CHAC1 antibody
Principal name
CHAC1 antibody
Alternative names for CHAC1 antibody
Cation transport regulator-like protein 1
Ncbi ID
NP_077016, NP_077016.1, NP_001136248.1, NM_024111, NP_001136248, NP_081205.1, NM_026929, NM_001142776
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of CHAC1 antibody
- Kit Component:
- - KN303228G1, Chac1 gRNA vector 1 in pCas-Guide vector
- KN303228G2, Chac1 gRNA vector 2 in pCas-Guide vector
- KN303228D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Gerhard,D.S., Genome Res. 14 (10B), 2121-2127 (2004)
Human 721_B; WB Suggested Anti-CHAC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. CHAC1 is supported by BioGPS gene expression data to be expressed in 721_B.; CHAC1 antibody - C-terminal region (ARP42623_P050) in Human 721_B cells using Western Blot
Western blot analysis of CHAC1 expression in U2OS (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates.
Anti-CHAC1 mouse monoclonal antibody (TA507009) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAC1(RC200912).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1 (RC200912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAC1.
Anti-CHAC1 mouse monoclonal antibody (TA507010) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAC1(RC200912).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1 (RC200912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1 (RC200912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAC1.
Anti-CHAC1 mouse monoclonal antibody (TA507033) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAC1(RC200912).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1 (RC200912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1 (RC200912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAC1.
Anti-CHAC1 mouse monoclonal antibody (TA507049) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAC1(RC200912).
Immunofluorescent staining of HeLa cells using anti-CHAC1 mouse monoclonal antibody (TA507049).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1 (RC200912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAC1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CHAC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507052)
Anti-CHAC1 mouse monoclonal antibody (TA507052) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAC1(RC200912).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAC1 (RC200912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAC1.
WB Suggested Anti-CHAC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateCHAC1 is supported by BioGPS gene expression data to be expressed in 721_B
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA507052 | CHAC1 antibody |
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| CHAC1 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2) | |||||||
 |
Mouse | IgG1 | OTI1E2 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
|
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| TA329470 | CHAC1 antibody |
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| Rabbit Polyclonal anti-CHAC1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA507009 | CHAC1 antibody |
||||||
| CHAC1 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2) | |||||||
 |
Mouse | IgG1 | OTI1G2 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA507010 | CHAC1 antibody |
||||||
| CHAC1 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1) | |||||||
 |
Mouse | IgG1 | OTI1G1 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA507032 | CHAC1 antibody |
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| CHAC1 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3) | |||||||
 |
Mouse | IgG1 | OTI1E3 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA507033 | CHAC1 antibody |
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| CHAC1 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7) | |||||||
 |
Mouse | IgG1 | OTI1G7 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA507048 | CHAC1 antibody |
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| CHAC1 mouse monoclonal antibody, clone OTI4B3 (formerly 4B3) | |||||||
 |
Mouse | IgG1 | OTI4B3 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA507049 | CHAC1 antibody |
||||||
| CHAC1 mouse monoclonal antibody, clone OTI4A11 (formerly 4A11) | |||||||
 |
Mouse | IgG1 | OTI4A11 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200912 | CHAC1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200912BN | CHAC1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200912LP | CHAC1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200912RB | CHAC1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303228 | Chac1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303228BN | Chac1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303228LP | Chac1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN303228RB | Chac1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300912 | CHAC1 (transcript variant 1) |
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| Recombinant protein of human ChaC, cation transport regulator homolog 1 (E. coli) (CHAC1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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| TP327708 | CHAC1 (transcript variant 2) |
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| Purified recombinant protein of Homo sapiens ChaC, cation transport regulator homolog 1 (E. coli) (CHAC1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411345 | CHAC1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY432159 | CHAC1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
