CFLAR / Casper / I-FLICE antibody

Principal name

CFLAR / Casper / I-FLICE antibody

Alternative names for CFLAR / Casper / I-FLICE antibody

c-FLIP, CLARP, MRIT, CASH, FLAME-1, CASP8AP1, Usurpin, CASP8 and FADD-like apoptosis regulator, Caspase-eight-related protein, Inhibitor of FLICE, Cellular FLICE-like inhibitory protein, Caspase-like apoptosis regulatory protein, MACH-related inducer of toxicity, Caspase homolog, FADD-like antiapoptotic molecule 1

SwissProt ID

O15519 (Human), O35732 (Mouse), Q9UEW1 (Human)

Gene ID

8837 (CFLAR), 12633 (Cflar), 8797 (TNFRSF10A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), EPair for Elisa (EPair), Flow Cytometry (F)

Background of CFLAR / Casper / I-FLICE antibody

Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis that has various mes, such as c-FLIP, Casper, CLARP, FLAME, I-FLICE, MRIT, CASH, and Usurpin. FLIP is expressed as two altertive splice isoforms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) like those found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE), and contains an additiol death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both FLIP isoforms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some research studies have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease, resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siR or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkins lymphoma and ovarian and colon carcinomas.

General readings

Kim,T.W., et.al., Science 277 (5324), 373-376 (1997)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CFLAR / Casper / I-FLICE antibody

Immunohistochemistry (IHC) analysis of c-FLIP antibody (Cat.-No.: AP06808PU-N) in paraffin-embedded human colon carcinoma tissue Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2F12 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE (191-209) antibody

Human Lung (formalin-fixed, paraffin-embedded) stained with CFLAR antibody AP22849PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE antibody

Immunoperoxidase of monoclonal antibody to CFLAR on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Mouse IgG2a 2F12 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CFLAR / Casper / I-FLICE (C-term) antibody

Western blot analysis of FLIP in NIH/3T3 whole cell lysate with FLIP antibody at 1:500 dilution. Rabbit IgG Purified Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CFLAR / Casper / I-FLICE (N-term) antibody

Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CFLAR / Casper / I-FLICE (C-term) antibody

Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CFLAR / Casper / I-FLICE (C-term) antibody

Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CFLAR / Casper / I-FLICE antibody

Predicted band size: 25 kDa . Positive control: Human fetal kidney tissue lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CFLAR / Casper / I-FLICE antibody

Western blot analysis of extracts of Jurkat cell line, using CFLAR antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CFLAR / Casper / I-FLICE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFLAR (RC200608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFLAR. Mouse IgG1 OTI2D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CFLAR / Casper / I-FLICE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFLAR (RC200608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFLAR. Mouse IgG1 OTI2H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CFLAR / Casper / I-FLICE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFLAR (RC200608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFLAR. Mouse IgG3 OTI2B9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CFLAR / Casper / I-FLICE antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CFLAR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804581) Mouse IgG1 OTI2H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CFLAR / Casper / I-FLICE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFLAR (RC200608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFLAR. Mouse IgG2b OTI1B1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CFLAR / Casper / I-FLICE (1-376) antibody

Western blot analysis: Cell lysates of mouse kidney, MCF7 and rat spleen (40 µg) were resolved by SDS-PAGE, trans-ferred to NC membrane and probed with FLIP antibody Cat.-No. AM09055PU-N  (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 5D8 Purified Hu, Ms, Rt E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE (1-376) antibody

Western blot analysis: Cell lysates of mouse kidney, MCF7 and rat spleen (40 µg) were resolved by SDS-PAGE, trans-ferred to NC membrane and probed with FLIP antibody Cat.-No. AM09055PU-N  (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 5D8 Purified Hu, Ms, Rt E, WB
50 µl / €230.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE (Long Form) antibody

Western blot analysis of FLIP in HeLa (left), Jurkat (middle) and K562 (right) cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE (C-term) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti FLIP gamma/ delta (AAP05788PU-N) Rabbit IgG Purified Hu, Ms C, ICC/IF, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

CFLAR / Casper / I-FLICE (206-222) antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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