CHK2 antibody

Principal name

CHK2 antibody

Alternative names for CHK2 antibody

CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog

SwissProt ID

O96017 (Human), Q9U1Y5 (Caeel), Q9UGF0 (Human), Q9Z265 (Mouse)

Gene ID

11200 (CHEK2), 50883 (Chek2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Mky (Monkey), Por (Porcine), Can (Canine), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Luminex (LMNX), Frozen Sections (C)

Background of CHK2 antibody

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

General readings

Clone DCS-273 is used in these references:
1. Syljuäsen, R.G., et al., Cancer Res. 66, 10253-10257 (2006)
2. Berger, M., et al., Mol. Cell Biol. 25, 5380-5388 (2005)
3. Latella, L., et al., Mol. Cell Biol. 24, 6350-6361 (2004)
4. Louria-Hayon, I., et al., J. Biol. Chem. 278, 33134-33141 (2003)
5. Lukas, C., et al., Cancer Res. 61, 4990-4993 (2001)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500384) Mouse IgG2b OTI2H5 Purified Can, Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385) Mouse IgG2b OTI4C10 Purified Can, Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500390) Mouse IgG1 OTI10G7 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500391) Mouse IgG1 OTI5C7 Purified Can, Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398) Mouse IgG1 OTI5C4 Purified Can, Hu F, ICC/IF, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500399) Mouse IgG1 OTI8D11 Purified Can, Hu, Mky F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500405). Mouse IgG2b OTI2F4 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2a 4B7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CHK2 (N-term) antibody

Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with Chk2 antibody at 1 ug /ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of extracts from HeLa cells using CHK2 (phospho-Ser516) antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of extracts from Jurkat cells, using Chk2 (Phospho-Thr387) Antibody (#TA312767).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Chk2 (Phospho-Thr383) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of the extracts from Jurkat cells untreated or treated with UV using Chk2 (Ab-68) Antibody and Chk2 (Phospho-Thr68) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of extracts from Jurkat and HeLa cells treated with etoposide 25uM 24hours, using Chk2 (Ab-68) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 pThr68 antibody

Western blot using Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kDa corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin.  Chk2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center. Rabbit Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Predicted band size: 61kDa. Positive control: 293T , Hela and HT-29 cell lysate . Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of Chk2 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of Chk2 phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CHK2 antibody

Western blot analysis of extracts of various cell lines, using CHEK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

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