CHAT antibody

Principal name

CHAT antibody

Alternative names for CHAT antibody

Choline O-acetyltransferase, Choline acetylase, CHOACTase, ChAT, EC=2.3.1.6

SwissProt ID

P13222 (Pig), P28329 (Human), P32738 (Rat), Q03059 (Mouse), Q90YJ9 (Chick)

Gene ID

1103 (CHAT), 396896, 12647 (Chat)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), GP (Guinea Pig), Rb (Rabbit), Mky (Monkey), Chk (Chicken), Prim (Primate), Can (Canine), Por (Porcine), Ze (Zebrafish), Chimp (Chimpanzee), Av (Birds)

Available hosts

Chicken, Mouse, Goat, Rabbit, Sheep

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoelectron Microscopy (EM)

Background of CHAT antibody

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.; Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.; CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.; Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.

General readings

Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. , Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. ,J Neurochem. 2006 Apr;97(2):515-26. Epub 2006 Mar 15. , PMID: 16539660 [PubMed - in process

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHAT (C-term) antibody

TA305830 (0.2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

CHAT (C-term) antibody

TA302990 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Chimp, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHAT (C-term) antibody

TA305830 (0.2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Western blot of rat brain lysate showing specific immunolabeling of the ~70k ChAT Goat IgG Aff - Purified Av, Chk, GP, Hu, Ms, non-Human Primates, Rt C, EM, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Western blot of rat brain lysate showing specific immunolabeling of the ~68/70k ChAT Rabbit IgG Serum Av, Chk, GP, Hu, Ms, non-Human Primates, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Western blot analysis of extracts of various cell lines, using CHAT antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHAT antibody

Immunofluorescence using ChAT antibody Cat.-No AP31801PU-N: A tissue Section throught an adult Mouse brain showing ChAT (Red staining) in cholinergic neurons of the caudate-putamen nucleus. The pale green staining is autofluorescence of Green Fluorescent Protein (GFP) in this transgenic animal. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P
0.1 mg / €440.00
  Acris Antibodies GmbH

CHAT (N-term) antibody

Confocal immunofluorescent analysis of CHAT Antibody (N-term) (Cat. #AP50885PU-N) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu, Ms ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CHAT antibody

CHAT Mouse IgG1 1E6 Ascites Hu, Rt C
0.1 ml / €490.00
  Acris Antibodies GmbH

CHAT antibody

CHAT Goat Serum GP, Hu, Ms, Rt C, WB
50 µl / €520.00
  Acris Antibodies GmbH

CHAT antibody

CHAT Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

CHAT antibody

CHAT Mouse IgG1 1H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CHAT antibody

CHAT polyclonal antibody ( Cat # PAB6736 ) ( 0.03 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CHAT antibody

The Chat polyclonal antibody ( Cat # PAB7033 ) ( 0.2 µg/ml ) staining of mouse brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CHAT antibody

Immunofluorescent staining of rat basal forebrain cholinergic neurons using CHAT polyclonal antibody ( Cat # PAB14313 ). Rabbit GP, Ms, Rb, Rt C, E, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

CHAT antibody

CHAT Rabbit GP, Ms, Rb, Rt E, P, WB
0.1 ml / €380.00
  2B Scientific Ltd.

CHAT antibody

Western blot analysis of CHAT expression in IMR32 (A); SKNSH (B) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt, Ze WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CHAT antibody

Western blot analysis of CHAT expression in IMR32 (A); SKNSH (B) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt, Ze WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CHAT antibody

Western blot analysis of CHAT expression in IMR32 (A); SKNSH (B) whole cell lysates. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt, Ze WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CHAT antibody

A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. Chicken Aff - Purified Hu, Ms C, ICC/IF, WB

Regular Price: 0.1 ml / €180.00

Special Price: 0.1 ml / €112.00

  Neuromics Antibodies

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