CHAT antibody

Principal name

CHAT antibody

Alternative names for CHAT antibody

Choline O-acetyltransferase, Choline acetylase, CHOACTase, ChAT, EC=2.3.1.6

SwissProt ID

P13222 (Pig), P28329 (Human), P32738 (Rat), Q03059 (Mouse), Q90YJ9 (Chick)

Gene ID

1103 (CHAT), 396896

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), GP (Guinea Pig), Rb (Rabbit), Chk (Chicken), Por (Porcine), Ze (Zebrafish), Can (Canine), Chimp (Chimpanzee), Av (Birds)

Available hosts

Chicken, Mouse, Goat, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoelectron Microscopy (EM)

Background of CHAT antibody

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

General readings

Crawford, G.D. et al., (1982) PNAS USA 79: 7031-7035.
Lindh, B. et al., (1986) PNAS USA 83: 5316-5320.
Matthws, D.A. et al., (1987) Brain Res. 402: 30-43.
Hagg, T. et al., (1992) Neuron 8: 145-158.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHAT (C-term) antibody

TA302990 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Chimp, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHAT (C-term) antibody

TA305830 (0.2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Western blot of rat brain lysate showing specific immunolabeling of the ~70k ChAT Goat IgG Aff - Purified Av, Chk, GP, Hu, Ms, non-Human Primates, Rt C, EM, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Western blot of rat brain lysate showing specific immunolabeling of the ~68/70k ChAT Rabbit IgG Serum Av, Chk, GP, Hu, Ms, non-Human Primates, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Western blot analysis of extracts of various cell lines, using CHAT antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Immunofluorescence using ChAT antibody Cat.-No AP31801PU-N: A tissue Section throught an adult Mouse brain showing ChAT (Red staining) in cholinergic neurons of the caudate-putamen nucleus. The pale green staining is autofluorescence of Green Fluorescent Protein (GFP) in this transgenic animal. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P
0.1 mg / €500.00
  Acris Antibodies GmbH

CHAT (N-term) antibody

Confocal immunofluorescent analysis of CHAT Antibody (N-term) (Cat. #AP50885PU-N) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu, Ms ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CHAT antibody

CHAT Mouse IgG1 1E6 Ascites Hu, Rt C
0.1 ml / €490.00
  Acris Antibodies GmbH

CHAT antibody

CHAT Goat Serum GP, Hu, Ms, Rt C, WB
50 µl / €520.00
  Acris Antibodies GmbH

CHAT antibody

CHAT Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

CHAT antibody

CHAT Mouse IgG1 1H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CHAT antibody

CHAT polyclonal antibody ( Cat # PAB6736 ) ( 0.03 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CHAT antibody

The Chat polyclonal antibody ( Cat # PAB7033 ) ( 0.2 µg/ml ) staining of mouse brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CHAT antibody

Immunofluorescent staining of rat basal forebrain cholinergic neurons using CHAT polyclonal antibody ( Cat # PAB14313 ). Rabbit GP, Ms, Rb, Rt C, E, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

CHAT antibody

Western blot analysis of CHAT expression in IMR32 (A); SKNSH (B) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt, Ze WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CHAT antibody

Western blot analysis of CHAT expression in IMR32 (A); SKNSH (B) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt, Ze WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CHAT antibody

Western blot analysis of CHAT expression in IMR32 (A); SKNSH (B) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt, Ze WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CHAT antibody

A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. Chicken Aff - Purified Hu, Ms C, ICC/IF, WB
0.1 ml / €180.00
  Neuromics Antibodies

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CHAT

CHAT Rat E. coli
10 µg / €360.00
  Acris Antibodies GmbH

CHAT

CHAT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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