Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), Frozen Sections (C)
Western Blot: CEP128 Antibody [NBP1-78745]-Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-CEP128 antibody used for WB at 1 mcg/ml (A and B) and used for IP at 6 mcg/mg lysate. CEP128 was also immunoprecipitated by another rabbit anti-CEP128 antibody which recognizes an upstream epitope.
Formalin-fixed and paraffin-embedded human testis reacted with C14orf145 Antibody (Center) (GTX80638), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
C14orf145 Antibody (Center) (GTX80638) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.