CHFR / RNF196 antibody

Principal name

CHFR / RNF196 antibody

Alternative names for CHFR / RNF196 antibody

E3 ubiquitin-protein ligase CHFR, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196

SwissProt ID

Q810L3 (Mouse), Q96EP1 (Human)

Gene ID

55743 (CHFR)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CHFR / RNF196 antibody

A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.

General readings

Scolnick DM & Halazonetis TD Chfr defines a mitotic stress checkpoint that delays entry into metaphase. Nature 406:430-5 (2000). PubMed PMID: 10935642

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHFR / RNF196 antibody

Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml] Mouse IgG1 1H3-A12 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CHFR / RNF196 antibody

TA302851 (2.5µg/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHFR / RNF196 antibody

Western blot analysis of extracts from Jurkat cells, using CHFR antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHFR / RNF196 antibody

WB Suggested Anti-CHFR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateCHFR is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CHFR / RNF196 antibody

CHFR / RNF196 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

CHFR / RNF196 antibody

Western Blot analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M08), clone 2B11.

Lane 1: CHFR transfected lysate(72.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2B11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CHFR / RNF196 antibody

CHFR polyclonal antibody ( Cat # PAB6325 ) staining ( 2.5 µg/ml ) of paraffin embedded human brain ( cerebellum ) .  Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu E, P
0.1 mg / €400.00
  Abnova Taiwan Corp.

CHFR / RNF196 antibody

Human Breast; Breast; CHFR antibody - N-terminal region (ARP43250_P050) in Human Breast cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CHFR / RNF196 antibody

NB100-1166 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, IP, P
0.1 mg / €400.00
  Novus Biologicals Inc.

CHFR / RNF196 antibody

Western Blot: CHFR Antibody [NBP1-30986] - Western blot analysis of CHFR on H1299, diluted at 1:1000 with 7.5% SDS PAGE gel. Rabbit IgG Hu Dot, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

CHFR / RNF196 antibody

Western Blot: CHFR Antibody [NBP1-52955] - This Anti-CHFR antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CHFR / RNF196 (transcript variant 1)

CHFR / RNF196 Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CHFR / RNF196

CHFR / RNF196 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CHFR / RNF196

CHFR / RNF196 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

CHFR / RNF196

CHFR / RNF196 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

CHFR / RNF196

CHFR / RNF196 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CHFR overexpression lysate

CHFR overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

CHFR overexpression lysate

CHFR overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CHFR overexpression lysate

CHFR overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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