CKBB antibody

Principal name

CKBB antibody

Alternative names for CKBB antibody

Creatine kinase B-type, Creatine kinase B chain, B-CK, Creatine kinase BB, CKB

SwissProt ID

P00567 (Rabit), P05122 (Chick), P05124 (Canfa), P07335 (Rat), P12277 (Human), Q04447 (Mouse), Q29594 (Pig), Q5EA61 (Bovin)

Gene ID

1152 (CKB), 12709 (Ckb), 24264

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of CKBB antibody

Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes.
Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.

General readings

Chang EJ., et al. (2008) Nat Med. 14(9):966-72.
Debrincat MA., et al. (2007) J Biol Chem. 282(7):4728-37.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CKBB antibody

  Rabbit Polyclonal antibody to Creatine kinase (brain) (creatine kinase, brain)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308722 diluted at 1:10000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CKBB antibody

  Goat Polyclonal Antibody against CKB / Brain Creatine Kinase  
TA302453 (0.1µg/ml) staining of human brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CKBB antibody

  Mouse monoclonal Creatine Kinase BB Antibody (Center)  
Creatine Kinase BB (CKB) Antibody (Center) (Cat. #TA325138) western blot analysis in MDA-MB453,293,Y79 cell line ,mouse stomach and brain tissue lysates (35ug/lane).This demonstrates the CKB antibody detected the CKB protein (arrow). Mouse IgG1 893CT29.1.1 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CKBB antibody

  Mouse monoclonal anti-Creatine Kinase BB (CKB) antibody (Center)(Ascites)  
Creatine Kinase BB (CKB) Antibody (Center) (Cat. #TA328113) western blot analysis in MDA-MB-453,293 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the Creatine Kinase BB (CKB) antibody detected the Creatine Kinase BB (CKB) protein (arrow). Mouse IgG1 893CT29.1.1 Ascites Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CKBB (N-term) antibody

CKBB Mouse IgG2b CK-BYK/21E10 Biotin Hu, Ms, Rb, Rt C, ICC/IF, P, WB
50 µg / €290.00
  Acris Antibodies GmbH

CKBB (N-term) antibody

CKBB Mouse IgG2b CK-BYK/21E10 Purified Hu, Ms, Rb, Rt C, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CKBB (N-term) antibody

The anti-CKB Pab is used in Western blot to detect CKB in mouse colon tissue lysate (Lane 1) and Y79 cell lysate (Lane 2). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CKBB antibody

The anti-CKB Pab is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CKBB antibody

CKBB Mouse IgG1 BGN/2ba6 Purified Hu E, R
0.2 mg / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CKB - human gene knockout kit via CRISPR

CKB - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CKB - human gene knockout kit via CRISPR

CKB - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CKB - human gene knockout kit via CRISPR

CKB - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CKB - human gene knockout kit via CRISPR

CKB - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ckb - mouse gene knockout kit via CRISPR

Ckb - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ckb - mouse gene knockout kit via CRISPR

Ckb - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ckb - mouse gene knockout kit via CRISPR

Ckb - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ckb - mouse gene knockout kit via CRISPR

Ckb - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CKBB

  Recombinant protein of human creatine kinase, brain (CKB)  
CKBB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CKBB (1-381, His-tag)

CKBB Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

CKBB (1-381, His-tag)

CKBB Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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