CLIC1 antibody

Principal name

CLIC1 antibody

Alternative names for CLIC1 antibody

NCC27, Chloride channel ABP, hRNCC, Nuclear chloride ion channel 27, Regulatory nuclear chloride ion channel protein, Chloride intracellular channel protein 1

SwissProt ID

O00299 (Human), Q29238 (Pig), Q5E9B7 (Bovin), Q6MG61 (Rat), Q95MF9 (Rabit), Q9Z1Q5 (Mouse)

Gene ID

1192 (CLIC1), 114584 (Clic1), 406864

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Can (Canine), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Gt (Goat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of CLIC1 antibody

Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6). The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associate to the plasma membrane) via their single transmembrane domain. When membrane associated, CLIC channels indeed function as ion channels (ebling the passage of Cl ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend whether CLICs are membrane bound or cytosolic. CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration.

General readings

Warton K, et al. (2002) J Biol Chem. 277(29):26003-11.
Singh H,et al. (2007) FEBS J. 274(24):6306-16.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CLIC1 antibody

  Rabbit Polyclonal Anti-CLIC1 Antibody  
Lanes: ; Lane 1: 30ug mouse renal epithelial lysate; Lane 2: 30ug mouse renal epithelial lysate; Lane 3: 30ug mouse renal epithelial lysate; Primary Antibody Dilution: ; 1:500; Secondary Antibody: ; Anti-rabbit-HRP; Secondary Antibody Dilution: ; 1:2500; Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3F9 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CLIC1 (45-57) antibody

  Goat Anti-CLIC1 / NCC27 (aa45-57) Antibody  
TA326744 (2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

  Goat Anti-CLIC1 / NCC27 Antibody  
TA326745 (2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

  Rabbit polyclonal anti-CLIC1 antibody (Center)  
CLIC1 Antibody (Center) (Cat. #TA328109) western blot analysis in HL-60,MCF-7,Raji,WiDr cell line and human placenta tissue lysates (35ug/lane).This demonstrates the CLIC1 antibody detected the CLIC1 protein (arrow). Rabbit Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

  Rabbit Polyclonal Anti-CLIC1  
Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates: 1- 4. Anti-CLIC1 antibody , (1:200). 5- 8. Anti-CLIC1 antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CLIC1 antibody

  Rabbit Polyclonal Anti-CLIC1 Antibody  
WB Suggested Anti-CLIC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

  Rabbit Polyclonal Anti-CLIC1 Antibody  
Lanes: ; Lane 1: 30ug mouse renal epithelial lysate; Lane 2: 30ug mouse renal epithelial lysate; Lane 3: 30ug mouse renal epithelial lysate; Primary Antibody Dilution: ; 1:500; Secondary Antibody: ; Anti-rabbit-HRP; Secondary Antibody Dilution: ; 1:2500; Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

  Rabbit Polyclonal Anti-CLIC1 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human testis tissue, Primary antibody: (CLIC1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

  Rabbit Polyclonal CLIC1 Antibody  
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu P
50 µg / €325.00
  OriGene Technologies, Inc.

CLIC1 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2D4 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CLIC1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CLIC1 antibody

CLIC1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CLIC1 - human gene knockout kit via CRISPR

CLIC1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CLIC1 - human gene knockout kit via CRISPR

CLIC1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CLIC1 - human gene knockout kit via CRISPR

CLIC1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CLIC1 - human gene knockout kit via CRISPR

CLIC1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Clic1 - mouse gene knockout kit via CRISPR

Clic1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Clic1 - mouse gene knockout kit via CRISPR

Clic1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Clic1 - mouse gene knockout kit via CRISPR

Clic1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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