CHK2 antibody

Principal name

CHK2 antibody

Alternative names for CHK2 antibody

CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog

SwissProt ID

O96017 (Human), Q9U1Y5 (Caeel), Q9UGF0 (Human), Q9Z265 (Mouse)

Gene ID

11200 (CHEK2), 50883 (Chek2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Mky (Monkey), Por (Porcine), Can (Canine), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Luminex (LMNX), Frozen Sections (C)

Background of CHK2 antibody

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

General readings

Clone DCS-273 is used in these references:
1. Syljuäsen, R.G., et al., Cancer Res. 66, 10253-10257 (2006)
2. Berger, M., et al., Mol. Cell Biol. 25, 5380-5388 (2005)
3. Latella, L., et al., Mol. Cell Biol. 24, 6350-6361 (2004)
4. Louria-Hayon, I., et al., J. Biol. Chem. 278, 33134-33141 (2003)
5. Lukas, C., et al., Cancer Res. 61, 4990-4993 (2001)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHK2 antibody

  CHEK2 (CHK2) mouse monoclonal antibody, clone OTI10G7 (formerly 10G7)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500390) Mouse IgG1 OTI10G7 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

  CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398) Mouse IgG1 OTI5C4 Purified Can, Hu F, ICC/IF, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

  CHEK2 (CHK2) mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)  
Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500405). Mouse IgG2b OTI2F4 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CHK2 antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 antibody Cat.-No AM06242SU-N Mouse IgG2b 1C12B8 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €460.00
  OriGene Technologies GmbH

CHK2 pThr383 antibody

Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody AP55715PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €340.00
  OriGene Technologies GmbH

CHK2 pThr383 antibody

Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody AP55715PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €270.00
  OriGene Technologies GmbH

CHK2 pThr383 antibody

Immunofluorescence analysis of HeLa cells, using Chk2 (Phospho-Thr383) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

CHK2 pThr383 antibody

Immunofluorescence analysis of HeLa cells, using Chk2 (Phospho-Thr383) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

8 Item(s)

per page
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