CHK2 antibody

Principal name

CHK2 antibody

Alternative names for CHK2 antibody

CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog

SwissProt ID

O96017 (Human), Q9U1Y5 (Caeel), Q9UGF0 (Human), Q9Z265 (Mouse)

Gene ID

11200 (CHEK2), 1120031 (truncated-kdpA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mam (Mammalia), Can (Canine), Chimp (Chimpanzee), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Luminex (LMNX)

Background of CHK2 antibody

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

General readings

General / background references:Ahn J et al. Questioning the role of checkpoint kinase 2 in the p53 DNA damage response. J Biol Chem 278:20480-9 (2003). PubMed PMID: 12654916
Jallepalli PV et al. The Chk2 tumor suppressor is not required for p53 responses in human cancer cells. J Biol Chem 278:20475-9 (2003). PubMed PMID: 12654917
Stevens C et al. Chk2 activates E2F-1 in response to DNA damage. Nat Cell Biol 5:401-9 (2003). PubMed PMID: 12717439
Wu X & Chen J Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis. J Biol Chem 278:36163-8 (2003). PubMed PMID: 12855706
Tan S et al. Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity. Proc Natl Acad Sci U S A 100:11997-2002 (2003). PubMed PMID: 14514889
Lipton L et al. Contribution of the CHEK2 1100delC variant to risk of multiple colorectal adenoma and carcinoma. Cancer Lett 200:149-52 (2003). PubMed PMID: 14568168
Brody LC CHEKs and balances: accounting for breast cancer. Nat Genet 31:3-4 (2002). PubMed PMID: 11984555

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500384) Mouse IgG2b OTI2H5 Purified Can, Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385) Mouse IgG2b OTI4C10 Purified Can, Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500390) Mouse IgG1 OTI10G7 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500391) Mouse IgG1 OTI5C7 Purified Can, Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398) Mouse IgG1 OTI5C4 Purified Can, Hu F, ICC/IF, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500399) Mouse IgG1 OTI8D11 Purified Can, Hu, Mky F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500405). Mouse IgG2b OTI2F4 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2a 4B7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CHK2 antibody

Immunohistochemistry-Paraffin: Chk2 Antibody (5C4) [NBP1-47695] - Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-CHEK2mouse monoclonal antibody. (Dilution 1:50) Mouse 5C4 Aff - Purified Can, Hu F, ICC/IF, IP, P, WB
0.1 ml / €590.00
  Novus Biologicals Inc.

CHK2 (N-term) antibody

Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with Chk2 antibody at 1 ug /ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of extracts from Jurkat and HeLa cells treated with etoposide 25uM 24hours, using Chk2 (Ab-68) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 pThr68 antibody

Western blot using Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kDa corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin.  Chk2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center. Rabbit Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Predicted band size: 61kDa. Positive control: 293T , Hela and HT-29 cell lysate . Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of Chk2 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of extracts of various cell lines, using CHEK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

293 T cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with mouse anti-Chk2 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 CHK2909 Aff - Purified Hu WB
50 µl / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western Blot: Chk2 Antibody (73C175.1.1) [TA336399] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml. Mouse IgG1 73C175.1.1 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

CHEK2 ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278) Mouse IgG1 OTI2F4 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

CHEK2 ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278) Mouse IgG1 OTI4C10 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

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