody

Western Blot: CEBP Delta Antibody [NB110-85519] - Western blot using affinity purified anti-C/EBPd antibody (NB110-85519) shows detection of endogenous protein in whole cell extracts from mouse primary macrophage cells.  The blot shows staining as follows:  untreated wild-type (WT) cells (lane 1), WT cells treated 3 h with LPS (lane 2) and C/EBPd-knock-out cells treated 3H with LPS (lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to endogenous C/EBPd. Primary antibody was used at 1:15,000. Rabbit Aff - Purified Hu, Ms E, IP, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

CEBPD antibody

CEBPD Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

CEBPD antibody

CEBPD Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

C/EBP delta/epsilon antibody

Immunohistochemical analysis of C/EBP delta/epsilon staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

C/EBP delta/epsilon antibody

Immunohistochemical analysis of C/EBP delta/epsilon staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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