CKMT antibody

Principal name

CKMT antibody

Alternative names for CKMT antibody

CKMT1A, CKMT1B, U-MtCK, Mia-CK, Creatine kinase U, ubiquitous mitochondrial Creatine kinase, Acidic-type mitochondrial creatine kinase

SwissProt ID

P12532 (Human), P25809 (Rat), P30275 (Mouse), P70079 (Chick), Q29577 (Pig), Q9TTK8 (Bovin)

Gene ID

1159 (CKMT1B)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of CKMT antibody

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Haas. R.C., et al. (1989) J Biol Chem 264(5): 2890-2897 Stachowiak. O., et al. (1998) Mol Cell Biochem 184(1-2): 141-151Lipskaya. T.Y. (2001) Biochemistry Mosc 66(10): 1098-1111

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CKMT antibody

Western blot analysis of CKMT1B over-expressed 293 cell line, cotransfected with CKMT1B Validated Chimera RNAi ( Cat # H00001159-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CKMT1B monoclonal antibody (M04), clone 2C8 (Cat # H00001159-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG2a 2C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CKMT antibody

Sample(30 ug whole cell lysate). A:H1299. B:HeLa S3. 10% SDS PAGE. TA308867 diluted at 1:1000 Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CKMT antibody

Predicted cell location: Cytoplasm. Positive control: Human lung and breast cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CKMT1A/CKMT1B antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CKMT antibody

Predicted band size: 50 kDa . Positive control: Mouse large intestine tissue lysate. Recommended dilution: 1/1000-3000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CKMT antibody

Host: Rabbit; Target Name: CKMT1B; Sample Tissue: RPMI-8226 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CKMT antibody

CKMT Mouse IgG1 1A6-C7-G10 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CKMT antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKMT1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT17A2 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CKMT antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKMT1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT17A2 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CKMT (N-term) antibody

Western blot analysis using AP13637PU-N CKMT1 antibody to detect CKMT1 in Mouse colon tissue lysate (Lane 1) and ZR-75-1 cell lysate (Lane 2). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CKMT antibody

CKMT1B polyclonal antibody (A01), Lot # 060613JCS1 Western Blot analysis of CKMT1B expression in 293 ( Cat # L026V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CKMT antibody

Western Blot analysis of CKMT1B expression in transfected 293T cell line (H00001159-T01) by CKMT1B MaxPab polyclonal antibody.

Lane 1: CKMT1B transfected lysate(45.87 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CKMT antibody

CKMT Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

CKMT antibody

CKMT1B monoclonal antibody (M16), clone 2C9. Western Blot analysis of CKMT1B expression in A-431(Cat # L015V1 ). Mouse IgG1 2C9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CKMT antibody

The CKMT1A polyclonal antibody ( Cat # PAB4614 ) is used in Western blot to detect CKMT1A in mouse colon tissue lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CKMT antibody

Western Blot: Creatine kinase MT 1B Antibody [NBP1-31543] - Western blot analysis of NBP1-31543 on H1299 (lane A) and HeLa S3 (lane B), with 10% SDS PAGE, diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CKMT

CKMT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CKMT (40-417, His-tag)

CKMT Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

CKMT (40-417, His-tag)

CKMT Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Antibody Pairs

Catalog No.    

CKMT1B (Human) IP-WB Antibody Pair

CKMT1B (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CKMT1A overexpression lysate

CKMT1A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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