CKAP5 antibody

Principal name

CKAP5 antibody

Alternative names for CKAP5 antibody

Ch-TOG protein, KIAA0097, Cytoskeleton-associated protein 5, Colonic and hepatic tumor over-expressed protein

SwissProt ID

A2AGT5 (Mouse), Q14008 (Human), Q9CUN0 (Mouse)

Gene ID

9793 (CKAP5)

Available reactivities

Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat), Rb (Rabbit), Bov (Bovine), Can (Canine)

Available hosts

Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of CKAP5 antibody

CKAP5 is a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CKAP5 antibody

Specificity of polyclonal antibodies against ch-TOGp. Total cell protein (;50 mg) from human HeLa cells was immunoblotted with a rabbit polyclonal serum generated against the C-terminal 301 amino acids of the ch-TOGp. Rabbit Purified Hu, Mam, Ms, Rt ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CKAP5 (C-term) antibody

HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against ch-TOG. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

CKAP5 (C-term) antibody

HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against ch-TOG. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu WB
50 µl / €190.00
  Acris Antibodies GmbH

CKAP5 antibody

Western blot analysis of CKAP5 in human HeLa cells lysate ( 50 mg ) using CKAP5 polyclonal antibody ( Cat # PAB12456 ). Rabbit Hu, Ms, Rt ICC/IF, IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

CKAP5 antibody

HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against ch-TOG. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu WB
0.2 ml / €420.00
  Novus Biologicals Inc.

CKAP5 antibody

CKAP5 Rabbit Serum Hu, Mam, Ms, Rt ICC/IF, IP, WB
25 µl / €170.00
  Novus Biologicals Inc.

CKAP5 antibody

Western Blot: ch-TOG Antibody [NBP1-06513] - Western Blot and Immunoprecipitation: ch-TOG antibody [NBP1-06513]- Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ch-TOG antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ch-TOG was also immunoprecipitated by rabbit anti-ch-TOG antibodies NBP1-06511and NBP1-06512, which recognize upstream epitopes. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

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