Cholera Haemolysin antibody

Principal name

Cholera Haemolysin antibody

Alternative names for Cholera Haemolysin antibody

hlyA, VC_A0219

SwissProt ID

P09545 (Vibch)

Gene ID

2612877 (VCA0219)

Available reactivities

Bac (Bacteria)

Available hosts


Available applications

Enzyme Immunoassay (E)

Background of Cholera Haemolysin antibody

The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.

2 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cholera Haemolysin antibody

Cholera Haemolysin Mouse IgG1 2024/251 Purified E
0.2 mg / €400.00
  Acris Antibodies GmbH

Cholera Haemolysin antibody

Cholera Haemolysin Mouse IgG1 2024/251 Purified Bac E
0.1 mg / €360.00
  Novus Biologicals Inc.

2 Item(s)

per page
  • LinkedIn