CHK1 antibody

Principal name

CHK1 antibody

Alternative names for CHK1 antibody

CHEK1, CHEK-1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog

SwissProt ID

O14757 (Human), O35280 (Mouse), O61661 (Drome), P34208 (Schpo), P38147 (Yeast), Q6DE87 (Xenla), Q8AYC9 (Chick), Q91ZN7 (Rat), Q9N3Z3 (Caeel)

Gene ID

1111 (CHEK1), 852577 (CHK1), 12649 (Chek1), 140583

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Ye (Yeast)

Available hosts

Goat, Mouse, Rabbit, Sheep

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of CHK1 antibody

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

General readings

1. Gray JE  et al. A phase I, pharmacokinetic, and pharmacodynamic study of panobinostat, an HDAC inhibitor, combined with erlotinib in patients with advanced aerodigestive tract tumors.Clin Cancer Res 20:1644-55 (2014). IHC ; Human .
2. Mashukova A  et al. The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. J Cell Sci127:3568-77 (2014).
3. Fan S  et al. DIM (3,3'-diindolylmethane) confers protection against ionizing radiation by a unique mechanism. Proc Natl Acad Sci U S A 110:18650-5 (2013). WB ; Rat .
4. Zhang J  et al. NANOG modulates stemness in human colorectal cancer. Oncogene : (2012).PubMed: 23085761
5. Gao M  et al. A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity. PLoS One 7:e49687 (2012). WB ; Human .
6. Ravi D  et al. A network of conserved damage survival pathways revealed by a genomic RNAi screen. PLoS Genet 5:e1000527 (2009). WB ; Mouse .
7. Zou Y  et al. Characterization of nuclear localization signal in the N terminus of CUL4B and its essential role in cyclin E degradation and cell cycle progression. J Biol Chem 284:33320-32 (2009). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHK1 antibody

AP32963PU-N (2µg/ml) staining of Rat Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK1 antibody

  Rabbit polyclonal Chk1 (Ser296) antibody(Phospho-specific)  
Western blot analysis of extracts from HUVEC cells, treated with UV (15mins), using Chk1 (Phospho-Ser296) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit polyclonal Chk1 (Ser280) antibody(Phospho-specific)  
Western blot analysis of extracts from HeLa cells, using Chk1 (phospho-Ser280) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit polyclonal Chk1 (Ab-280) antibody  
Western blot analysis of extracts from JurKat cells, using Chk1 (Ab-280) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Anti-CHEK1 (Phospho-Ser280) Rabbit Polyclonal Antibody  
Predicted band size: 54 kDa . Positive control: HepG2 cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HepG2 cells untreated with UV lysate Lane2: HepG2 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 Antibody  
Western blot analysis of Chk1 expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 Antibody  
Western blot analysis of Chk1 expression in Raw264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 Antibody  
Western blot analysis of Chk1 expression in MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 Antibody  
Western blot analysis of Chk1 expression in UV treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 Antibody  
Western blot analysis on HeLa cell lysate using Chk1 Antibody Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 (Ser280) Antibody (Phospho-specific)  
Western blot analysis of Chk1 phosphorylation expression in UV treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 (Ser286) Antibody (Phospho-specific)  
Western blot analysis of Chk1 phosphorylation expression in Na3VO4 treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 (Ser296) Antibody (Phospho-specific)  
Western blot analysis of Chk1 phosphorylation expression in UV treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 (Ser301) Antibody (Phospho-specific)  
Western blot analysis of Chk1 phosphorylation expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 (Ser317) Antibody (Phospho-specific)  
Western blot analysis of Chk1 phosphorylation expression in MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1(Ser280) Antibody (Phospho-specific)  
Western blot analysis on HeLa cell lysate using Phospho-Chk1(Ser280) Antibody Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 (Ser280) Antibody (Phospho-specific)  
Western blot analysis on HeLa cell lysate using Phospho-Chk1(Ser280) Antibody Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Phospho-Chk1 (Ser345) Antibody (Phospho-specific)  
CHK1 Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Rabbit Polyclonal Chk1 Antibody  
Western blot analysis of Chk1 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK1 antibody

  Mouse Monoclonal Chk1 Antibody (2G1D5)  
Western Blot: Chk1 Antibody (2G1D5) [TA336271] - WB analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates. Mouse IgG1 2G1D5 Purified Hu C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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