CENPE antibody
Principal name
CENPE antibody
Alternative names for CENPE antibody
Centromere-associated protein E, Centromeric protein E, CENP-E, Kinesin-related protein CENPE
Ncbi ID
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of CENPE antibody
- Kit Component:
- - KN224375G1, CENPE gRNA vector 1 in pCas-Guide vector
- KN224375G2, CENPE gRNA vector 2 in pCas-Guide vector
- KN224375D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence microscopy of CENPE monoclonal antibody, clone 1H12 ( Cat # MAB1924 ) was used to detect CENPE protein visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase ( panel A ) .Interphase cells show no discrete staining ( bottom left, panel B ) .HeLa cells were fixed in paraformaldehyde and stained using this primary antibody.AlexaFluor 555 conjugated anti-Mouse antibody ( red ) was used for detection.DNA was stained using bis-benzimide ( DAPI ) ( green ) .Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
Anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. AlexaFluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319576 | CENPE antibody |
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| Mouse monoclonal anti-CENP-E antibody | |||||||
 |
Mouse | 1H12 | Purified | Hu | E, ICC/IF, IP, WB |
0.1 mg /
€356.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351071 | CENPE antibody |
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| Rabbit Polyclonal Anti-CENPE Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN224375 | CENPE - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN224375BN | CENPE - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN224375LP | CENPE - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN224375RB | CENPE - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303127 | Cenpe - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN303127BN | Cenpe - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303127LP | Cenpe - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303127RB | Cenpe - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
