CNOT2 antibody

Principal name

CNOT2 antibody

Alternative names for CNOT2 antibody

CDC36, NOT2, HSPC131, MSTP046, CCR4-NOT transcription complex subunit 2, CCR4-associated factor 2

SwissProt ID

Q8C5L3 (Mouse), Q9NZN8 (Human)

Gene ID

4848 (CNOT2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of CNOT2 antibody

CNOT2 (CCR4-NOT transcription complex subunit 2) is a ubiquitous protein encoded by the human gene CNOT2. CNOT2 belongs to the CNOT2/3/5 family and is part of the CCR4-NOT complex. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits (e.g. CNOT1, CNOT2) are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. Increased expression of the CNOT2 subunit acts to strongly repress transcription by RNA polymerase II. This repressive effect is mediated by a conserved NOT-Box, which is located at the C-terminus of CNOT2 proteins. Repression by the NOT-Box is sensitive to treatment with the histone deacetylase (HDAC) inhibitor trichostatin A.

General readings

Albert T.K., Lemaire M., van Berkum N.L., Gentz R., Collart M.A., Timmers H.T.M. Nucleic Acids Res. 28:809-817(2000)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CNOT2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808626)(1:150) Mouse IgG2b OTI5A12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT2 antibody

Western blot analysis of extracts from HepG2 cells, treated with starved (24hours), using CNOT2 (Ab-101) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CNOT2 antibody

WB Suggested Anti-CNOT2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Liver Rabbit IgG Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNOT2 antibody

WB Suggested Anti-CNOT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CNOT2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808695)(1:150) Mouse IgG2a OTI6D7 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CNOT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808696)(1:150) Mouse IgG2b OTI9B10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT2 pSer101 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using CNOT2 (Phospho-Ser101) antibody AP55827PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €340.00
  Acris Antibodies GmbH

CNOT2 pSer101 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using CNOT2 (Phospho-Ser101) antibody AP55827PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €270.00
  Acris Antibodies GmbH

CNOT2 antibody

CNOT2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CNOT2 antibody

Western Blot analysis of CNOT2 expression in transfected 293T cell line (H00004848-T01) by CNOT2 MaxPab polyclonal antibody.

Lane 1: CNOT2 transfected lysate(59.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CNOT2 antibody

Detection limit for recombinant GST tagged CNOT2 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 2E10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

CNOT2 antibody

CNOT2 monoclonal antibody (M08), clone 3F1 Western Blot analysis of CNOT2 expression in A-549 ( Cat # L025V1 ). Mouse IgG2a 3F1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CNOT2 antibody

CNOT2 monoclonal antibody (M08), clone 3F1 Western Blot analysis of CNOT2 expression in A-549 ( Cat # L025V1 ). Mouse IgG2a 3F1 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

CNOT2 pSer101 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using CNOT2 (Phospho-Ser101) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

CNOT2 pSer101 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using CNOT2 (Phospho-Ser101) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

CNOT2 antibody

Western blot analysis of extracts from HepG2 cells, treated with starved 24h, using CNOT2 (Ab-101) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

CNOT2 antibody

Western blot analysis of extracts from HepG2 cells, treated with starved 24h, using CNOT2 (Ab-101) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

CNOT2 (Isoform 2) antibody

Human Liver; WB Suggested Anti-CNOT2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: Human Liver; CNOT2 antibody - middle region (ARP32048_T100) in Human Liver cells using Western Blot Rabbit IgG Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

CNOT2 antibody

Human THP-1; WB Suggested Anti-CNOT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; CNOT2 antibody - middle region (ARP32721_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CNOT2 antibody

Western Blot: CNOT2 Antibody [NBP1-30454- Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CNOT2 antibody NBP1-30454 used for IP at 10 mcg/mg lysate. Rabbit Hu IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

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