CNOT4 antibody

Principal name

CNOT4 antibody

Alternative names for CNOT4 antibody

NOT4Hp, NOT4, CCR4-NOT transcription complex subunit 4, E3 ubiquitin-protein ligase CNOT4, CCR4-associated factor 4, Potential transcriptional repressor NOT4Hp

SwissProt ID

O95628 (Human), Q8BT14 (Mouse)

Gene ID

4850 (CNOT4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Xen (Xenopus)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of CNOT4 antibody

The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits within the complex are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. CNOT4 (CCR4-NOT transcription complex subunit 4), also known as CCR4-associated factor 4 and E3 ubiquitin-protein ligase CNOT4, is a 575 amino acid protein that is a subunit of the CCR4-NOT complex. CNOT4 contains one C3H1-type zinc finger, one RING-type zinc finger and one RRM (RNA recognition motif) domain. Via its RING domain, CNOT4 binds E2 ubiquitin ligases. CNOT4 functions as a UbcH5B-dependent ubiquitin-protein ligase (E3 ligase). There are eight isoforms of CNOT4 that are expressed as a result of alternative splicing events.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CNOT4 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CNOT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800157) Mouse IgG1 OTI1H2 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 (N-term) antibody

Western blot analysis of CNOT4 (arrow) using rabbit polyclonal CNOT4 Antibody (N-term) (RB11916). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CNOT4 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNOT4 antibody

Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNOT4 antibody

Western Blot: CNOT4 Antibody [TA336994] - analysis of CNOT4 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using CNOT4 antibody at 0.05 ug/ml. Rabbit Purified Bov, Chk, Hu, Ms, Rt, Xen P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CNOT4 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CNOT4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG1 OTI3D11 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI3A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI2A5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI4B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI4C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI2H4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI3B8 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CNOT4 Mouse monoclonal antibody. (TA800048; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI1F10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI2F10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI1F9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI1G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Mouse IgG1 OTI7D7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CNOT4 (C-term) antibody

Western blot analysis of CNOT4 Antibody (C-term) (AP11452PU-N) in CEM cell line lysates (35 µg/lane). CNOT4 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CNOT4 antibody

Immunohistochemistry (IHC) analyzes of CNOT4 antibody (Cat.-No.: AP21144PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CNOT4 (N-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CNOT4 Control Peptide

CNOT4
50 µg / €160.00
  Acris Antibodies GmbH

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