Hu (Human), Ms (Mouse), Chk (Chicken), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Choline kinase alpha antibody
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Glunde,K., (2008) Cancer Res. 68 (1), 172-180
CHKA polyclonal antibody (A01), Lot # GPC1060208QCS1 Western Blot analysis of CHKA expression in A-431 ( Cat # L015V1 ).
Hum. Adult Placenta; Host: Rabbit. Target Name: CHAD . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; CHKA antibody - middle region (ARP53583_P050) in Hum. Adult Placenta cells using Western Blot
Western Blot: Choline kinase alpha Antibody [NBP1-85630] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.