CENP-H antibody

Principal name

CENP-H antibody

Alternative names for CENP-H antibody

Centromere protein H, CENPH, ICEN35, Interphase centromere complex protein 35

SwissProt ID

Q3T0L1 (Bovin), Q90ZF9 (Chick), Q9H3R5 (Human), Q9QYM8 (Mouse)

Gene ID

64946 (CENPH), 26886 (Cenph)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of CENP-H antibody

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore coro, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore coro, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Saffery R., et al. (2003) Mol Cell. 12(2):509-16.
Orthaus S., et al. (2006) Biochem Biophys Res Commun. 15;348(1):36-46.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CENP-H antibody

  CENPH mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CENPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503872) Mouse IgG1 OTI2G4 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CENP-H antibody

  CENPH mouse monoclonal antibody, clone OTI2A11 (formerly 2A11)  
Anti-CENPH mouse monoclonal antibody (TA503912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CENPH(RC204531). Mouse IgG2b OTI2A11 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CENP-H antibody

  Rabbit Polyclonal Anti-CENPH Antibody - middle region  
WB Suggested Anti-CENPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

CENP-H antibody

  Rabbit Polyclonal Anti-CENPH Antibody - N-terminal region  
WB Suggested Anti-CENPH Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CENP-H antibody

  Rabbit Polyclonal Anti-CENPH Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: (CENPH Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CENP-H antibody

  CENPH mouse monoclonal antibody, clone OTI2E8 (formerly 2E8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (RC204531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH. Mouse IgG1 OTI2E8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CENP-H antibody

  CENPH mouse monoclonal antibody, clone OTI4G6 (formerly 4G6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (RC204531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH. Mouse IgG1 OTI4G6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CENP-H antibody

  CENPH mouse monoclonal antibody, clone OTI1D5 (formerly 1D5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (RC204531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH. Mouse IgG1 OTI1D5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CENP-H antibody

  CENPH mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (RC204531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH. Mouse IgG1 OTI1A6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CENP-H antibody

  CENPH mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (RC204531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH. Mouse IgG1 OTI1C2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CENP-H (Center) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with CENPH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CENPH - human gene knockout kit via CRISPR

CENPH - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CENPH - human gene knockout kit via CRISPR

CENPH - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CENPH - human gene knockout kit via CRISPR

CENPH - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CENPH - human gene knockout kit via CRISPR

CENPH - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cenph - mouse gene knockout kit via CRISPR

Cenph - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cenph - mouse gene knockout kit via CRISPR

Cenph - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cenph - mouse gene knockout kit via CRISPR

Cenph - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cenph - mouse gene knockout kit via CRISPR

Cenph - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CENP-H

  Recombinant protein of human centromere protein H (CENPH)  
CENP-H Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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