CHK2 antibody

Principal name

CHK2 antibody

Alternative names for CHK2 antibody

CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog

SwissProt ID

O96017 (Human), Q9U1Y5 (Caeel), Q9UGF0 (Human), Q9Z265 (Mouse)

Gene ID

11200 (CHEK2), 50883 (Chek2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Mky (Monkey), Por (Porcine), Can (Canine), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Luminex (LMNX), Frozen Sections (C)

Background of CHK2 antibody

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

General readings

Clone DCS-273 is used in these references:
1. Syljuäsen, R.G., et al., Cancer Res. 66, 10253-10257 (2006)
2. Berger, M., et al., Mol. Cell Biol. 25, 5380-5388 (2005)
3. Latella, L., et al., Mol. Cell Biol. 24, 6350-6361 (2004)
4. Louria-Hayon, I., et al., J. Biol. Chem. 278, 33134-33141 (2003)
5. Lukas, C., et al., Cancer Res. 61, 4990-4993 (2001)

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  2. Highly Validated (7)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CHK2 antibody

  Mouse Monoclonal Chk2 Antibody (73C175.1.1)  
Western Blot: Chk2 Antibody (73C175.1.1) [TA336399] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml. Mouse IgG1 73C175.1.1 Purified Hu, Ms C, P, WB
0.1 mg / €334.00
  OriGene Technologies, Inc.

CHK2 antibody

  ELISA and Luminex validated CHEK2 mouse monoclonal antibody (clone 2F4, clone OTI2F4 (formerly 2F4)  
CHEK2 ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278) Mouse IgG1 OTI2F4 Purified Hu E, LMNX
0.1 mg / €350.00
  OriGene Technologies, Inc.

CHK2 antibody

  ELISA and Luminex validated biotinylated CHEK2 mouse monoclonal antibody (clone 4C10), clone OTI4C10 (formerly 4C10)  
CHEK2 ELISA with 2F4 Capture (TA600006) and 4C10 Detection (TA700006) Antibodies. Substrate used: Recombinant Human CHEK2 (TP301278) Mouse IgG1 OTI4C10 Purified Hu E, LMNX
50 µg / €350.00
  OriGene Technologies, Inc.

CHK2 antibody

Figure 1. AM00536AF-N CHK2 antibody Immunohistochemical staining of Human Intestine Paraffin Embedded Section Mouse IgG2a DCS-270 Azide Free Hu IP, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CHK2 antibody

AM06003PU-N CHEK2 antibody staining of Formalin-Fixed,  Paraffin-Embedded Human Spleen at 10 µg/ml followed by Biotin anti-Mouse IgG secondary antibody, Alkaline Phosphatase-streptavidin and Chromogen. Mouse IgG1 73C175.1.1 Purified Hu P, WB
50 µg / €380.00
  Acris Antibodies GmbH

CHK2 antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 antibody Cat.-No AM06242SU-N Mouse IgG2b 1C12B8 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €460.00
  Acris Antibodies GmbH

CHK2 antibody

Figure 1. AM20032AF-N CHK2 antibody Immunohistochemical staining of Human Intestine Paraffin Embedded Section. Mouse IgG2a DCS-273 Azide Free Hu IP, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CHK2 pSer516 antibody

Western blot (WB) analysis of p-Chk2 (pSer516) antibody Cat.-No.: AP01555PU-N in extracts from HeLa cells treated with UV Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 pThr68 antibody

Figure 2. Immunohistochemistry (IHC) analyzes of p-Chk2 antibody (pThr68) in paraffin-embedded human breast carcinoma tissue.  (Cat.-No.: AP01556PU-N) Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 pSer516 antibody

Figure 1. Western blot analysis of extracts from HeLa cells using Chk2 (phospho-Ser516) antibody (AP02432PU). Rabbit Aff - Purified Hu WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CHK2 pSer516 antibody

Figure 1. Western blot analysis of extracts from HeLa cells using Chk2 (phospho-Ser516) antibody (AP02432PU). Rabbit Aff - Purified Hu WB
50 µg / €240.00
  Acris Antibodies GmbH

CHK2 pThr68 antibody

CHK2 Rabbit IgG Purified Hu, Mky, Ms, Rt E, WB
50 µg / €370.00
  Acris Antibodies GmbH

CHK2 antibody

Western blot (WB) analysis of Chk2 antibody (Cat.-No.: AP06051PU-N) at 1/500 dilution Lane1:Hela cell lysate Lane2:Mouse liver tissue lysate Lane3:Rat spleen tissue lysate Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 antibody

Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using CHK2 antibody (Cat.-No. AP06052PU-N). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 antibody

Immunohistochemistry (IHC) analyzes of Chk2 antibody (Cat.-No.: AP06380PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 pSer379 antibody

Western blot (WB) analysis of Chk2 antibody (Cat.-No.: AP06512PU-N) in extracts from COS7 cells treated with UV 30'. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €380.00
  Acris Antibodies GmbH

CHK2 pThr387 antibody

Immunohistochemistry (IHC) analyzes of p-CHK2 (pThr387) antibody (Cat.-No.: AP20931PU-N) in paraffin-embedded human lung adenocarcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 pThr68 antibody

CHK2 Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

CHK2 antibody

CHK2 Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

Items 21 to 40 of 77 total

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